發(fā)布時(shí)間：2018-03-19 12:27

  本文選題：納米羥基磷灰石　切入點(diǎn)：阿倫膦酸鈉I型膠原蛋白　出處：《福建醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：考察納米羥基磷灰石（nano-hydroxyapatite，，nHA）涂層表面阿倫膦酸鈉（alendronate sodium，AL）和I型膠原蛋白（collagen type I，COL I）對(duì)成骨細(xì)胞生長(zhǎng)、黏附和增殖的影響。方法：采用電化學(xué)沉積技術(shù)在純鈦（pure titanium，PT）表面沉積nHA涂層，通過掃描電子顯微鏡（scanning electron microscope，SEM）、X射線能譜分析儀（energy dispersive X-ray spectroscopy，EDS）、X射線衍射儀（X-ray diffraction，XRD）和傅里葉紅外光譜儀（fourier transform infraredspectroscopy，F(xiàn)TIR）表征。而后采用化學(xué)結(jié)合法在nHA涂層上制備nHA/AL、nHA/COL I、nHA/AL/COL I和nHA/COL I/AL復(fù)合膜，通過接觸角測(cè)量?jī)x（contactangle meter，CAM）、X射線光電子能譜儀（X-ray photoelectron spectrometer，XPS）和FTIR進(jìn)行表征。在實(shí)驗(yàn)組：nHA/AL/COL I和nHA/COL I/AL復(fù)合膜及對(duì)照組：nHA涂層、nHA/AL和nHA/COL I復(fù)合膜表面培養(yǎng)成骨細(xì)胞，通過SEM觀察成骨細(xì)胞形態(tài)，WST-1檢測(cè)復(fù)合膜對(duì)成骨細(xì)胞生長(zhǎng)和增殖的影響。結(jié)果：nHA涂層表面細(xì)胞增殖能力最低，與各復(fù)合膜之間的差異均有統(tǒng)計(jì)學(xué)意義（P0.05），nHA/COL I復(fù)合膜的增殖能力稍高于nHA/AL復(fù)合膜（P0.05），nHA/AL/COL I和nHA/COL I/AL復(fù)合膜的增殖活性均高于對(duì)照組（P0.05），但nHA/AL/COL I與nHA/COL I/AL復(fù)合膜的細(xì)胞增殖活性的差異無統(tǒng)計(jì)學(xué)意義（P0.05）。nHA/AL/COL I和nHA/COL I/AL復(fù)合膜表面成骨細(xì)胞充分伸展，多層細(xì)胞形成致密的板層樣結(jié)構(gòu)，nHA/AL和nHA/COL I復(fù)合膜表面細(xì)胞重疊生長(zhǎng)，而nHA涂層表面細(xì)胞較少，可見大面積nHA基底。結(jié)論：10-6M AL和COL I均能促進(jìn)成骨細(xì)胞的黏附和增殖，兩者共同作用時(shí)作用更強(qiáng)。nHA表面AL和COL I的結(jié)合次序?qū)Τ晒羌?xì)胞的生長(zhǎng)和增殖能力無明顯影響。種植體表面AL和COL I的聯(lián)合應(yīng)用值得進(jìn)一步研究探索。
[Abstract]:Objective: to investigate the effects of alendronate sodium alendronate (ALL) and collagen I collagen I (Col I) on the growth, adhesion and proliferation of osteoblasts on the surface of nano-hydroxyapatite nano-hydroxyapatite (NHA) coating. Scanning electron microscopy (SEM) and scanning electron microscopy (SEM) were used to characterize the electron microscopesimetry (SEM) and X-ray diffraction (dispersive) dispersive X-ray spectrometer (EDR) and Fourier transform infrared spectroscopy (FTIR). Then the composite films of nHAR / ALNHACOL / InHAP / COL and nHA/COL / IRA were prepared on nHA coating by chemical combination method. X-ray photoelectron spectrometerus XPSs and FTIR were used to characterize the osteoblasts on the surface of the experimental group and the control group. The osteoblasts were cultured on the surface of the NHA / all / Col / nHA/COL / IAL composite membrane and the control group (n / nHA coating / nHAAL / nHA/COL I composite membrane). The effect of the composite membrane on the growth and proliferation of osteoblasts was observed by SEM. The proliferative activity of P0.05 / Col I composite membrane was slightly higher than that of nHA/AL composite membrane P0.05 / all / Col I and nHA/COL I / AL composite membrane, but the cell proliferation activity of nHA/AL/COL I / nHA/COL I / AL composite membrane was higher than that of the control group. There was no statistically significant difference in the number of osteoblasts on the surface of P0.05nHA / all / Col I and nHA/COL / I / AL composite membranes. The multilayer cells formed a dense lamellar structure, the cells on the surface of NHA / AL and nHA/COL I composite membrane superposed, but there were few cells on the surface of nHA coating, which showed that there was a large area of nHA substrate. Conclusion\% 10 ~ (-6) M AL and COL I can promote the adhesion and proliferation of osteoblasts. The binding order of AL and COL I on the surface of. NHA has no significant effect on the growth and proliferation of osteoblasts. The combined application of AL and COL I on implant surface is worthy of further study.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783.6


本文編號(hào)：1634268