本文選題：靜電紡絲　切入點(diǎn)：自噬　出處：《吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2016年05期 　論文類型：期刊論文

【摘要】：目的:觀察載藥聚乳酸(PLA)靜電紡絲膜對(duì)口腔鱗狀細(xì)胞癌CAL-27細(xì)胞的作用,探討預(yù)防口腔癌術(shù)后復(fù)發(fā)的方法。方法:利用靜電紡絲技術(shù)制備載順鉑(DDP)PLA靜電紡絲膜(DDP/PLA膜)、載氯喹(CQ)/順鉑/氯喹PLA靜電紡絲膜(CQ/DDP/CQ/PLA膜,復(fù)合膜)和載氯喹/順鉑PLA靜電紡絲膜(CQ/DDP/PLA膜,共混膜)。通過(guò)掃描電鏡(SEM)觀察3種載藥PLA靜電紡絲膜的表面形態(tài);紫外分光光度分析法測(cè)定PLA靜電紡絲膜的降解速率;激光共聚焦顯微鏡觀察自噬特異性蛋白LC3-Ⅱ在CAL-27細(xì)胞中的表達(dá)情況;MTT法檢測(cè)CAL-27細(xì)胞生存率。結(jié)果:SEM觀察,DDP/PLA膜、復(fù)合膜和共混膜納米纖維連續(xù),表面光滑,直徑較均勻;載藥PLA靜電紡絲膜在21d左右降解;第1天DDP/PLA膜、復(fù)合膜和共混膜對(duì)CAL-27細(xì)胞殺傷作用不明顯,第3天以后,DDP/PLA膜組、復(fù)合膜組及共混膜組CAL-27細(xì)胞的生存率明顯降低(P0.05);復(fù)合膜組和共混膜組LC3-Ⅱ熒光亮度均低于DDP/PLA膜組。結(jié)論:具有緩釋效果的CQ/DDP/PLA納米靜電紡絲膜提高了CAL-27細(xì)胞對(duì)DDP的敏感性,增強(qiáng)了DDP對(duì)口腔癌CAL-27細(xì)胞的殺傷作用。
[Abstract]:Objective: to observe the effect of drug-loaded polylactic acid (PLA) electrospun membrane on oral squamous cell carcinoma (CAL-27) cells. Methods: the electrospinning technique was used to prepare DDP / PLA membrane carrying cisplatin and DDP / PLA / chloroquine / chloroquine PLA electrospun membrane. The surface morphology of three kinds of drug-loaded PLA electrospinning films were observed by scanning electron microscope (SEM), and the degradation rate of PLA electrospun films was determined by UV spectrophotometry. The expression of autophagy specific protein LC3- 鈪，

本文編號(hào)：1570269