青春期認(rèn)知功能干預(yù)對(duì)成年期甲基苯丙胺覓藥行為的影響
發(fā)布時(shí)間：2018-11-21 17:02
【摘要】：目的 以甲基苯丙胺為代表的新型毒品，其濫用率和依賴性日漸嚴(yán)重。本實(shí)驗(yàn)采用自身給藥模型與認(rèn)知功能藥物干預(yù)的方法，探求青春期不同時(shí)期認(rèn)知功能干預(yù)對(duì)成年期甲基苯丙胺自身給藥以及覓藥行為的影響。 方法 實(shí)驗(yàn)用SD（Sprague-Dawley）大鼠。一半動(dòng)物在青春期20天豐富、匱乏環(huán)境飼養(yǎng)后，進(jìn)行20天的藥物干預(yù)，另一半在青春期30天豐富、匱乏環(huán)境飼養(yǎng)后，進(jìn)行20天的藥物干預(yù)。其中，A和E組：20和30天豐富環(huán)境后，注射MK-801，連續(xù)20天。B和F組，20和30天豐富環(huán)境后，注射生理鹽水，連續(xù)20天，作為A和E組的對(duì)照。C和G組：20和30天匱乏環(huán)境后，注射利培酮，連續(xù)20天。D和H組：20和30天匱乏環(huán)境后，注射生理鹽水，連續(xù)20天，作為C和G組的對(duì)照。藥物干預(yù)結(jié)束，進(jìn)行水迷宮測(cè)試和甲基苯丙胺的自身給藥實(shí)驗(yàn)。甲基苯丙胺自身給藥訓(xùn)練劑量為0.03mg/kg/infusion或0.06mg/kg/infusion，每天4小時(shí)。隨后進(jìn)行FR（固定頻率）、PR（漸變頻率）劑量效應(yīng)測(cè)試，所用劑量為0.015，0.03，，0.06，0.09和0.12mg/kg/infusion。FR測(cè)試每天4小時(shí),PR測(cè)試每天6小時(shí)。戒斷14天后,實(shí)驗(yàn)大鼠進(jìn)行消退及重建測(cè)試。 結(jié)果 1、青春期第20天開始認(rèn)知功能干預(yù)實(shí)驗(yàn)中，水迷宮測(cè)試A組動(dòng)物與控制組B組相比潛伏期顯著增加，C組動(dòng)物與控制組D組相比顯著降低；FR測(cè)試，在甲基苯丙胺劑量為0.015mg/kg/infusion時(shí)，A組動(dòng)物有效鼻觸數(shù)和給藥次數(shù)相比于控制組B組顯著增加，C組相比于控制組D組顯著降低，并且，在甲基苯丙胺劑量為0.03mg/kg/infusion時(shí)，C組動(dòng)物有效鼻觸數(shù)和給藥次數(shù)相比于控制組D組依然顯著性降低，但是，在高劑量甲基苯丙胺0.06，0.09和0.12mg/kg/infusion時(shí)，各組之間有效鼻觸數(shù)和給藥針數(shù)無統(tǒng)計(jì)學(xué)差異；PR測(cè)試，在0.03mg/kg/infusion劑量A組動(dòng)物甲基苯丙胺的斷點(diǎn)數(shù)相比于控制組B組顯著增加，而C組相比于控制組D組顯著降低，其余四個(gè)劑量甲基苯丙胺，實(shí)驗(yàn)動(dòng)物各組之間斷點(diǎn)數(shù)無統(tǒng)計(jì)學(xué)差異；重建測(cè)試中，A組動(dòng)物甲基苯丙胺重建率與控制組B組相比無統(tǒng)計(jì)學(xué)差異，而C組動(dòng)物重建率與控制組D組相比顯著降低。 2、青春期第30天開始認(rèn)知功能干預(yù)實(shí)驗(yàn)中，水迷宮測(cè)試，E組動(dòng)物潛伏期相比于控制組F組無顯著性差異，G組相比于控制組H組顯著降低；FR測(cè)試，五個(gè)甲基苯丙胺劑量下E組動(dòng)物有效鼻觸數(shù)和給藥次數(shù)相比于控制組F組均無顯著性差異，而G組動(dòng)物在0.015mg/kg/infusion和0.03mg/kg/infusion劑量甲基苯丙胺的有效鼻觸數(shù)和給藥次數(shù)相比于控制組H組顯著降低；PR測(cè)試，與FR測(cè)試相似的是，五個(gè)甲基苯丙胺劑量下E組動(dòng)物甲基苯丙胺斷點(diǎn)數(shù)相比于控制組F組無顯著性差異，而G組動(dòng)物在0.03mg/kg/infusion劑量下甲基苯丙胺斷點(diǎn)數(shù)相比于控制組H組顯著降低；重建測(cè)試，E組動(dòng)物甲基苯丙胺重建率與F組相比無顯著性差異，而G組動(dòng)物重建率與控制組H組相比顯著降低。 結(jié)論：匱乏組，無論青春期第20天還是30天開始認(rèn)知功能干預(yù)，均對(duì)大鼠空間記憶、自身給藥行為有影響。豐富組，只有青春期第20天開始藥物干預(yù)才對(duì)大鼠空間記憶、自身給藥行為有影響。而這種差異性主要是由藥物干預(yù)的時(shí)間點(diǎn)不同造成的。認(rèn)知功能增強(qiáng)劑可以逆轉(zhuǎn)匱乏環(huán)境對(duì)大鼠空間記憶、給藥動(dòng)機(jī)、重建率的影響，并且無論青春期第20天還是第30開始干預(yù)，這種影響均顯著；認(rèn)知功能損害劑破壞了豐富環(huán)境對(duì)大鼠空間記憶、給藥動(dòng)機(jī)的保護(hù)作用，并且越早進(jìn)行青春期認(rèn)知功能藥物干預(yù)，這種影響越明顯。
[Abstract]:Purpose New drugs, represented by methamphetamine, with a growing abuse and dependence The effect of cognitive function in the different period of adolescence on the self-administration of methamphetamine and the behavior of drug administration was studied by means of self-administration model and cognitive function drug intervention. influence. Methods SD (Sprague-D, Wley rats. Half of the animals received 20 days of drug intervention after 20 days of puberty, and the other half in the 30-day period of puberty, and the other half took place after 30 days of puberty and in a deficient environment 20-day drug intervention, in which groups A and E: after 20 and 30 days of rich environment, MK-8 was injected 01, 20 days in succession. After 20 and 30 days of environment-rich environment, the saline was injected for 20 consecutive days. Control for Groups A and E. Group C and G: after 20 and 30 days of starvation, injection Capecone, continuous 20 days. Group D and H: after 20 and 30 days of starvation, saline was injected and continued for 20 days. Control for Group C and G. End of drug intervention, water maze test and methylstyrene-acrylic The self-administration of the amine was conducted with a dose of 0. 03mg/ kg/ infl or 0. 06mg/ kg/ infusi. On, 4 hours a day. The FR (fixed frequency), PR (gradient frequency) dose effect test was then performed, and the dose used was 0.015, 0.03, 0.06, 0.09 and 0.12mg/ kg/ infsion. The FR test was 4 hours a day, P R test 6 hours a day. After 14 days of withdrawal, the rats in the experiment line elimination Results 1. The latency of group B in water maze test group was significantly increased in group B compared with control group B, and group C animals were significantly lower than that of control group D. At the time of g/ kg/ infu, the effective nasal and administration times of group A animals were significantly increased compared with control group B, and group C was significantly lower in group C than in control group D, and when the dose of methamphetamine was 0. 