本文關(guān)鍵詞： 礦物質(zhì) 膠原 組織支架 細(xì)胞黏附 骨再生　出處：《北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2014年01期 　論文類型：期刊論文

【摘要】：目的:研究?jī)煞N礦化膠原的顯微結(jié)構(gòu)對(duì)人成骨樣細(xì)胞MG 63的黏附力和形貌的影響。方法:采用傳統(tǒng)礦化法和生物仿生礦化法分別制備纖維外礦化膠原(extrafibrillarly-mineralized collagen,EMC)與纖維內(nèi)礦化膠原(intrafibrillarly-mineralized collagen,IMC)支架材料,人成骨樣細(xì)胞MG 63作為實(shí)驗(yàn)用細(xì)胞,用掃描電子顯微鏡(scanning electron microscopy,SEM)檢測(cè)兩種支架材料的顯微結(jié)構(gòu)及細(xì)胞與材料間相互作用。采用激光共聚焦顯微鏡(laser scanning microscope,LSM)檢測(cè)接種在材料上細(xì)胞的黏附力與形貌。結(jié)果:不同礦化方式影響膠原的顯微結(jié)構(gòu)。EMC組可見花樣團(tuán)簇狀的晶體無規(guī)則沉積在納米纖維束表面,而IMC組纖維束表面光滑,無晶體沉積。能譜分析結(jié)果顯示其內(nèi)部含有晶體,為含碳的鈣缺乏型羥基磷灰石,這與天然骨礦化膠原成分類似。LSM結(jié)果顯示,IMC比EMC支架材料更能促進(jìn)MG 63細(xì)胞伸展。熒光定量分析結(jié)果表明MG 63細(xì)胞對(duì)IMC支架材料的黏附力(18.54±2.71)明顯高于EMC支架材料(14.29±1.32)。SEM結(jié)果顯示兩種礦化膠原均具有良好的生物相容性,細(xì)胞在不同支架上呈現(xiàn)不同的形貌。結(jié)論:礦化膠原的顯微結(jié)構(gòu)影響細(xì)胞的形貌,IMC支架材料更能促進(jìn)MG63細(xì)胞的黏附與伸展,這將有助于研制新的牙槽骨再生的生物仿生材料。
[Abstract]:Objective: to study the effect of microstructures of two kinds of mineralized collagen on adhesion and morphology of human osteoblast-like cell MG63. Methods: extracellular mineralized collagen (EMCMC) and fibers were prepared by traditional mineralization method and biomimetic mineralization method, respectively. Intrafibrillarly-collagenized IMCC-based scaffold material, Human osteoblast-like cell MG63 was used as experimental cell. Scanning electron microscopy (SEM) was used to detect the microstructure of the two scaffolds and the interaction between cells and materials. The adhesion and morphology of the cells inoculated on the materials were detected by laser confocal microscopy (LSM). The microstructures of collagen were affected by different mineralization modes. In EMC group, irregular crystals were deposited on the surface of nanofilament bundles. In IMC group, the surface of fiber bundle is smooth and there is no crystal deposition. The results of energy spectrum analysis show that the fiber bundle contains crystal, which is calcium deficient hydroxyapatite. The results of fluorescence quantitative analysis showed that the adhesion of MG63 cells to IMC scaffolds was significantly higher than that of EMC scaffolds, and the fluorescence quantitative analysis showed that the adhesion of MG63 cells to IMC scaffolds was significantly higher than that of EMC scaffolds. The results of SEM showed that the two mineralized collagen had good biocompatibility. Conclusion: the microstructure of mineralized collagen can affect the morphology of MG63 cells, which can promote the adhesion and extension of MG63 cells, which will be helpful for the development of new biomimetic materials for alveolar bone regeneration.
【作者單位】： 北京大學(xué)口腔醫(yī)學(xué)院·口腔醫(yī)院正畸科;
【基金】：國(guó)家自然科學(xué)基金(81201198)資助~~
【分類號(hào)】：R783.1

【共引文獻(xiàn)】

相關(guān)博士學(xué)位論文 前6條

1 宿廣昊;取向膠原基材料的制備及影響因素的考察[D];北京協(xié)和醫(yī)學(xué)院;2012年

2 林y，

本文編號(hào)：1500463