本文選題：燒結(jié)　切入點(diǎn)：摻鍶透鈣磷石　出處：《華北理工大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的通過材料的性能檢測、生物活性判斷、影像學(xué)檢查來評價(jià)摻鍶透鈣磷石骨水泥修復(fù)家兔牙槽骨缺損的能力,觀察堿性成纖維細(xì)胞生長因子(Basic Fibroblast Growth Factor,b-FGF)和骨保護(hù)素(Osteoprotegerin,OPG)在骨缺損區(qū)的表達(dá)和分布情況,探討摻鍶透鈣磷石骨水泥的作用機(jī)制,研究摻鍶透鈣磷石骨水泥作為骨替代材料的可行性,為臨床應(yīng)用提供理論依據(jù)。方法制備透鈣磷石,并按Sr/(Sr+Ca)為5%和10%加入氯化鍶,制備摻鍶透鈣磷石骨水泥。通過X線衍射儀(X-ray diffraction,XRD)、掃描電鏡(Scanning electron microscope,SEM)、萬能材料實(shí)驗(yàn)機(jī)等對材料的物相、表面形態(tài)和抗壓強(qiáng)度進(jìn)行測量和分析。采用急性溶血性實(shí)驗(yàn)和皮膚刺激反應(yīng)試驗(yàn)檢測材料的安全性及生物相容性。健康家兔36只隨機(jī)分成四組,分別為空白對照組、透鈣磷石組、摻鍶5%透鈣磷石組和摻鍶10%透鈣磷石組,每組動(dòng)物9只。所有動(dòng)物均建立雙側(cè)下頜牙槽骨門齒遠(yuǎn)端無牙區(qū)10×3×4mm骨缺損,空白組缺損處不充填任何材料,其余實(shí)驗(yàn)各組分別充填相對應(yīng)的骨水泥塊。術(shù)后分別于4,8,12周處死每組動(dòng)物各3只,通過CBCT觀察影像學(xué)變化,分析觀察缺損區(qū)b-FGF、OPG兩種因子的表達(dá)與分布情況,利用Image-Pro Plus6.0軟件測量同一倍數(shù)下b-FGF、OPG陽性表達(dá)部位的平均光密度值(MOD值),各組間進(jìn)行比較,統(tǒng)計(jì)學(xué)處理。結(jié)果1成功制備摻鍶透鈣磷石骨水泥,衍射結(jié)果分析鍶以磷酸氫鍶的形式存在于骨水泥中;電鏡圖片顯示樣品由大量短棒狀、片狀、團(tuán)簇狀的晶體結(jié)構(gòu)組成,結(jié)構(gòu)間有大小不等的微孔;鍶的摻入降低了透鈣磷石的抗壓強(qiáng)度,且與摻入鍶的濃度相關(guān),即摻鍶10%透鈣磷石的強(qiáng)度低于摻鍶5%透鈣磷石強(qiáng)度。2生物相容性實(shí)驗(yàn)結(jié)果示三組骨水泥溶血率均低于國家要求標(biāo)準(zhǔn),體外試驗(yàn)和體內(nèi)試驗(yàn)均證明符合材料安全,無毒。3 4周時(shí)摻鍶5%和10%組骨水泥開始溶解、吸收;透鈣磷石組骨水泥未溶解;空白組缺損明顯,無新生骨組織。8周時(shí)摻鍶透鈣磷石的兩組缺損已基本修復(fù),修復(fù)早于未摻鍶組,且摻鍶10%透鈣磷石組較摻鍶5%透鈣磷石組修復(fù)效果好,新生骨面光滑平整;透鈣磷石組缺損區(qū)稍凹于周圍骨組織,新生骨表面不光滑;空白組缺損區(qū)為凹坑狀,無新生骨組織。12周時(shí)空白組未完成缺損的修復(fù);透鈣磷石組缺損區(qū)基本修復(fù),形成骨小梁等結(jié)構(gòu);摻鍶組修復(fù)完成。4免疫組化結(jié)果分析:組內(nèi)比較時(shí),b-FGF和OPG的陽性表達(dá)都隨著時(shí)間的延長呈下降趨勢。b-FGF的表達(dá),4周時(shí),各組間比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05),其中空白組MOD值最小,摻鍶10%透鈣磷石組MOD值最大;8周時(shí),各組間比較差異均有統(tǒng)計(jì)學(xué)意義(P0.05),摻鍶5%透鈣磷石組MOD值最大,摻鍶組高于透鈣磷石組,空白組MOD值最小;12周時(shí)四組差異無統(tǒng)計(jì)學(xué)意義(P0.05)。OPG在4周和8周時(shí)的MOD值均為摻鍶5%透鈣磷石組最高,摻鍶10%透鈣磷石組高于透鈣磷石組,空白對照組最小,各組之間差異有統(tǒng)計(jì)學(xué)意義(P0.05);12周時(shí),空白組和透鈣磷石組比較差異無統(tǒng)計(jì)學(xué)意義(P0.05),摻鍶5%透鈣磷石組和摻鍶10%組比較差異無統(tǒng)計(jì)學(xué)意義(P0.05),后兩組和前兩組交叉比較時(shí)差異有統(tǒng)計(jì)學(xué)意義(P0.05),后兩組MOD值較高。析因分析結(jié)果顯示,MOD值的變化既與骨水泥的作用時(shí)間直接相關(guān),又與組別差異相關(guān),同時(shí)二者之間具有交互作用。鍶的摻入可以使b-FGF和OPG的表達(dá)增加,促進(jìn)成骨。結(jié)論1本實(shí)驗(yàn)所采用骨水泥的制備方法可靠,能簡單復(fù)制,且材料的抗壓強(qiáng)度與松質(zhì)骨強(qiáng)度相近。2透鈣磷石、摻鍶5%、10%透鈣磷石骨水泥基本無毒性,為較安全的植入材料。3摻鍶透鈣磷石骨水泥能夠在修復(fù)早期促進(jìn)b-FGF和OPG的增加,促進(jìn)成骨過程。4摻鍶5%和摻鍶10%透鈣磷石骨水泥能縮短缺損的修復(fù)時(shí)間,且摻鍶量為10%時(shí),較摻鍶5%透鈣磷石修復(fù)效果明顯。
