本文關(guān)鍵詞： 口底鱗癌 免疫組織化學(xué) WAVE1 RAC1 CDK8　出處：《吉林大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：研究背景：口腔癌是一種常見的粘膜上皮性腫瘤，約占全身惡性腫瘤的3.5%，其發(fā)病率及死亡率不容忽視。其中發(fā)生于口腔粘膜的鱗狀細(xì)胞癌約占口腔惡性腫瘤的90%。鱗狀細(xì)胞癌是一種主要發(fā)生于黏膜、皮膚或附屬器的上皮性惡性腫瘤，其惡性程度高，，轉(zhuǎn)移率高，發(fā)病率高。因此，鱗狀細(xì)胞癌成為醫(yī)學(xué)領(lǐng)域亟待解決的難題。從基因水平研究腫瘤已成為研究者競相追逐的焦點(diǎn)。近年來研究表明，全身惡性腫瘤的發(fā)生發(fā)展均與WAVE1、RAC1和CDK8基因的異常表達(dá)有關(guān)。因此，WAVE1、RAC1和CDK8基因越來越受到醫(yī)學(xué)研究者的關(guān)注，成為目前研究腫瘤的新熱點(diǎn)。 目的:觀察在WAVE1、RAC1和CDK8口底鱗癌中的表達(dá)情況，探討WAVE1、RAC1和CDK8在口底鱗癌治療中和預(yù)后的意義。 方法:選擇口腔鱗癌標(biāo)本83例和43例正�？谇火つ閷φ战M。采用免疫組化SuperPicTureTMPolyme法檢測WAVE1，RAC1和CDK8的表達(dá)。并對口底癌組織WAVE1、RAC1和CDK8的表達(dá)進(jìn)行相關(guān)性分析。 結(jié)果: 1. WAVE1、RAC1和CDK8在口底癌中的表達(dá)均高于癌旁組織的表達(dá)，差異有統(tǒng)計學(xué)意義。（P＜0.05）。SP相關(guān)性分析顯示，WAVE1，RAC1、CDK8的表達(dá)成正相關(guān)（P＜0.05）。 2. WAVE1的表達(dá)與口底癌的組織分化及淋巴轉(zhuǎn)移相關(guān)（P＜0.05），與年齡及臨床分期無關(guān)（p＞0.05）。RAC1的表達(dá)與口底癌的組織分化、淋巴轉(zhuǎn)移相關(guān)（P＜0.05），與臨床分期及年齡無關(guān)（p＞0.05）。CDK8的表達(dá)與口底癌的組織分化及淋巴轉(zhuǎn)移相關(guān)（P＜0.05），與年齡及臨床分期無關(guān)（p＞0.05）。 結(jié)論: WAVE1，RAC1和CDK8在口底癌中呈現(xiàn)高表達(dá)。早期篩查WAVE1、 RAC1和CDK8指標(biāo)對于口底鱗狀細(xì)胞癌病情演變和預(yù)后評估有重要臨床意義。
[Abstract]:Background: oral cancer is a common mucosal epithelial tumor. The incidence and mortality rate of malignant tumors of the whole body can not be ignored. The squamous cell carcinoma occurring in the oral mucosa accounts for about 90% of the malignant tumor of the mouth. Squamous cell carcinoma is a kind of mainly occurring in the mucous membrane. Epithelial malignant neoplasms of the skin or appendages with high malignancy, high metastatic rate, and high incidence. Squamous cell carcinoma (SCC) has become an urgent problem in the medical field. The study of cancer at the gene level has become a hot topic in recent years. The occurrence and development of malignant tumors of the whole body are related to the abnormal expression of RAC1 and CDK8 genes in WAVE1. therefore, the RAC1 and CDK8 genes of WAVE1 have been paid more and more attention by medical researchers and become a new focus of tumor research. Objective: to observe the expression of WAVE1pRAC1 and CDK8 in squamous cell carcinoma of the oral floor, and to explore the significance of WAVE1-RAC1 and CDK8 in the treatment and prognosis of squamous cell carcinoma of the floor of mouth. Methods: 83 cases of oral squamous cell carcinoma (OSCC) and 43 cases of normal oral mucosa were selected as control group. The expressions of WAVE1pRAC1 and CDK8 were detected by immunohistochemical SuperPicTureTMPolyme method, and the correlation between WAVE1pRAC1 and CDK8 was analyzed. Results:. 1. The expressions of RAC1 and CDK8 in the carcinoma of floor of mouth were higher than those in the adjacent tissues, and the correlation analysis showed that the expression of CDK8 was positively correlated with the expression of RAC1 and CDK8 in the tumor tissues (P < 0.05, P < 0.05, P < 0.05, P < 0.05). 2. The expression of WAVE1 was correlated with tissue differentiation and lymphatic metastasis of fundus oral carcinoma (P < 0.05), but not with age and clinical stage (P > 0.05). The expression of CDK8 was not correlated with the clinical stage and age, and the expression of CDK8 was not correlated with the histological differentiation and lymphatic metastasis of basal oral carcinoma (P < 0.05), but had no correlation with age and clinical stage (p > 0.05). Conclusion: high expression of WAVE1 / RAC1 and CDK8 is present in basal oral carcinoma, and early screening of WAVE1 / RAC1 and CDK8 is of great clinical significance in evaluating the prognosis and progression of squamous cell carcinoma of the floor of mouth.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R739.8

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