【摘要】：目的:探討免疫及炎癥反應與精神分裂癥的關系。方法:①根據(jù)國際疾病和相關健康問題統(tǒng)計分類第十版(ICD-10)選取從2016年3月至2016年12月在延邊腦科醫(yī)院住院的診斷為精神分裂癥的患者,共收集84例,其中男性43例、女性41例。另選52例年齡相當?shù)捏w檢健康者為健康對照組,其中男性24例、女性28例。②用陽性與陰性癥狀量表(PANSS)評定精神分裂癥患者精神癥狀。③搜集患者年齡、性別、婚姻狀況、身高及體重等一般資料,計算BMI值。④取清晨空腹血(禁食12小時),采用全自動生化分析儀檢測同型半胱氨酸(HCY)、C反應蛋白(CRP);用全自動血細胞分析儀及配套試劑盒質控品分類檢驗白細胞(WBC)、紅細胞(RBC)、血紅蛋白(HGB)、血細胞比容(HCT)、血小板(PLT)、中性粒細胞百分比(NEU%)、淋巴細胞百分比(LYM%)、中性粒細胞(NEU)、淋巴細胞(LYM)等,并計算中性粒細胞-淋巴細胞比率(NLR);用酶聯(lián)免疫吸附法(ELISA)測定白細胞介素-6(IL-6)濃度。⑤采用SPSS 20.0軟件進行統(tǒng)計學分析。正態(tài)分布的計量資料以均數(shù)±標準差(x±SD)表示,進行獨立樣本的t檢驗,非正態(tài)分布的計量資料以中位數(shù)(四分位數(shù)間距)[M(P25,P75)]表示,進行Mann-WhitneyU檢驗;計數(shù)數(shù)據(jù)以百分率表示,進行χ2檢驗;IL-6與其相關因素的相關性采用Spearman相關分析,免疫和炎癥因子對精神分裂癥的影響采用多因素logistic回歸分析,以P0.05為差異有統(tǒng)計學意義。結果:①精神分裂癥組 NLR、HCY、IL-6 和 LYMPH%分別為 2.06±0.80、17.1(8.65,28.53)、61.42(21.42,111.96)、和 31.22±6.88,健康對照組分別為 1.71±0.57、11.20(8.03,15.98)、25.61(14.34,66.12)和 35.58±7.83,其差異有統(tǒng)計學意義(P值均0.05);精神分裂癥男性組NLR、IL-6和LYMPH%分別為2.01±0.83、61.96(19.35,128.01)和 31.56±7.42,健康對照男性組分別為 1.59±0.54、31.10(17.67,66.12)和37.13±8.04,其差異有統(tǒng)計學意義(P值均0.05);精神分裂癥女性組 NLR、IL-6 和 LYMPH%分別為 2.11±0.77、62.22(21.95,107.19)和30.86±6.34,健康對照女性組分別為 1.82±0.55、25.49(14.34,50.66)和 34.26±7.52,其差異有統(tǒng)計學意義(P值均0.05)。②精神分裂癥患者HCY與IL-6呈正相關關系,r值為0.423,其相關性有統(tǒng)計學意義(P0.001)。③精神分裂癥患者IL-6與PANSS總分和陰性癥狀均呈正相關關系,r值分別為0.777和0.861,其相關性均有統(tǒng)計學意義(P值均0.001)。④多因素logistic回歸分析結果顯示,精神分裂癥與IL-6和NLR均密切相關,其 OR 值分別為 1.039(95%CI:1.000～1.014,P0.05)和 1.706(95%CI:1.146～3.869,P0.05)。結論:精神分裂癥患者的NLR、IL-6、HCY高于健康者,其中HCY與IL-6呈正相關,其可作為炎癥因子在實驗室檢查或者化驗結果支持臨床進行診斷,作為診斷依據(jù)。IL-6濃度與與精神分裂癥PANSS總分及陰性癥狀分呈正相關,說明其可作為輔助評價精神分裂癥病情嚴重程度及陰性癥狀的一個生物標記物。NLR、IL-6為精神分裂癥患病的影響因素�？梢宰鳛榫穹至寻Y患病的預測因子,提高精神分裂癥診斷的準確性。
[Abstract]:Objective: to investigate the relationship between immunity and inflammatory response and schizophrenia. Methods: 1 according to the tenth edition of the International Statistical Classification of Diseases and related Health problems (ICD-10), 84 patients with schizophrenia diagnosed in Yanbian brain Hospital from March 2016 to December 2016 were collected, including 43 males. 41 cases were female. Another 52 healthy persons of the same age were selected as the healthy control group, including 24 males and 28 females. 2 the mental symptoms of schizophrenia patients were evaluated by positive and negative symptoms scale (PANSS). 3 the age, sex and marital status of the patients were collected. The BMI value was calculated according to the general data such as height and weight. 4 fasting blood was taken in the morning (fasting for 12 hours) and homocysteine (HCY), C reactive protein (CRP); was detected by automatic biochemical analyzer. The percentage of white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), blood cell specific volume (HCT), platelet (PLT), neutrophil (NEU%) was measured by automatic blood cell analyzer and matching kit quality control. The percentage of lymphocytes (LYM%), neutrophils (NEU), lymphocytes (LYM), etc., and the neutrophil-lymphocytes ratio (NLR); was calculated. The concentration of IL-6 (IL-6) was determined by enzyme-linked immunosorbent assay (ELISA). 5 the concentration of IL-6 was statistically analyzed by SPSS 20.0 software. The measurement data of normal distribution are represented by mean 鹵standard deviation (x 鹵SD). T test of independent samples is carried out, and the measurement data of non-normal distribution are represented by median (quartile spacing) [M (P25, P75)]. Mann-WhitneyU test is carried out. The counting data were expressed as percentage, and the 蠂 2 test was carried out. The correlation between IL-6 and its related factors was analyzed by Spearman correlation analysis, and the influence of immune and inflammatory factors on schizophrenia was analyzed by multivariate logistic regression analysis, and the difference was statistically significant (P 0.05). Results: 1in schizophrenia group, NLR,HCY,IL-6 and LYMPH% were 2.06 鹵0.80,17.1 (8.65, 28.53), 61.42 (21.42111.96) and 31.22 鹵6.88, respectively. The healthy control group was 1.71 鹵0.57, 11.20 (8.03, 15.98), 25.61 (14.34, 66.12) and 35.58 鹵7.83, respectively, the difference was statistically significant (P 0.05). NLR,IL-6 and LYMPH% in schizophrenia male group were 2.01 鹵0.83, 61.96 (19.35128.01) and 31.56 鹵7.42, respectively, and those in healthy control male group were 1.59 鹵0.54, 31.10 (17.67, 66.12) and 37.13 鹵8.04, respectively. The difference was statistically significant (P 0.05). NLR,IL-6 and LYMPH% in schizophrenia group were 2.11 鹵0.77, 62.22 (21.95107.19) and 30.86 鹵6.34, respectively, while those in healthy control group were 1.82 鹵0.55, 25.49 (14.34, 50.66) and 34.26 鹵7.52, respectively. The difference was statistically significant (P < 0.05). 2 there was a positive correlation between HCY and IL-6 in patients with schizophrenia (r = 0.423, P < 0.05). The correlation was statistically significant (P0.001). 3 IL-6 was positively correlated with the total score and negative symptoms of PANSS in patients with schizophrenia, r values were 0.777 and 0.861, respectively. The correlation was statistically significant (P = 0.001). 4 the results of multivariate logistic regression analysis showed that schizophrenia was closely related to IL-6 and NLR, and the OR values were 1.039 (95% CI 鈮，

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