本文選題：口腔正畸　切入點：白介素-17　出處：《重慶醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 用SD大鼠建立正畸牙根吸收模型，探討白介素-17在不同作用力及不同加力時間條件下牙根吸收區(qū)的表達(dá)變化。 方法 選擇SD雄性成年大鼠，要求月齡相同、體重相近，建立正畸牙根吸收模型，按不同正畸作用力分為偽實驗組、40g、60g、80g三個組，每組根據(jù)加力時間分為1d、4d、7d、14d四個亞組。采用連續(xù)切片行蘇木精-伊紅染色、抗酒石酸酸性磷酸酶染色觀察牙根吸收情況。應(yīng)用免疫組織化學(xué)方法及雙抗夾心酶聯(lián)免疫吸附法(ELISA）觀察白介素-17的表達(dá)變化情況。 結(jié)果 1）牙根吸收區(qū)域主要表達(dá)在根中1/3，相同力值不同加力時間的情況下，牙根吸收在加力第4d時已經(jīng)發(fā)生，到第7d逐漸達(dá)到峰值，第14d牙根吸收程度逐漸下降，牙本質(zhì)修復(fù)現(xiàn)象在部分根吸收陷窩出現(xiàn)；加力時間相同不同力值的情況下，隨著正畸力值的增加，，牙根吸收的程度不斷加重； 2）偽實驗組未見白介素-17陽性表達(dá)。相同力值不同加力時間條件下，表現(xiàn)為第1d主要表達(dá)為弱陽性，隨時間增加，第7d時達(dá)到峰值，表現(xiàn)為強(qiáng)陽性，隨后至第14d，表達(dá)強(qiáng)度逐漸下降，表現(xiàn)為中度陽性。相同時間力值越大，陽性表達(dá)強(qiáng)度越明顯。白介素-17的表達(dá)與加力時間和力值之間的相關(guān)性具有統(tǒng)計學(xué)意義（P0.05),且與力值大小呈正相關(guān)； 3）牙根吸收面積在不同加力時間和力值大小條件下，與白介素-17陽性表達(dá)率呈正相關(guān)（P0.05)，有統(tǒng)計學(xué)意義。 結(jié)論 1）牙根吸收程度在不同加力時間及力值大小時存在規(guī)律性，力值越大，牙根吸收越嚴(yán)重；在一個加力周期內(nèi)，牙根吸收程度從第4d到第7d呈現(xiàn)上升趨勢，7d時達(dá)到峰值，隨后逐漸下降，第14d牙根吸收區(qū)出現(xiàn)牙本質(zhì)修復(fù)現(xiàn)象； 2）白介素-17量的變化與牙根吸收變化趨勢相同。正畸牙周組織改建過程中有白介素-17參與，提示臨床操作中應(yīng)注意加力大小和周期的控制； 3）白介素-17可能參與正畸牙根吸收過程。
[Abstract]:Purpose. A orthodontic root resorption model was established in SD rats to investigate the expression of interleukin-17 (IL-17) in the root resorption region under different applied forces and time. Method. Adult SD male rats were selected to establish the orthodontic root resorption model with the same age and similar body weight. According to different orthodontic forces, the rats were divided into three groups: the pseudo-experimental group (40g / 60g) and the control group (80g). Each group was divided into four subgroups according to the time of exertion. The subgroups were stained with hematoxylin and eosin by serial sections. The root resorption was observed by tartrate-resistant acid phosphatase staining and the expression of interleukin-17 was observed by immunohistochemical method and double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results. 1) the root resorption region was mainly expressed in 1 / 3 of the root, and the root resorption occurred at the 4th day after the same force and reached the peak at the 7th day, and gradually decreased at the 14th day. Dentin repair appeared in some root resorption lacunae, and the degree of root resorption increased with the increase of orthodontic force. 2) No positive expression of interleukin-17 was found in the pseudo-experimental group. The expression of interleukin-17 was mainly weak positive on the 1st day, and increased with time, and reached the peak at the 7th day, and showed strong positive at the 7th day. Then to the 14th day, the expression intensity gradually decreased, and the expression was moderately positive. The greater the force value was at the same time, The stronger the positive expression was, the more significant the correlation between the expression of interleukin-17 and the time of exertion and the value of force was significant (P 0.05), and positive correlation was found between the expression of interleukin-17 and the value of force. 3) there was a positive correlation between the root resorption area and the positive rate of interleukin-17 expression under the conditions of different applied time and force value, which had statistical significance. Conclusion. 1) the degree of root resorption was regular at different applied time and force value. The greater the force value was, the more serious the root resorption was, and the root resorption degree increased from the 4th day to the 7th day, and reached the peak value at 7 days. At the 14th day, dentin repair appeared in the root resorption area. 2) the change of interleukin-17 is the same as the change of root absorption. Interleukin-17 is involved in the process of orthodontic periodontal tissue remodeling, which suggests that attention should be paid to the control of stress and cycle in clinical operation. 3) Interleukin-17 may be involved in the root resorption process of orthodontic teeth.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R783.5

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