【摘要】：目的:本研究旨在探索MicroRNA-30e海馬過表達(dá)SD大鼠模型認(rèn)知功能障礙可能的發(fā)病機(jī)制及觀察加味不忘散對該模型大鼠學(xué)習(xí)記憶功能的影響。方法:將40只健康雄性SD大鼠(200g±10g)隨機(jī)分成5組,分別標(biāo)記為空白對照組(簡稱B組)、空載體病毒對照組(簡稱C組)、MicroRNA-30e海馬過表達(dá)模型組(簡稱M組)、加味不忘散低劑量組(簡稱L組)、加味不忘散高劑量組(簡稱H組)。給予M、L、H組大鼠雙側(cè)海馬各注射MicroRNA-30e病毒顆粒1ul;給予C組大鼠雙側(cè)海馬各注射空病毒顆粒1ul;給予B組大鼠雙側(cè)海馬各注射生理鹽水1ul。術(shù)后2周,H、L組分別給予高(0.3g/100g體重)、低(0.15g/100g體重)劑量加味不忘散溶液灌胃,其他三組給以生理鹽水(1ml/100g體重)灌胃。持續(xù)2周后,采用Morris水迷宮實驗評價大鼠學(xué)習(xí)記憶功能。Morris水迷宮實驗完成后將所有大鼠斷頭取腦,分離雙側(cè)海馬組織,制備組織勻漿,利用比色法檢測各樣本ChAT活力的改變。結(jié)果:1.Morris水迷宮實驗結(jié)果:與B組比較,C組各指標(biāo)(每天逃避潛伏期、跨臺次數(shù)及原平臺象限停留時間)變化均無顯著差異(P0.05);與B、C組比較,M組逃避潛伏期在第2-5天顯著延長(p0.05),跨臺次數(shù)顯著減少(p0.05),原平臺象限停留時間顯著縮短(p0.05);與M組比較,L、H組逃避潛伏期在第2-5天顯著縮短(p0.05),跨臺次數(shù)顯著增加(p0.05),原平臺象限停留時間顯著延長(p0.05);與L組比較,H組逃避潛伏期在第2-5天顯著縮短(p0.05),跨臺次數(shù)顯著增加(p0.05),原平臺象限停留時間顯著延長(p0.05)。2.ChAT活力測定結(jié)果:與B組比較,C組海馬ChAT活力變化無顯著差異(P0.05);與B、C組比較,M組海馬ChAT活力顯著降低(p0.05);與M組比較,H、L組海馬ChAT活力均顯著升高(p0.05);與L組比較,H組海馬ChAT活力顯著升高(p0.05)。結(jié)論:1.加味不忘散可顯著改善MicroRNA-30e海馬過表達(dá)SD大鼠學(xué)習(xí)記憶功能,且高、低劑量之間具有濃度依賴性。2.MicroRNA-30e海馬過表達(dá)致SD大鼠認(rèn)知功能障礙的機(jī)制與大鼠海馬ChAT活力降低有關(guān),加味不忘散可提高模型大鼠海馬ChAT活力,且高、低劑量之間具有濃度依賴性。
[Abstract]:Aim: to explore the possible pathogenesis of cognitive dysfunction in MicroRNA-30e hippocampus overexpression SD rat model and to observe the effect of Jiawei Fujisan on learning and memory function in this model. Methods: forty healthy male SD rats (200g 鹵10g) were randomly divided into 5 groups: blank control group (group B), empty carrier virus control group (group C) and MicroRNA-30e hippocampal overexpression model group (M group). The low dose group (L group) and the high dose group (H group) were added. MicroRNA-30e virus particles were injected into both hippocampus of rats in group C, empty virus particles were injected into hippocampus of rats in group C, and normal saline was injected into hippocampus of rats in group B. Two weeks after the operation, the patients in group L were treated with high (0.3g/100g body weight) and low dose (0.15g/100g weight), respectively. The other three groups were treated with normal saline (1ml/100g body weight). After 2 weeks, Morris water maze test was used to evaluate the learning and memory function of rats. After the Morris water maze experiment, all the rats were cut off their brains, the bilateral hippocampal tissues were isolated, and the tissue homogenate was prepared. The changes of ChAT activity in each sample were detected by colorimetric method. Results: the results of 1.Morris water maze test showed that there was no significant difference between group C and group B (P 0.05). Compared with group C, the escape latency of group M was significantly prolonged (p0.05), the number of transmissions was significantly decreased (p0.05), and the residence time of the original platform quadrant was significantly shortened (p0.05). Compared with group M, the escape latency of LHH group was significantly shortened (p0.05), the number of transmissions was significantly increased (p0.05), and the residence time of the original platform quadrant was significantly prolonged (p0.05). Compared with group L, the escape latency of group H was significantly shorter than that of group L (p0.05), and the number of transmissions was significantly increased (p0.05). The residence time of the original platform quadrant was significantly prolonged (p0.05). The results of 2.ChAT activity: compared with group B, there was no significant difference in ChAT activity in hippocampus between group C and group B (P0.05). Compared with group C, the activity of ChAT in hippocampus of group M was significantly lower than that of group B (p0.05), the activity of ChAT in hippocampus of group H was significantly higher than that of group M (p0.05), and the activity of ChAT in hippocampus of group H was significantly higher than that of group L (p0.05). Conclusion: 1. Jiawei Fujisan could significantly improve the learning and memory function of SD rats over-expressed in MicroRNA-30e hippocampus. The mechanism of cognitive dysfunction in SD rats induced by over-expression of 2.MicroRNA-30e hippocampus was related to the decrease of ChAT activity in hippocampus of rats. Jiawei did not forget powder could increase the ChAT activity in hippocampus of model rats. There was a concentration-dependent relationship between low doses.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R749.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 江硯;陳錫林;;絞股藍(lán)總皂苷鎮(zhèn)靜催眠作用及對大鼠腦神經(jīng)遞質(zhì)的影響[J];浙江中醫(yī)雜志;2016年06期

