【摘要】：目的：探討不同程度的慢性酒精中毒對大鼠內(nèi)耳肽類激素VIP(血管活性腸肽)及其受體VIPR、SP（P物質(zhì)）及其受體（SPR）表達的影響及其機制。 方法：30只Sprague Dawley (SD)大鼠隨機分為三組（輕度酒精中毒組、重度中毒組及對照組）進行造模。采用RT-PCR分別檢測三組大鼠內(nèi)耳VIP的表達，采用western blot檢測大鼠內(nèi)耳VIP受體蛋白的表達，采用免疫組化的方法定位及定量檢測大鼠內(nèi)耳VIP的表達。 結(jié)果：慢性酒精中毒組的大鼠內(nèi)耳VIP及其受體的表達下調(diào)，并且重度慢性酒精中毒的大鼠內(nèi)耳VIP及其受體的表達低于輕度組，差異有統(tǒng)計學(xué)意義（P0.05）。慢性酒精中毒組的大鼠內(nèi)耳VIP及其受體與對照組在神經(jīng)節(jié)細胞的表達差異無統(tǒng)計學(xué)意義（P0.05）。 結(jié)論： VIP及其受體、SP及其受體在慢性酒精中毒大鼠的聽覺系統(tǒng)功能中發(fā)揮著重要的作用。慢性酒精中毒可引起聽覺系統(tǒng)的一種肽類激素分泌不平衡，，最終導(dǎo)致聽力損失。
[Abstract]:Aim: to investigate the effect and mechanism of chronic alcoholism on the expression of vasoactive intestinal peptide (VIP) and its receptor VIPR,SP (substance P) and its receptor (SPR) in rat inner ear. Methods: thirty Sprague Dawley (SD) rats were randomly divided into three groups: mild alcoholism group, severe intoxication group and control group. The expression of VIP in the inner ear of the three groups was detected by RT-PCR, the expression of VIP receptor protein in the inner ear of the rat was detected by western blot, and the expression of VIP in the inner ear of the rat was detected quantitatively by immunohistochemical method. Results: the expression of VIP and its receptor in the inner ear of the rats with chronic alcoholism was down-regulated, and the expression of VIP and its receptor in the inner ear of the rats with severe chronic alcoholism was lower than that of the mild group (P0.05). There was no significant difference in the expression of VIP and its receptor between the chronic alcoholism group and the control group in ganglion cells (P0.05). Conclusion: VIP and its receptors, SP and their receptors play an important role in the auditory system function of chronic alcoholism rats. Chronic alcoholism can cause an imbalance in the secretion of peptide hormones in the auditory system, leading to hearing loss.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R749.62

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