【摘要】：目的觀(guān)察米諾環(huán)素對(duì)慢性腦低灌注大鼠空間學(xué)習(xí)記憶能力及海馬BACE-1和Aβ表達(dá)的影響,為慢性腦低灌注認(rèn)知功能障礙的治療提供依據(jù)。方法 72只SD大鼠隨機(jī)分為假手術(shù)組、慢性腦低灌注組、米諾環(huán)素治療組。結(jié)扎雙側(cè)頸總動(dòng)脈建立大鼠慢性腦低灌注模型,米諾環(huán)素治療組在慢性腦低灌注模型的基礎(chǔ)上連續(xù)給予50mg/kg/d的米諾環(huán)素灌胃。觀(guān)察時(shí)間點(diǎn)分別為造模后1個(gè)月、2個(gè)月和3個(gè)月。采用Morris水迷宮對(duì)大鼠進(jìn)行定位航行潛伏期和空間探索時(shí)間檢測(cè)后,斷頭取腦,采用免疫組織化學(xué)法檢測(cè)海馬區(qū)腦組織BACE-1和Aβ。結(jié)果在造模后1個(gè)月、2個(gè)月和3個(gè)月時(shí)間點(diǎn),模型組定位航行潛伏期較假手術(shù)組明顯延長(zhǎng)(P0.05,P0.01),空間探索時(shí)間明顯減少(P0.01);治療組潛伏期較模型組明顯縮短(P0.05,P0.01),空間探索時(shí)間明顯增加(P0.05,P0.01)。模型組BACE-1和Aβ的表達(dá)較假手術(shù)組明顯增加(P0.01),治療組BACE-1和Aβ的表達(dá)較模型組顯著降低(P0.05,P0.01)。結(jié)論米諾環(huán)素能改善慢性腦低灌注大鼠空間學(xué)習(xí)記憶能力,其作用機(jī)制可能與其抑制慢性腦低灌注大鼠BACE-1的表達(dá),減少Aβ的產(chǎn)生有關(guān)。
[Abstract]:Objective to observe the effects of minocycline on spatial learning and memory ability and the expression of BACE-1 and A 尾 in hippocampus of rats with chronic cerebral hypoperfusion. Methods 72 SD rats were randomly divided into sham-operation group, chronic cerebral hypoperfusion group and minocycline treatment group. The rat model of chronic cerebral hypoperfusion was established by ligating bilateral common carotid artery. Minocycline group was treated with minocycline (50mg/kg/d) on the basis of chronic cerebral hypoperfusion model. The observed time points were 1 month, 2 months and 3 months after modeling. Morris water maze was used to detect the latency of navigation and the time of space exploration in the rats. After the brain was cut off, the contents of BACE-1 and A 尾 in the hippocampus were detected by immunohistochemical method. Results at the time points of 1 month, 2 months and 3 months after modeling, the latency of positioning navigation in the model group was significantly longer than that in the sham operation group (P0.05, P0.01), and the time of space exploration was significantly reduced (P0.01). Compared with the model group, the latent period of the treatment group was significantly shorter (P0.05 / P0.01), and the time of space exploration was significantly increased (P0.05 / P0.01). The expression of BACE-1 and A 尾 in the model group was significantly higher than that in the sham operation group (P0.01), and the expression of BACE-1 and A 尾 in the treatment group was significantly lower than that in the model group (P0.05, P0.01). Conclusion minocycline can improve the spatial learning and memory ability of rats with chronic cerebral hypoperfusion, and its mechanism may be related to the inhibition of BACE-1 expression and the reduction of A 尾 production in chronic cerebral hypoperfusion rats.
【作者單位】： 遵義醫(yī)學(xué)院第一附屬醫(yī)院神經(jīng)內(nèi)科;遵義醫(yī)學(xué)院第一附屬醫(yī)院病理科;
【基金】：貴州省衛(wèi)生廳科技基金(gzwkj2010-1-050)
【分類(lèi)號(hào)】：R965

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 嚴(yán)潔;劉偉;潘慶敏;;急性腦缺血小鼠血漿β-AP含量動(dòng)態(tài)變化[J];中國(guó)臨床神經(jīng)科學(xué);2006年02期

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本文編號(hào)：2295983