本文選題：小鼠　切入點(diǎn)：-硝基喹啉--氧化物　出處：《暨南大學(xué)學(xué)報(bào)(自然科學(xué)與醫(yī)學(xué)版)》2017年06期 　論文類型：期刊論文

【摘要】：目的:建立小鼠舌癌模型,篩選誘癌率高及模擬人類發(fā)病的舌癌模型.方法:以Babl/c小鼠為實(shí)驗(yàn)對(duì)象,通過(guò)單獨(dú)和聯(lián)合應(yīng)用4-硝基喹啉-1-氧化物(4NQO)和檳榔堿(Arecoline)來(lái)誘發(fā)舌癌,以涂布法采用質(zhì)量濃度5mg/mL 4NQO;自然飲水法采用質(zhì)量濃度20、50、100μg/mL 4NQO,采用質(zhì)量濃度300μg/mL Arecoline及聯(lián)合質(zhì)量濃度100μg/mL 4NQO和質(zhì)量濃度300μg/mL Arecoline.各組誘癌劑飲用至16或14周時(shí),改為飲用滅菌自來(lái)水觀察至第40周,于誘癌的不同節(jié)點(diǎn)選取狀態(tài)不佳,體質(zhì)量下降超過(guò)20%的小鼠處死,取其舌頭、食管、胃、肝、脾臟組織標(biāo)本,通過(guò)肉眼和組織學(xué)觀察腫瘤發(fā)生發(fā)展情況.結(jié)果:自然飲水法質(zhì)量濃度50μg/mL 4NQO組70.0%(7/10)為輕度不典型增生,40.0%(4/10)為中度不典型增生,20.0%(2/10)為重度不典型增生,10.0%(1/10)為原位癌;聯(lián)合應(yīng)用質(zhì)量濃度為100μg/mL 4NQO和質(zhì)量濃度為300μg/mL Arecoline組10.0%(1/10)為輕度不典型增生,20.0%(2/10)為中度不典型增生,40.0%(4/10)為重度不典型增生,20.0%(2/10)為原位癌,50.0%(5/10)為浸潤(rùn)癌,30.0%(3/10)淋巴結(jié)轉(zhuǎn)移,20.0%(2/10)食管不典型增生.結(jié)論:質(zhì)量濃度50μg/mL的4NQO飲用16周繼續(xù)觀察至40周是舌癌癌前病變動(dòng)物模型建立的理想時(shí)間;質(zhì)量濃度為100μg/mL的4NQO飲用14周后改飲用自來(lái)水至40周癌變發(fā)展緩慢、典型,發(fā)病率高,組織病理學(xué)特征與人相似.聯(lián)合運(yùn)用低劑量Arecoline和4NQO是誘癌率最高的建模方法,且可觀察到頸部淋巴結(jié)轉(zhuǎn)移及食管病變,但癌變進(jìn)展快,不宜作為模擬人舌癌發(fā)病的模型.
[Abstract]:Objective: to establish a model of tongue cancer in mice, and to screen a model of tongue cancer with high carcinogenesis rate and mimic human disease. Methods: Babl/c mice were used to induce tongue cancer by using 4NQO (4-nitroquinoline-1-oxide) and Arecoline alone and in combination with arecoline. 5 mg / mL 4NQO was used for coating method, 20 渭 g / mL 4NQO for natural drinking water, 300 渭 g / mL Arecoline and combined concentration of 100 渭 g / mL 4NQO and 300 渭 g / mL Arecoline for 16 or 14 weeks. After drinking sterilized tap water for 40 weeks, the mice whose body mass decreased by more than 20% were killed at the different nodes of cancer induction, and their tongue, esophagus, stomach, liver and spleen were taken out, and the tissues of tongue, esophagus, stomach, liver and spleen were collected. Results: the mass concentration of 50 渭 g / mL 4NQO group (70.010 / 10) was mild atypical hyperplasia (40.010%) and moderate atypical hyperplasia (20.0% / 10) (severe dysplasia 10.0 / 110). Combined use of 100 渭 g / mL 4NQO and 300 渭 g / mL Arecoline group (10.0 / 10) as mild atypical hyperplasia 20.0 / 10) as moderate atypical hyperplasia (40.010 / 10) as severe atypical hyperplasia 20.0% / 10) as carcinoma in situ (50.0% / 510) as invasive carcinoma 30.00.10 / 10) lymph node metastasis 20.0r.r.210. Conclusion: it is an ideal time to establish the animal model of precancerous lesions of the tongue after drinking 4NQO at a concentration of 50 渭 g / mL for 16 weeks to 40 weeks. The carcinogenesis of 4NQO with 100 渭 g / mL 4NQO for 14 weeks to 40 weeks after drinking was slow, typical, high incidence, histopathological characteristics were similar to that of human. The combination of low dose Arecoline and 4NQO was the best method to induce cancer. Cervical lymph node metastasis and esophageal lesions can be observed, but the carcinogenesis is rapid, so it is not suitable to be used as a model for simulating human tongue cancer.
【作者單位】： 廣西醫(yī)科大學(xué)護(hù)理學(xué)院基礎(chǔ)教研室;廣西醫(yī)科大學(xué)病理生理學(xué)教研室;廣西醫(yī)科大學(xué)病理教研室;
【基金】：廣西自然科學(xué)基金資助項(xiàng)目(2013GXNSFAA019244)
【分類號(hào)】：R-332;R739.86

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 黎明武;李晶;于大海;卿海云;閉似嫦;;4-NQO飲水法不同給藥時(shí)間誘發(fā)小鼠舌癌模型的比較[J];中國(guó)臨床新醫(yī)學(xué);2012年07期

，

本文編號(hào)：1636832