本文關(guān)鍵詞： 納米網(wǎng)層鈦材料 紫外光處理 納米銀粒子 抗菌活性 大鼠骨髓間充質(zhì)干細胞　出處：《南方醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究背景和目的:鈦材料由于其能夠與人體骨組織產(chǎn)生骨結(jié)合,以及具有優(yōu)良的機械性能和抗腐蝕性能,被廣泛應(yīng)用于口腔的臨床治療。然而,無論是短期的種植體手術(shù)術(shù)后感染,還是長期的種植體周圍炎,均可能導(dǎo)致種植治療的失敗。因此,賦予鈦種植體抗菌性是一種預(yù)防種植體周感染、提高種植術(shù)成功率的方法。高濃度堿處理能夠在鈦材料表面形成疏松多孔的納米級網(wǎng)層狀結(jié)構(gòu),被稱為納米網(wǎng)層鈦材料。此材料雖然擁有優(yōu)良的促成骨分化能力,但缺乏對細菌入侵的抵抗能力。UV處理鈦材料不僅能夠帶來更快更完整的骨結(jié)合,還能夠減少細菌早期附著。另外,以不同方式加載納米銀粒子的鈦材料也顯示了長效的抗菌性和較好的細胞相容性。本研究用UV處理和加載納米銀粒子兩種方式,制備兩種具有不同時效性的抗菌性鈦材料,并評價其抗菌能力以及對大鼠骨髓間充質(zhì)干細胞的影響。方法:經(jīng)過研磨的純鈦片浸泡于10 M NaOH溶液中室溫處理24小時后,用高強度短時間的UV照射制備成UV處理納米網(wǎng)層鈦材料,以及硝酸銀溶液浸泡UV照射的方式在納米網(wǎng)層鈦材料表面加載納米銀離子。運用掃描電鏡(SEM)、X射線光電子能譜分析(XPS)、接觸角測量等方法,分析兩種納米網(wǎng)層鈦材料的表面特征。以齦下菌斑早期定植細菌Actinomycesoris MG-1作為菌種評價兩種納米網(wǎng)層鈦材料的抗菌能力。另外,在兩種抗菌性納米網(wǎng)層鈦材料表面培養(yǎng)大鼠骨髓間充質(zhì)干細胞以分析其成骨分化活性。研究結(jié)果:1、高強度短時間UV照射使納米網(wǎng)層鈦材料獲得超親水性,并有效去除表面碳污染;以硝酸銀溶液浸泡UV照射還原的方式,成功制備加載納米銀離子納米網(wǎng)層鈦材料。2、UV處理納米網(wǎng)層鈦材料有效地抵抗Actinomycesoris MG-1的早期附著,并且抑制其生物膜形成達6小時。3、載銀納米網(wǎng)層鈦材料不僅能夠殺滅已附著細菌,抑制生物膜形成,并且顯示出長效抗菌性達28天。4、UV處理納米網(wǎng)層鈦材料促進大鼠骨髓間充質(zhì)干細胞的早期附著與增殖、ALP活性、骨鈣素分泌以及細胞外基質(zhì)礦化,成骨相關(guān)基因(ALP、Runx2、BMP、OPN)表達水平均高于納米網(wǎng)層鈦材料。5、載銀納米網(wǎng)層鈦材料與納米網(wǎng)層鈦材料相比,抑制大鼠骨髓間充質(zhì)干細胞的早期附著與增殖以及ALP活性。結(jié)論:UV處理納米網(wǎng)層鈦材料能夠抗菌達6小時以上,并且能夠促進大鼠骨髓間充質(zhì)干細胞的成骨分化,載銀納米網(wǎng)層鈦材料保持抗菌性達28天,但有一定程度的細胞毒性。本研究將進一步深入探討增強載銀納米網(wǎng)層鈦材料的生物相容性。
[Abstract]:Background and objective: titanium is widely used in clinical treatment of oral cavity due to its ability to bind bone to human bone tissue, as well as its excellent mechanical properties and corrosion resistance. Both short-term post-implant infections and long-term peri-implant infections can lead to failure in implant therapy. Therefore, giving titanium implants antimicrobial properties is a way to prevent peri-implant infection. The method of improving the success rate of implantation. High concentration alkali treatment can form porous and porous nano-mesh layered structure on the surface of titanium, which is called nano-mesh layer titanium material, although this material has excellent ability to promote bone differentiation. But the lack of resistance to bacterial invasion. UV treatment of titanium not only leads to faster and more complete bone bonding, but also reduces the early attachment of bacteria. Titanium materials loaded with silver nanoparticles in different ways also showed long-term antibacterial properties and good cytocompatibility. In this study, two kinds of antimicrobial titanium materials with different time-efficiency were prepared by UV treatment and loading of nano-silver particles. To evaluate its antibacterial ability and its effect on rat bone marrow mesenchymal stem cells. Methods: after being soaked in 10 M NaOH solution at room temperature for 24 hours after grinding pure titanium slices. Titanium nanowires were prepared by UV irradiation with high intensity and short time. The silver nanoparticles were loaded on the surface of titanium nanowire by soaking in silver nitrate solution. The X-ray photoelectron spectroscopy (XPS) analysis and contact angle measurement were used to analyze the silver ions on the surface of titanium nanowet layer by means of scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurement, etc. The surface characteristics of two kinds of nanometer-netted titanium materials were analyzed. The antibacterial ability of the two nano-netted titanium materials was evaluated by early colonization bacteria (Actinomycesoris MG-1) in subgingival plaque. Rat bone marrow mesenchymal stem cells (BMSCs) were cultured on the surface of two kinds of antibacterial nano-netting titanium materials to analyze their osteogenic differentiation activity. The surface carbon pollution was removed effectively, and the loaded nano-silver ion nanowire layer titanium material. 2 ~ (2 +) UV was successfully prepared by immersing in silver nitrate solution to UV irradiation and reducing, and effectively resisting the early attachment of Actinomycesoris MG-1 to the nano-netted titanium material treated by UV. Moreover, the formation of biofilm was inhibited for 6 hours. The silver loaded nanofilm layer titanium not only killed the attached bacteria, but also inhibited the formation of biofilm. The results showed that the long-term antibacterial activity was 28 days. 4 days after treatment with nanowet layer titanium, the early attachment and proliferation of rat bone marrow mesenchymal stem cells (BMSCs) and ALP activity, osteocalcin secretion and extracellular matrix mineralization were promoted. The expression level of osteoblast-associated gene (ALP) Runx 2 (BMPN) was significantly higher than that of nano-mesh layer titanium (0.05), and that of silver-loaded nano-mesh layer titanium was higher than that of nano-netted titanium material. The inhibition of bone marrow mesenchymal stem cells (BMSCs) in the early stage of attachment and proliferation and the activity of ALP. Conclusion the treatment of nanometer-net layer titanium can inhibit the activity of bone marrow mesenchymal stem cells for more than 6 hours and promote the differentiation of bone marrow mesenchymal stem cells. In this study, the biocompatibility of silver-loaded nanometer-net layer titanium material was further studied, which maintained antibacterial activity for 28 days, but had a certain degree of cytotoxicity.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.1

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本文編號：1505033