[Abstract]:Objective through the performance testing of materials, biological activity judgment, imaging examination to evaluate the ability of strontium doped calcium phosphate through bone cement in repairing rabbit alveolar bone defects, basic fibroblast growth factor (Basic Fibroblast, Growth Factor, b-FGF) and osteoprotegerin (Osteoprotegerin, OPG) expression and distribution in the area of bone defect the mechanism of strontium brushite bone cement, feasibility study of strontium brushite bone cement as bone substitute materials, provide a theoretical basis for clinical application. Methods preparation of brushite, according to Sr / (Sr+Ca) were 5% and 10% with strontium chloride, preparation of doped strontium brushite bone cement. Through X-ray diffraction (X-ray diffraction, XRD), scanning electron microscopy (Scanning electron, microscope, SEM), universal material test machine of material phase, the measurement and analysis of surface morphology and compressive strength. The acute hemolysis Experiment and skin irritation and safety of biological reaction test material compatibility. 36 healthy rabbits were randomly divided into four groups, namely control group, brushite group, strontium 5% brushite and strontium 10% brushite group, each group of animal 9. All animal models were established double lateral incisor distal alveolar bone in edentulous area of 10 * 3 * 4mm bone defect, the defect of the blank group were not filled with any material, the rest of the experimental groups were bone water mud filling corresponding. After operation respectively in 4,8,12 weeks killed 3 rats in each group animal, histological changes were observed by CBCT image analysis, observe the defect area b-FGF, the expression and distribution of OPG two factor, measure the same multiples of b-FGF using Image-Pro Plus6.0 software, the average optical density of OPG positive expression value (MOD value), comparison between groups were statistically. Results 1 successful preparation of strontium brushite bone cement, diffraction. The result analysis in strontium strontium hydrogen phosphate in the form of bone cement; electron microscopy images showed that the sample is composed of a large number of short rod, sheet, composed of crystal structure of cluster, structure between the micropore sizes; strontium incorporation decreases the compressive strength of brushite, and with the incorporation of strontium concentration that is, strontium 10% brushite strength is lower than 5% strontium brushite strength.2 biocompatibility test results showed that three groups of bone cement hemolysis rate was lower than the national standard requirements, the in vitro and in vivo experiments are proved to meet the material safety, non-toxic strontium 5%.3 at 4 weeks and 10% groups of bone cement begin to dissolve the absorption; brushite bone cement group dissolved; control group was two group without defect defect, new bone tissue at.8 weeks of strontium brushite has been restored, not earlier than the repair of strontium and strontium 10% group, brushite group than strontium 5% through calcium P Zuxiu stone complex effect, new 鐢熼闈㈠厜婊戝鉤鏁，

本文編號：1614396