發(fā)布時(shí)間：2018-03-02 15:16

  本文選題：血管再生　切入點(diǎn)：交感神經(jīng)　出處：《福建醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:骨修復(fù)改建活動(dòng)復(fù)雜而有序,與血管生成密不可分,它們相互影響和緊密聯(lián)系。以往認(rèn)為骨代謝的調(diào)節(jié)涉及骨局部的自分泌、旁分泌以及內(nèi)分泌等因素,然而越來越多的研究指出交感神經(jīng)系統(tǒng)在骨形成和骨吸收過程中也發(fā)揮關(guān)鍵的作用。本研究通過檢測失交感神經(jīng)支配下拔牙窩骨愈合過程中VEGF及CD31的表達(dá)特點(diǎn),進(jìn)一步探討血管再生在交感神經(jīng)調(diào)節(jié)骨愈合過程中的可能作用。材料和方法:利用改良頸上神經(jīng)切除術(shù)方法切除SD大鼠一側(cè)頸上神經(jīng)節(jié),建立失交感神經(jīng)支配的動(dòng)物模型。拔除SD大鼠失交感神經(jīng)側(cè)(SCGX側(cè))與交感神經(jīng)支配側(cè)(CG側(cè))上頜第一磨牙,建立拔牙窩骨愈合動(dòng)物模型。分別于拔牙術(shù)后1、3、7、14和21天5個(gè)時(shí)間點(diǎn)處死動(dòng)物。通過免疫組化技術(shù)檢測CD31的表達(dá),測定血管內(nèi)皮細(xì)胞數(shù)目。并檢測拔牙窩底1/3、中1/3、頂1/3在拔牙后不同時(shí)間點(diǎn)VEGF的表達(dá)水平,測定其平均光密度值,進(jìn)一步探討交感神經(jīng)信號(hào)調(diào)節(jié)新骨形成和骨改建過程VEGF時(shí)空表達(dá)特點(diǎn)對(duì)血管再生的可能作用。結(jié)果:1、SD大鼠頸上神經(jīng)節(jié)切除側(cè)表現(xiàn)出明顯的上瞼下垂的交感神經(jīng)功能抑制的癥狀,免疫組化染色證實(shí)切除組織為頸上神經(jīng)節(jié)組織。通過拔除SCGX側(cè)及CG側(cè)上頜第一磨牙,成功建立拔牙窩骨愈合模型。2、CD31免疫組化檢測結(jié)果表明:拔牙后第1天,SCGX組拔牙窩內(nèi)CD31陽性染色的血管內(nèi)皮細(xì)胞數(shù)量較CG組略多,但差異無統(tǒng)計(jì)學(xué)意義(P0.05);SCGX組在術(shù)后第3天、7天、14天時(shí)陽性表達(dá)的血管內(nèi)皮細(xì)胞數(shù)量較CG組增加顯著,兩組差異有統(tǒng)計(jì)學(xué)意義(P0.05),在第21天時(shí)兩組血管內(nèi)皮細(xì)胞數(shù)量均下降,且兩組數(shù)目接近,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3、VEGF表達(dá)的平均光密度值檢測結(jié)果表明:1)拔牙窩底1/3VEGF的表達(dá):術(shù)后第1天,SCGx組的VEGF表達(dá)較對(duì)照組略高,但差異無統(tǒng)計(jì)學(xué)意義(P0.05);隨后VEGF表達(dá)增強(qiáng),且第3天、7天、14天,兩組差異具有統(tǒng)計(jì)學(xué)意義(P0.05),而在第21天,兩組差異無統(tǒng)計(jì)學(xué)意義(P0.05)。2)拔牙窩中1/3VEGF的表達(dá),SCGX組與CG組在術(shù)后第1天,表達(dá)較弱,兩組之間差異無統(tǒng)計(jì)學(xué)意義(P0.05),在拔牙后第3天、7天、14天、21天SCGx組的VEGF表達(dá)高于CG組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。3)拔牙窩頂1/3VEGF的表達(dá),SCGX組與CG組在術(shù)后第1天,第3天,兩組之間差異無統(tǒng)計(jì)學(xué)意義(P0.05),拔牙后第7天、14天、21天SCGx組的VEGF表達(dá)高于CG組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。4)在拔牙窩VEGF整體表達(dá)來看,SCGX組與CG組在術(shù)后第1天,兩組之間差異無統(tǒng)計(jì)學(xué)意義(P0.05),在拔牙后第3天、7天、14天、21天兩組差異具有統(tǒng)計(jì)學(xué)意義(P0.05),整個(gè)拔牙窩的VEGF表達(dá)情況與拔牙窩中1/3相同。5)CG組的拔牙窩底部1/3與頂部1/3VEGF平均光密度比較,術(shù)后第1天、14天兩組間差異沒有統(tǒng)計(jì)學(xué)意義(P0.05),在術(shù)后第3天、7天、21天具有統(tǒng)計(jì)學(xué)意義(P0.05)。6)SCGX組的SD大鼠拔牙窩底部1/3與頂部1/3VEGF平均光密度比較,在術(shù)后第1天、3天、7天、21天,兩位點(diǎn)間差異有統(tǒng)計(jì)學(xué)意義(P0.05),而14天,兩位點(diǎn)差異無統(tǒng)計(jì)意義(P0.05)。結(jié)論:失交感神經(jīng)支配調(diào)節(jié)拔牙窩愈合新骨形成的過程,可能是通過促進(jìn)拔牙窩VEGF的表達(dá),促進(jìn)拔牙窩新骨形成區(qū)毛細(xì)血管新生,增加血管數(shù)量來改善拔牙窩的供血以改善微循環(huán),從而促進(jìn)新骨形成。
[Abstract]:Objective: bone repair and reconstruction of complex and orderly, and angiogenesis are closely related, they affect each other and close contact. In the past that the regulation of bone metabolism involves autocrine bone, paracrine and endocrine factors, but more and more research is also pointed out that play a key role in the absorption process of the sympathetic nervous system in bone formation and bone. Expression of VEGF and CD31 in this study through the detection of sympathetic denervation by extraction socket healing process, to further explore the possible role in regulating bone vascular regeneration of sympathetic nerve in the healing process. Materials and methods: the cervical nerve resection by resection of SD rats to establish animal model of superior cervical ganglion, loss of sympathetic the innervation of the sympathetic nerve loss. Removal of SD rat side (SCGX side) and the sympathetic innervation side (CG side) of maxillary first molar, the establishment of the animal model of bone healing extraction sockets . respectively after extraction of 1,3,7,14 and 21 days 5 time points were animal. Using immunohistochemistry to detect the expression of CD31, vascular endothelial cell number and detection. The sockets at the bottom of the 1/3, 1/3, top 1/3 in the extraction at different time points after the expression of VEGF, determine the average optical density value, further study of sympathetic signal regulation of new bone formation and remodeling of the spatial and temporal expression of VEGF characteristics of the possible role of vascular regeneration. Results: 1 SD rat superior cervical ganglion resection side showed obvious function of sympathetic nerve ptosis inhibitory symptoms, immunohistochemical staining for tissue resection of superior cervical ganglion by tissue. Remove the SCGX side and the CG side of the maxillary first molar, successfully established the extraction socket healing model.2, CD31 immunohistochemistry results showed that: first days after extraction, SCGX extraction fossa CD31 positive staining the number of endothelial cells was lower than that of CG 緇勭暐澶，

本文編號(hào)：1557054