【摘要】：目的探討Aβ1-42寡聚體側(cè)腦室灌注對(duì)大鼠大腦皮質(zhì)Bcl-2、Caspase-3表達(dá)的影響。方法采用Morris水迷宮法篩選出逃避潛伏期少于60 s的60只雄性大鼠隨機(jī)均分為4組:正常組、PBS對(duì)照組、Aβ1-42纖維組、Aβ1-42寡聚體組。右側(cè)腦室注射Aβ1-42纖維體或Aβ1-42寡聚體復(fù)制阿爾茨海默病(AD)模型;PBS對(duì)照組注射等量的PBS;正常組不作任何處理。模型建立后第4周,采用Morris水迷宮測(cè)試大鼠學(xué)習(xí)記憶能力的變化,用RT-PCR法檢測(cè)大鼠皮質(zhì)Bcl-2、Caspase-3 mRNA的表達(dá),用Western blot法檢測(cè)AD大鼠皮質(zhì)Bcl-2蛋白表達(dá)以及Caspase-3活性。結(jié)果造模后,與正常組和PBS對(duì)照組相比,Aβ1-42纖維組、Aβ1-42寡聚體組逃避潛伏期較造模前均延長(P0.05),Aβ1-42寡聚體組大鼠逃避潛伏期長于Aβ1-42纖維組大鼠的逃避潛伏期;Aβ1-42纖維組及Aβ1-42寡聚體組腦組織中Bcl-2表達(dá)均下調(diào)(P0.05),Caspase-3表達(dá)均上調(diào)(P0.05),以Aβ1-42寡聚體組較為顯著。結(jié)論 Aβ1-42纖維、Aβ1-42寡聚體均可導(dǎo)致大鼠認(rèn)知功能障礙,抑制大鼠皮質(zhì)Bcl-2 mRNA表達(dá),上調(diào)Caspase-3 mRNA表達(dá),活化Caspase-3,其中,Aβ1-42寡聚體對(duì)Bcl-2 mRNA和Caspase-3 mRNA表達(dá)影響較大。
[Abstract]:Objective to investigate the effect of A 尾 1-42 oligomeric lateral ventricular perfusion on the expression of Bcl-2,Caspase-3 in rat cerebral cortex. Methods 60 male rats with escape latency less than 60 s were selected by Morris water maze method. They were randomly divided into 4 groups: normal group, PBS control group, A 尾 1-42 fiber group and A 尾 1-42 oligomer group. The (AD) model of Alzheimer's disease was induced by right ventricle injection of A 尾 1-42 fibrous body or A 尾 1-42 oligomer, while the PBS control group was injected with the same amount of PBS; without any treatment. At the 4th week after the establishment of the model, Morris water maze was used to test the changes of learning and memory ability in rats, and the expression of Bcl-2,Caspase-3 mRNA in cortex of rats was detected by RT-PCR method. The expression of Bcl-2 protein and the activity of Caspase-3 in cortex of AD rats were detected by Western blot method. Results compared with the normal group and PBS control group, the escape latency of A 尾 1-42 oligomer group was significantly longer than that of the control group (P0.05). The escape latency of A 尾 1-42 oligomer group was longer than that of A 尾 1-42 fiber group. In A 尾 1-42 fiber group and A 尾 1-42 oligomer group, the expression of Bcl-2 was down-regulated (P0.05) and the expression of Caspase-3 was up-regulated (P0.05), especially in A 尾 1-42 oligomer group. Conclusion A 尾 1-42 fibers and A 尾 1-42 oligomers can induce cognitive dysfunction, inhibit the expression of Bcl-2 mRNA in rat cortex, up-regulate the expression of Caspase-3 mRNA, and activate Caspase-3,. The expression of Bcl-2 mRNA and Caspase-3 mRNA was significantly affected by A 尾 1-42 oligodeoxynucleotides.
【作者單位】： 桂林醫(yī)學(xué)院解剖學(xué)教研室;河北醫(yī)科大學(xué)神經(jīng)生物研究室;
【基金】：國家自然科學(xué)基金(編號(hào):81260174) 2012年廣西研究生創(chuàng)新項(xiàng)目(編號(hào):YCSW2012108)
【分類號(hào)】：R749.16

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