【摘要】：為了探討大麻素受體1基因(Cannabinoid receptor 1,CNR1)3個(gè)SNP位點(diǎn)(rs6454674、rs1049353和rs806368)、谷氨酸脫羧酶1基因(Glutamate decarboxylase 1,GAD1)3個(gè)SNP位點(diǎn)(rs1978340、rs3791878和rs11542313)、腦源性神經(jīng)營養(yǎng)因子基因(Brain-derived neurotrophic factor,BDNF)2個(gè)SNP位點(diǎn)(rs6265和rs13306221)與云南傣族男性海洛因依賴的相關(guān)性,文章采用病例對(duì)照關(guān)聯(lián)分析,建立8-SNPs復(fù)合擴(kuò)增體系,應(yīng)用SNaPshot方法檢測(cè)165例傣族男性海洛因依賴患者和170例健康傣族男性CNR1、GAD1和BDNF基因8個(gè)SNPs位點(diǎn)基因型,采用SPSS17.0、Haploview4.2、PHASE2.1和MDR軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果顯示,rs13306221基因型頻率在海洛因依賴組和對(duì)照組中的差異具有統(tǒng)計(jì)學(xué)意義(P0.025),海洛因依賴組rs6265 A等位基因顯著高于對(duì)照組(P0.05),由rs1978340-rs3791878-rs11542313構(gòu)建的T-A-C、C-C-C、C-C-T和T-C-C單體型及rs6265-rs13306221構(gòu)建的A-G單體型頻率在海洛因依賴組及對(duì)照組中差異具有統(tǒng)計(jì)學(xué)意義(P0.05),海洛因依賴組T-A-C、C-C-T和A-G單體型頻率明顯高于對(duì)照組,GAD1基因rs1978340與rs3791878之間可能存在強(qiáng)協(xié)同的交互作用。結(jié)果表明,CNR1基因rs1049353多態(tài)性、GAD1基因rs1978340和rs11542313多態(tài)性以及BDNF基因rs6265和rs13306221多態(tài)性與云南傣族男性海洛因依賴相關(guān)聯(lián),并且攜帶rs6265 A等位基因的個(gè)體可能更容易對(duì)海洛因產(chǎn)生依賴。
[Abstract]:To investigate three SNP loci (rs6454674,rs1049353 and rs806368) and three SNP loci (rs1978340,rs3791878 and rs11542313) of cannabinoid receptor 1 gene (Cannabinoid receptor 1 CNR1 and glutamate decarboxylase 1 gene (Glutamate decarboxylase 1 GAD1, respectively. The relationship between two SNP loci (rs6265 and rs13306221) of brain-derived neurotrophic factor (Brain-derived neurotrophic factor,BDNF) gene and heroin dependence in Dai males in Yunnan Province was studied. A 8-SNPs multiplex amplification system was established by case-control analysis. The genotypes of 8 SNPs loci of CNR1,GAD1 and BDNF gene in 165 Dai male heroin addicts and 170 healthy Dai men were detected by SNaPshot method. The results were statistically analyzed by SPSS17.0,Haploview4.2,PHASE2.1 and MDR software. The results showed that the frequency of rs13306221 genotype was significantly different between heroin dependence group and control group (P0.025). The allele of rs6265 A in heroin dependence group was significantly higher than that in control group (P0.05). The frequencies of T-A-CnC-C-T and T-C-C haplotype constructed by rs1978340-rs3791878-rs11542313 and A-G haplotype constructed by rs6265-rs13306221 were significantly different between heroin dependence group and control group (P0.05). The frequencies of T-A-CnC-C-T and A-G haplotypes in heroin dependence group were significantly higher than those in control group. There might be a strong synergistic interaction between rs1978340 and rs3791878 of GAD1 gene. The results showed that rs1049353 polymorphism of CNR1 gene, rs1978340 and rs11542313 polymorphism of GAD1 gene and rs6265 and rs13306221 polymorphism of BDNF gene were associated with heroin dependence in Dai males in Yunnan, and individuals with rs6265 A allele might be more susceptible to heroin dependence.
【作者單位】： 昆明醫(yī)科大學(xué)法醫(yī)學(xué)院;云南省公安廳刑事科學(xué)技術(shù)研究所 公安部毒品分析及禁毒技術(shù)重點(diǎn)實(shí)驗(yàn)室;西雙版納州公安局;
【基金】：公安部科技強(qiáng)警基礎(chǔ)工作專項(xiàng)項(xiàng)目(編號(hào):2013GABJC017)資助
【分類號(hào)】：R749.61

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