【摘要】：目的研究慢性不可預(yù)知溫和應(yīng)激(UCMS)對SAMP8小鼠抑郁情緒、學(xué)習(xí)記憶能力的影響,及UCMS對海馬中腦源性神經(jīng)營養(yǎng)因子(BDNF)及其受體(Trk B)、神經(jīng)元凋亡相關(guān)因子B淋巴細(xì)胞瘤-2基因(Bcl-2)、Bcl-2相關(guān)X蛋白(Bax)蛋白表達(dá)的影響。方法選取健康、7月齡、雄性SAMP8小鼠20只,對其進(jìn)行隨機(jī)分組,分為應(yīng)激組和非應(yīng)激組,每組各10只。然后對兩組小鼠進(jìn)行為期21天的慢性不可預(yù)知應(yīng)激,應(yīng)激完成后再進(jìn)行水迷宮實(shí)驗(yàn)及糖水偏好試驗(yàn)。行為學(xué)實(shí)驗(yàn)完成后,應(yīng)激組和非應(yīng)激組各隨機(jī)選取6只小鼠進(jìn)行斷頭取海馬,采用蛋白質(zhì)印跡法(westernblotting)對BDNF及Trk B、Bcl-2、Bax的蛋白表達(dá)量進(jìn)行測定。結(jié)果1經(jīng)過5天的訓(xùn)練后,應(yīng)激組小鼠尋找到平臺(tái)的潛伏期明顯增長(P0.05);在第6天撤除平臺(tái)后,應(yīng)激組小鼠穿越平臺(tái)次數(shù)比非應(yīng)激組明顯減少(P0.05)。2應(yīng)激后應(yīng)激組小鼠比非應(yīng)激組糖水消耗量明顯下降(P0.05)。3與非應(yīng)激組比較,應(yīng)激組小鼠BDNF及Trk B蛋白表達(dá)明顯減少,且具有統(tǒng)計(jì)學(xué)意義(P0.05)。4與非應(yīng)激組比較,應(yīng)激組小鼠海馬Bcl-2蛋白表達(dá)明顯減少,Bax蛋白表達(dá)明顯增多,且具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論應(yīng)激使SAMP8小鼠產(chǎn)生抑郁情緒、學(xué)習(xí)記憶能力下降;應(yīng)激導(dǎo)致的BDNF及其Trk B、Bcl-2蛋白表達(dá)量減少,Bax蛋白量表達(dá)增多。
[Abstract]:Objective to study the effects of chronic unpredictable mild stress (UCMS) on depression, learning and memory ability in SAMP8 mice, and the effect of UCMS on brain-derived neurotrophic factor (BDNF) and its receptor (Trk B), in hippocampus. Effect of apoptosis-related factor B lymphocyte tumor-2 gene (Bcl-2) and Bcl-2-associated X protein (Bax) protein expression. Methods Twenty healthy 7-month-old male SAMP8 mice were randomly divided into two groups: stress group (n = 10) and non-stress group (n = 10). Two groups of mice were subjected to chronic unpredictable stress for 21 days. After the stress was completed, the water maze test and sugar-water preference test were performed. After the behavioral experiment was completed, six mice in the stress group and the non-stress group were randomly selected and the hippocampus was decapitated. The protein expression of BDNF, Trk-B, Bcl-2 and Bax were measured by protein blot (westernblotting). Results (1) after 5 days of training, the latency of finding the platform in the stress group increased significantly (P0.05). After removing the platform on the 6th day, the number of crossing the platform in the stress group was significantly lower than that in the non-stress group (P0.05). 2 the consumption of sugar water in the stress group was significantly lower than that in the non-stress group (P0.05). 3Compared with the non-stress group, the frequency of crossing the platform in the stress group decreased significantly (P0.05). (4) compared with non-stress group, the expression of BDNF and Trk B protein in hippocampus of stress group decreased significantly, and the expression of Bax protein increased significantly, compared with non-stress group, the expression of Bcl-2 and Bax protein in hippocampus of stress group was significantly decreased, and the expression of Bax protein was significantly higher than that of non-stress group. And has statistical significance (P0.05). Conclusion stress induced depression and decreased the ability of learning and memory in SAMP8 mice, the expression of Bcl-2 protein and Bcl-2 protein decreased and the expression of Bax protein increased in BDNF and Trk-B induced by stress.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R749.4

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