本文關(guān)鍵詞：咬肌力對大鼠下頜骨及髁突生長發(fā)育的影響　出處：《南昌大學》2015年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 曲安奈德 髁突 下頜骨 咬肌 IGF-Ⅰ

【摘要】：目的:通過兩種不同的方法處理咬肌,建立大鼠咬肌力減弱及咬肌力增強的動物模型,并觀察這兩種方法對大鼠髁突及下頜骨生長的影響。方法:48只21日齡SD大鼠,隨機分為3組,每組16只,于室內(nèi)溫度22℃、相對濕度為50%~60%、氣流速度≤0.2、氨濃度≤14mg/m3、噪聲≤60dB條件下喂養(yǎng)一周后,進行試驗。A組咬肌力增強組:大鼠咀嚼巧克力浸泡的木塊,以增強大鼠咬肌力量;B組咬肌力減弱組:采用醋酸曲安奈德0.01ml/100g處理大鼠咬肌,以使大鼠咬肌力減弱;C組對照組,不作任何處理。在建模第3周,第6周后每組分別處死大鼠8只,分別行下頜骨形態(tài)學測量及髁突組織學檢測,并進行胰島素樣生長因子-Ⅰ(Insulin like growth factor-Ⅰ,IGF-Ⅰ)表達含量的檢測,比較其在不同組及同組不同時間點表達的變化。結(jié)果:本實驗成功建立了大鼠咬肌力增強及咬肌力減弱的實驗模型。⑴下頜骨形態(tài)學測量顯示:①:下頜骨長度測量結(jié)果顯示:B組(咬肌力減弱組)大鼠下頜骨長度明顯小于A組(咬肌力增強組)大鼠下頜骨長度;C組(對照組)大鼠下頜骨長度明顯小于A組(咬肌力增強組)大鼠下頜骨長度;C組(對照組)大鼠下頜骨長度大于B組(咬肌力減弱組)大鼠下頜骨長度,三者兩兩比較,差別有統(tǒng)計學意義(p0.05)。②:下頜升支高度測量結(jié)果顯示:A組大鼠下頜支高度大于B組與C組大鼠下頜支高度;C組大鼠下頜支高度要明顯大于B組大鼠下頜支高度,差別有統(tǒng)計學意義(p0.05)。③:下頜角角度測量結(jié)果顯示:A組大鼠下頜角度大于C組大鼠下頜角度,C組大鼠下頜角度大于B組大鼠下頜角度,差別有統(tǒng)計學意義(p0.05)。④:下頜體高度測量結(jié)果顯示:A組,B組,C組兩兩比較,差別無統(tǒng)計學意義(p0.05)。⑵髁突組織學檢測結(jié)果顯示:①:咬肌力增強組大鼠髁突軟骨各細胞層分層明顯,細胞排列密集;②:咬肌力減弱組大鼠髁突軟骨各細胞層細胞排列稀疏;③:對照組髁突軟骨各細胞層排列稀疏,相對于咬肌力減弱組而言,細胞排列較密集。⑶髁突軟骨免疫組化結(jié)果顯示,IGF-Ⅰ主要表達于成軟骨細胞層和肥大細胞層。IGF-1蛋白半定量結(jié)果顯示:IGF-1因子在髁突軟骨細胞中的表達率,增殖層,成軟骨細胞層,肥大層陽性細胞表達術(shù)后三周要強于術(shù)后六周;IGF-1因子在增殖層的表達表現(xiàn)為同一時刻A、B、C三組表達有差異,咬肌力增強組的表達強于對照組,對照組強于咬肌力減弱組。結(jié)論:1:在本實驗環(huán)境條件下,咬肌力大時,有利于髁突及下頜骨的生長,并使下頜角角度減小。咬肌力減弱時,下頜角角度增大。2:在本實驗環(huán)境條件下,髁突軟骨各細胞層細胞的分化與壓力的大小呈正相關(guān)。3:IGF-Ⅰ因子作為一種生長因子,在生長發(fā)育期間,應力越大,IGF-Ⅰ因子陽性表達率越高,IGF-Ⅰ因子可以作為檢測髁突生長發(fā)育的指標。
[Abstract]:Objective: through two different methods of processing the masseter muscle, muscle weakness and a bite bite strength of the animal model of rats, and observe the effects of the two methods on the growth of condyle and mandible in rats. Methods: 48 21 day old SD rats were randomly divided into 3 groups, 16 rats in each group, in the room temperature of 22 degrees Celsius, relative humidity is 50%~60%, flow rate is less than 0.2, the ammonia concentration is less than or equal to 14mg/m3, a week after the noise is less than or equal to 60dB under the condition of feeding, test group.A bite strength group: rats were chewing chocolate soaked wood, in order to enhance the strength of masseter muscle weakness; group B group: bite by acetic acid 0.01ml/100g triamcinolone treated rats masseter muscle, to make rat bite muscle weakness; group C control group, without any treatment. In third weeks, sixth weeks after modeling, rats in each group were sacrificed at 8, respectively, the Mandible Condyle morphology measurement and histological examination, and insulin-like growth factor-I (Insu Lin like growth factor- I, IGF- I) expression were detected, comparing the changes in different groups and different time points in group expression. Results: we have successfully established experimental model of rat bite bite strength and muscle weakness. The mandibular morphology measurement showed: 1: the measurement results showed that the length of mandible B group (bite muscle weakness group) rat mandibular length is less than the A group (bite strength group) rat mandibular length; C group (control group) the length of mandible in rats were significantly less than group A (bite strength group) rat mandibular length; C group (control group) of rat mandibular length is greater than the B group (bite muscle weakness group) rat mandibular length, 22 compared with three, the difference was statistically significant (P0.05). The mandibular ramus height measurement results showed that A group rats mandibular ramus height greater than B group and C group rat mandibular ramus height; C rats mandibular ramus height to Ming Rats in B group were significantly larger than the height of the mandibular ramus, the difference was statistically significant (P0.05). The mandibular angle measurement results showed that: A rats mandibular angle is greater than the C rats C rats mandibular angle, mandibular angle is greater than the group B rat mandibular angle, the difference was statistically significant (P0.05). 4. The results show: mandibular height measurement: A group, B group, C group 22, the difference was not statistically significant (P0.05). The condylar histological examination of the results showed that: bite strength in condylar cartilage of rats in all cell layers increased, cells arranged densely; the bite muscle weakness of condyle cartilage of rats at each cell layer cells are sparse; the control group: the condylar cartilage layers are sparse, with respect to bite muscle weakness group, cells arranged indense. The condylar cartilage immunohistochemistry results showed that IGF- I mainly expressed in chondrogenic cell layer and the layer of.IGF-1 protein half mast cells The quantitative results showed that the expression of factor IGF-1 in condylar cartilage cells proliferation rate, layer, chondroblast layer, hypertrophic layer positive cells after three weeks than 6 weeks after surgery; IGF-1 factor showed the same time A expression in proliferative layer B, C three groups with different expression, bite strength enhanced expression of strong group than in the control group, the control group is stronger than the bite muscle weakness group. Conclusion: 1: under the experimental conditions, bite strength, is conducive to the growth of condyle and mandible, and the mandibular angle decreases. Bite muscle weakness, mandibular angle increased.2: in this experimental environment under the size of.3:IGF- was positively related to differentiation factor and pressure of condylar cartilage layers of cells as a growth factor in the growth period, the greater the stress factor, IGF- positive expression rate is high, the IGF- factor can be used to measure the condylar growth index.

