本文選題：鈦種植體　切入點：生物學老化　出處：《浙江大學》2015年博士論文　論文類型：學位論文

【摘要】：純鈦種植體與周圍骨組織之間能否建立快速穩(wěn)固的結合主要取決于種植體表面的理化性狀和生物學活性。近年來的研究發(fā)現(xiàn)鈦種植體表面在制備完成之后的保存過程中會經(jīng)歷一個生物學老化的過程,使得種植體表面細胞黏附、增殖和分化能力顯著降低,從而導致種植體骨整合能力的下降。本研究聯(lián)合應用液體浸泡保存和紫外線照射的方法處理多孔純鈦種植體表面,并對處理后的各組表面進行表征以及體內(nèi)外生物學效應評價。 首先,制備出新鮮多孔鈦表面(SLAnew),在空氣中保存4周的多孔鈦表面(SLAold),在水中保存4周的多孔鈦表面(modSLA),在空氣或水中保存4周后經(jīng)紫外線處理的多孔鈦表面(UV-SLA, UV-modSLA),共5組表面并分別進行表征。結果顯示在水中保存可使鈦表面保持親水性并有效隔絕碳氫化合物的污染,且在鈦表面自動生成了一層直徑約15nm的致密納米級顆粒；紫外線照射可賦予鈦片表面超親水性,高效清除鈦片表面的碳氫化合物,并使鈦片表面獲得羥基自由基和Ti4+陽離子活性位點。 在上述5組鈦片表面培養(yǎng)MC3T3-E1細胞,觀察比較細胞在各組表面的黏附、增殖和分化情況。細胞實驗結果顯示,鈦片在空氣中保存4周后,表面的細胞黏附、增殖和分化能力均顯著降低；在水中保存則生物學活性下降不明顯；紫外線處理能恢復并進一步提升老化鈦表面的生物學活性；浸泡保存形成的納米形貌也有助于鈦片表面細胞的早期增殖與分化,在水中保存4周后具備了納米結構再經(jīng)過紫外線處理的UV-modSLA表面,表現(xiàn)出最強的生物學活性。 然后,我們將上述5組表面的種植體每組32枚,共計160枚,植入40只新西蘭兔的股骨和脛骨干骺端,分別于植入后3周和6周處死,取股骨干骺端的標本進行扭力測試,取脛骨干骺端的標本制作硬組織切片觀察。動物實驗結果顯示,經(jīng)過紫外線照射處理的兩組鈦種植體表面的種植體-骨接觸率(BIC)和骨量(BA)顯著高于其他組；UV-modSLA種植體表現(xiàn)出最高的扭力峰值。 本研究探索了多孔純鈦種植體表面制備完成之后在不同媒介中保存4周并經(jīng)過紫外線照射處理前后的理化性狀和體內(nèi)外生物學活性變化情況,發(fā)現(xiàn)多孔純鈦表面在儲存過程中會受到不同程度的碳氫化合物污染,水中保存的鈦表面受到碳污染程度相對較輕且出現(xiàn)了納米級結構；紫外線處理能清除鈦表面的碳氫化合物污染,恢復并進一步增強鈦表面的生物學活性。在水中保存4周后具備了納米結構再經(jīng)過紫外線處理的UV-modSLA種植體,表現(xiàn)出最佳的生物學活性和骨性結合強度,為臨床開發(fā)新一代種植體提供了新思路和新方向。
[Abstract]:Between the titanium implant and the surrounding bone tissue can physicochemical properties and biological activity of a rapid stable combination mainly depends on the implant surface. Recent studies have found that titanium implant surface preservation process after the completion of the preparation process will experience a biological aging, makes the implant surface cell adhesion. The proliferation and differentiation ability decreased significantly, which leads to a decrease in the implant bone integration ability. The research method of combination of liquid preservation and ultraviolet irradiation treatment of porous titanium implant surface, and to evaluate the characterization and in vivo biological effects on the treated surface of each group.
First of all, prepared fresh porous titanium (SLAnew), preservation of porous titanium surface for 4 weeks in the air (SLAold), preservation of porous titanium 4 weeks in water (modSLA), stored in the air or water for 4 weeks after the treatment of porous titanium surface ultraviolet (UV-SLA, UV-modSLA), a total of 5 groups the surface was characterized respectively. The results showed that the titanium surface to keep the preservation of hydrophilic and effectively isolate hydrocarbon pollution in the water, and the automatic generation of dense titanium nano particles with a layer of about 15nm in diameter; UV radiation can give the titanium surface super hydrophilic, remove hydrocarbons titanium surface efficiently and so, the titanium surface to obtain hydroxyl radical and cationic Ti4+ active sites.
On the surface of Titanium 5 groups of cultured MC3T3-E1 cells were observed in the surface of each cell adhesion, proliferation and differentiation of cells. The experimental results show that after 4 weeks of titanium film preservation in the air, the surface of the cell adhesion, proliferation and differentiation ability were significantly decreased; preservation of biological activity in the water decreased significantly; ultraviolet treatment can restore and improve the aging biological activity of titanium surface; early nanotopography preservation formation also contributes to the surface of titanium cell proliferation and differentiation, preserved after 4 weeks with nano structure by UV-modSLA surface ultraviolet treatment in water showed the strongest biological activity.
Then, we will be in the 5 groups on the surface of the implant with 32 medals, a total of 160 medals, with 40 New Zealand Rabbits Femoral and tibial metaphysis, respectively, 3 and 6 weeks after implantation to death, the femoral metaphysis were torsion test, the tibial metaphyseal specimen making hard group weaving sections. Animal experiments showed that the implant bone after ultraviolet irradiation treatment of two groups of titanium implant surface contact rate (BIC) and bone mineral density (BA) was significantly higher than that of other groups; UV-modSLA implants showed the highest peak torque.
This study explored after porous titanium implant surface preparation in different media and stored for 4 weeks after the physicochemical changes of characteristics and in vivo biological activity before and after UV irradiation, found that the porous titanium surface will be affected by different degree of carbon hydrogen compounds pollution in the process of storage, preservation of the water surface by titanium carbon pollution level is relatively low and the emergence of the nano structure; UV treatment can remove the titanium surface hydrocarbon pollution, recovery and further enhance the biological activity of titanium surface. After 4 weeks with the preservation of nano structure after UV-modSLA implant ultraviolet treatment in water showed the best biological activity and bone bonding strength that provides a new direction for the development of a new generation of clinical implant.

【學位授予單位】：浙江大學
【學位級別】：博士
【學位授予年份】：2015
【分類號】：R783.1

【參考文獻】

相關期刊論文 前1條

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本文編號：1646662