03mg/ kg/ infsion, the effective nasal contact number and number of administration of group C animals were compared to control group In group D, there was a significant decrease in group D, but there was no significant difference in the number of effective nasal contact and the number of dosing needles between the groups at high-dose methamphetamine 0. 06, 0. 09 and 0. 12 mg/ kg/ infu. The PR test showed a significant increase in the number of broken points of methamphetamine in the group A of 0. 03mg/ kg/ infu, compared with the control group B group. There was no statistical difference between group C and group B in group C compared with group D, and there was no statistical difference between group B and group B in group A. the construction rate was significantly lower than that of the control group (D). In the first 30 days of puberty, there was no significant difference between the group F and the control group, and the G group was not significantly different from the control group F group. No significant difference was observed in group H compared to control group H; FR test, effective nasal contact number and number of administration of E-group animals in group E compared to control group F group, while group G animals had no significant difference in the number of effective nasal and administration of methamphetamine in 0. 015mg/ kg/ infl and 0. 03mg/ kg/ infu The PR test was similar to the control group H group; similar to the FR test, there was no significant difference in the number of methamphetamine break points in the E group at the five methylamphetamine doses compared to the control group F group, whereas the number of methamphetamine break points in the group G animals at the dose of 0. 03mg/ kg/ infu Compared with the control group, the group H significantly decreased; the reconstruction test showed no significant difference in the recovery rate of the methamphetamine in the E group compared with the group F, and the G group The animal reconstruction rate was significantly lower in comparison with the control group H group. Conclusion: The deficiency group, whether the 20-or 30-day period of puberty, started to cognize the function. Pre-treatment had an effect on the rat's spatial memory and the behavior of its own administration. The effect of the intervention on the rat's spatial memory and its self-administration behavior. The difference is mainly caused by the time point of the drug intervention. The cognitive function enhancer can reverse the effect of the deficient environment on the space memory, the administration motivation and the reconstruction rate of the rat. It is known that the functional damage agent has destroyed the protective effect of the rich environment on the space memory and the administration motivation of the rat, and the earlier it entered
【學(xué)位授予單位】：寧波大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R749.64
本文編號(hào)：2347621
[Abstract]:Purpose New drugs, represented by methamphetamine, with a growing abuse and dependence The effect of cognitive function in the different period of adolescence on the self-administration of methamphetamine and the behavior of drug administration was studied by means of self-administration model and cognitive function drug intervention. influence. Methods SD (Sprague-D, Wley rats. Half of the animals received 20 days of drug intervention after 20 days of puberty, and the other half in the 30-day period of puberty, and the other half took place after 30 days of puberty and in a deficient environment 20-day drug intervention, in which groups A and E: after 20 and 30 days of rich environment, MK-8 was injected 01, 20 days in succession. After 20 and 30 days of environment-rich environment, the saline was injected for 20 consecutive days. Control for Groups A and E. Group C and G: after 20 and 30 days of starvation, injection Capecone, continuous 20 days. Group D and H: after 20 and 30 days of starvation, saline was injected and continued for 20 days. Control for Group C and G. End of drug intervention, water maze test and methylstyrene-acrylic The self-administration of the amine was conducted with a dose of 0. 