2 陳偉榮;燕毅男;崔紅麗;李新毅;;遠(yuǎn)志總皂苷對阿爾茨海默病模型大鼠海馬腦源性神經(jīng)營養(yǎng)因子及酪氨酸蛋白激酶B表達(dá)的影響[J];中國現(xiàn)代神經(jīng)疾病雜志;2014年05期

3 鄭敏;將裕蕓;周暢;李國營;劉靖;;人參皂苷對衰老大鼠學(xué)習(xí)記憶和腦單胺類神經(jīng)遞質(zhì)的影響[J];宜春學(xué)院學(xué)報;2013年06期

4 劉雪琴;何燕;;淫羊藿苷藥理作用研究進(jìn)展[J];中國中醫(yī)藥科技;2012年04期

5 田素民;馬宇昕;孫靈芝;譚亮;劉靖;李國營;;石菖蒲不同藥效部位改善阿爾茨海默病模型小鼠的認(rèn)知功能[J];中國病理生理雜志;2012年01期

6 馬宇昕;李國營;孫靈芝;譚亮;田素民;;石菖蒲不同提取部位對β淀粉樣蛋白致學(xué)習(xí)記憶障礙模型小鼠的影響[J];神經(jīng)解剖學(xué)雜志;2011年05期

7 賈亮亮;袁丁;王洪武;何毓敏;張長城;;淫羊藿苷藥理作用的研究進(jìn)展[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2010年20期

8 宋景貴;張寧;穆俊林;楊慶南;顧仁駿;;老年性癡呆患者認(rèn)知功能與單胺類神經(jīng)遞質(zhì)的關(guān)系[J];鄭州大學(xué)學(xué)報(醫(yī)學(xué)版);2010年01期

9 金虹;徐國波;黃毅;;石菖蒲抑酶活性的實驗研究[J];時珍國醫(yī)國藥;2009年09期

10 方欣;楊吉平;賴紅;;人參皂甙Rb1對老年性癡呆模型大鼠頂葉皮質(zhì)β-分泌酶及早老蛋白-1表達(dá)的影響[J];解剖學(xué)雜志;2008年06期

相關(guān)會議論文 前1條

1 徐勇;張克讓;;過表達(dá)mir-30e大鼠與認(rèn)知功能障礙[A];中華醫(yī)學(xué)會第十次全國精神醫(yī)學(xué)學(xué)術(shù)會議論文匯編[C];2012年

相關(guān)碩士學(xué)位論文 前6條

1 王海龍;加味不忘散對AD大鼠學(xué)習(xí)記憶及在體海馬長時程增強(qiáng)的影響[D];山西醫(yī)科大學(xué);2015年

2 高琳娜;淫羊藿苷對快速老化小鼠SAMP10學(xué)習(xí)記憶的影響及作用機(jī)制研究[D];河北北方學(xué)院;2012年

3 徐柯樂;遠(yuǎn)志皂苷對Aβ_(1-40)誘導(dǎo)大鼠腦神經(jīng)元凋亡及tau蛋白異常過度磷酸化的保護(hù)作用及其機(jī)制的研究[D];安徽大學(xué);2012年

4 周君;遠(yuǎn)志皂苷對Aβ_（1-40）誘導(dǎo)的AD大鼠大腦神經(jīng)細(xì)胞凋亡及tau蛋白過度磷酸化影響的實驗研究[D];安徽大學(xué);2010年

5 周偉勤;淫羊藿苷對快速老化小鼠SAMP8學(xué)習(xí)記憶的影響及作用機(jī)理研究[D];中國協(xié)和醫(yī)科大學(xué);2009年

6 張媛媛;抑郁大鼠不同腦區(qū)神經(jīng)遞質(zhì)變化的研究[D];中國人民解放軍軍醫(yī)進(jìn)修學(xué)院;2009年

，

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