【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R783.5

【參考文獻】

相關(guān)期刊論文 前10條

1 李永清;;糖皮質(zhì)激素的藥理作用及不良反應[J];北方藥學;2013年11期

2 李玲玲;倪滔;李松;羅應偉;;不對稱咀嚼力作用下CTGF在髁突軟骨中的表達[J];昆明醫(yī)科大學學報;2013年10期

3 江莉婷;魏立;謝銀銀;周琦;朱雅萍;高益鳴;;IGF-1信號通路相關(guān)蛋白在大鼠髁突軟骨細胞增殖分化中的表達[J];口腔頜面外科雜志;2013年03期

4 周琦;江莉婷;魏立;朱雅萍;高益鳴;;IGF-1、IGF-1R及Bcl-2,Bax在大鼠髁突軟骨發(fā)育中的表達及作用[J];現(xiàn)代口腔醫(yī)學雜志;2013年01期

5 常世民;柳春明;姚玉勝;;肌內(nèi)注射曲安奈德誘導犬咬肌萎縮的實驗研究[J];北京口腔醫(yī)學;2009年02期

6 劉玉生;柳大烈;杜本軍;鄭健生;;曲安奈德肌內(nèi)注射誘導兔咬肌萎縮的實驗研究[J];中國新藥雜志;2006年23期

7 馮傳波;高建華;劉紅梅;;肉毒桿菌毒素A注射治療良性咬肌肥大[J];中國臨床康復;2006年36期

8 杜本軍;柳大烈;蘇冰;梁莉;劉玉生;鄭鍵生;;選擇性神經(jīng)離斷咬肌神經(jīng)動物模型的建立[J];第四軍醫(yī)大學學報;2006年10期

9 柳大烈;劉玉生;杜本軍;鄭健生;;糖皮質(zhì)激素誘導兔咬肌萎縮的實驗[J];中國臨床康復;2006年08期

10 劉剛,胡蘊玉,韓一生;IGF_1對體外兔關(guān)節(jié)軟骨細胞的生物學效應[J];第四軍醫(yī)大學學報;2002年08期

相關(guān)博士學位論文 前2條

1 黃進軍;咬肌神經(jīng)切除和化學去神經(jīng)對大鼠下頜骨生長發(fā)育影響的實驗研究[D];南方醫(yī)科大學;2011年

2 劉玉生;肌內(nèi)注射曲安奈德及肉毒毒素誘導兔咬肌萎縮試驗及臨床研究[D];第一軍醫(yī)大學;2006年

相關(guān)碩士學位論文 前2條

1 李玲玲;單側(cè)顳肌切除、咬肌阻斷對下頜骨生長發(fā)育影響的研究[D];昆明醫(yī)學院;2010年

2 蘭柳萍;不同咀嚼力對SD大鼠下頜骨髁突生長發(fā)育影響的實驗研究[D];昆明醫(yī)學院;2009年

，

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