03mg/ kg/ infl or 0. 06mg/ kg/ infusi. On, 4 hours a day. The FR (fixed frequency), PR (gradient frequency) dose effect test was then performed, and the dose used was 0.015, 0.03, 0.06, 0.09 and 0.12mg/ kg/ infsion. The FR test was 4 hours a day, P R test 6 hours a day. After 14 days of withdrawal, the rats in the experiment line elimination Results 1. The latency of group B in water maze test group was significantly increased in group B compared with control group B, and group C animals were significantly lower than that of control group D. At the time of g/ kg/ infu, the effective nasal and administration times of group A animals were significantly increased compared with control group B, and group C was significantly lower in group C than in control group D, and when the dose of methamphetamine was 0. 03mg/ kg/ infsion, the effective nasal contact number and number of administration of group C animals were compared to control group In group D, there was a significant decrease in group D, but there was no significant difference in the number of effective nasal contact and the number of dosing needles between the groups at high-dose methamphetamine 0. 06, 0. 09 and 0. 12 mg/ kg/ infu. The PR test showed a significant increase in the number of broken points of methamphetamine in the group A of 0. 03mg/ kg/ infu, compared with the control group B group. There was no statistical difference between group C and group B in group C compared with group D, and there was no statistical difference between group B and group B in group A. the construction rate was significantly lower than that of the control group (D). In the first 30 days of puberty, there was no significant difference between the group F and the control group, and the G group was not significantly different from the control group F group. No significant difference was observed in group H compared to control group H; FR test, effective nasal contact number and number of administration of E-group animals in group E compared to control group F group, while group G animals had no significant difference in the number of effective nasal and administration of methamphetamine in 0. 015mg/ kg/ infl and 0. 03mg/ kg/ infu The PR test was similar to the control group H group; similar to the FR test, there was no significant difference in the number of methamphetamine break points in the E group at the five methylamphetamine doses compared to the control group F group, whereas the number of methamphetamine break points in the group G animals at the dose of 0. 03mg/ kg/ infu Compared with the control group, the group H significantly decreased; the reconstruction test showed no significant difference in the recovery rate of the methamphetamine in the E group compared with the group F, and the G group The animal reconstruction rate was significantly lower in comparison with the control group H group. Conclusion: The deficiency group, whether the 20-or 30-day period of puberty, started to cognize the function. Pre-treatment had an effect on the rat's spatial memory and the behavior of its own administration. The effect of the intervention on the rat's spatial memory and its self-administration behavior. The difference is mainly caused by the time point of the drug intervention. The cognitive function enhancer can reverse the effect of the deficient environment on the space memory, the administration motivation and the reconstruction rate of the rat. It is known that the functional damage agent has destroyed the protective effect of the rich environment on the space memory and the administration motivation of the rat, and the earlier it entered
【學(xué)位授予單位】：寧波大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R749.64
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