【摘要】：背景 博爾納病病毒（Borna disease virus，BDV）為非節(jié)段單股負(fù)鏈RNA病毒。該病毒無細(xì)胞毒性，且具有高度嗜神經(jīng)細(xì)胞性并且可持續(xù)感染中樞神經(jīng)系統(tǒng)。BDV的自然宿主種類范圍廣泛，據(jù)國內(nèi)外報道BDV可自然或?qū)嶒炇腋腥抉R、兔、鼠、狗等多種溫血類動物。動物感染該病毒后可表現(xiàn)出明顯的精神、行為異常等神經(jīng)精神癥狀。 近年來大量國內(nèi)外的流行病學(xué)研究關(guān)注人與BDV相互關(guān)系，其中許多研究結(jié)果均證實人類可能感染BDV。同時基于血清以及分子流行病學(xué)研究，BDV感染被認(rèn)為與人類抑郁癥等多種精神疾病發(fā)病相關(guān)。 目的 本研究選取重慶地區(qū)抑郁癥患者以及健康人群作為研究對象。采用血清和分子流行病學(xué)研究技術(shù)，探討B(tài)DV在重慶地區(qū)人群中流行病學(xué)規(guī)律，同時比較抑郁癥患者與健康人群中BDV感染分布特點。 方法 1.研究對象納入：采用橫斷面研究方法，經(jīng)過嚴(yán)格的研究對象納入以及排除標(biāo)準(zhǔn)篩選，，本次研究共納入914名研究對象。其中包括抑郁癥入組534人（男性187人，女性347人）和健康對照入組380人（男性160人，女性220人）。 2.血清學(xué)檢測：采用國外報道常用的ELISA方法檢測血漿中BDV循環(huán)免疫復(fù)合物（CICs），每份血漿樣本均重復(fù)測量兩次，取兩次OD值的平均值作為最終結(jié)果。 3.分子生物學(xué)檢測：所有樣本均進(jìn)行分子生物學(xué)檢測。使用全血標(biāo)本提取RNA并逆轉(zhuǎn)錄獲得相應(yīng)的cDNA。其后使用熒光探針法RT-PCR檢測樣本中BDV p24片段。 結(jié)果 1.血清學(xué)檢測結(jié)果：本次檢測共檢出139例BDV循環(huán)免疫復(fù)合物呈不同程度陽性，總體陽性率為15.2%。其中健康對照組檢出42份陽性血漿，陽性率為11.1%；在抑郁癥組中檢出97份陽性血漿，陽性率為18.2%。抑郁組研究對象BDV CICs陽性率明顯高于健康對照組（X~2＝5.093，P=0.024）。 2.分子生物學(xué)檢測結(jié)果：共檢出35份陽性樣本，總體陽性率為3.8%。在抑郁癥組中共檢出27份樣本中含有p24片段，陽性率為5.1%；同時健康對照組中檢出8份，陽性率為2.1%。抑郁組研究對象外周血p24檢出率明顯高于健康對照組（X~2＝5.250，P=0.022）。 結(jié)論 1.本研究首次報道重慶地區(qū)抑郁癥患者以及健康人群中人感染BDV的流行病學(xué)研究數(shù)據(jù)。 2.本研究血清學(xué)和分子生物學(xué)結(jié)果均提示在抑郁癥患者中BDV感染率顯著高于健康人群，與國外相關(guān)研究結(jié)果吻合。提示BDV感染可能與精神疾病相關(guān)。
[Abstract]:Background Borna disease virus (Borna disease virus,BDV) is a non-segmental single-stranded negative-stranded RNA virus. The virus is non-cytotoxic, highly neurophilic and sustainably infects the central nervous system. There are a wide range of natural hosts for BDV. It is reported at home and abroad that BDV can infect horses, rabbits, mice and mice naturally or in laboratory. Dogs and other warm-blooded animals. Animals infected with the virus can show obvious mental, behavioral disorders and other neuropsychiatric symptoms. In recent years, a large number of epidemiological studies at home and abroad have focused on the relationship between human beings and BDV, many of which have confirmed that humans may be infected with BDV.. At the same time, based on serological and molecular epidemiological studies, BDV infection is thought to be associated with various mental disorders such as depression in humans. Objective to study depression patients and healthy people in Chongqing. The epidemiological characteristics of BDV in Chongqing population were studied by using serum and molecular epidemiology techniques, and the distribution characteristics of BDV infection in depression patients and healthy people were compared at the same time. Method 1. Inclusion of study subjects: 914 subjects were included in this study through strict selection of inclusion and exclusion criteria using cross-sectional research method. These included 534 depression patients (187 men and 347 women) and 380 healthy controls (160 men and 220 women). Serological detection: the BDV circulating immune complex (CICs),) in plasma was detected by ELISA method reported abroad. The average value of OD in each plasma sample was measured twice and the average value of OD was taken as the final result. 3. Molecular biology detection: all samples were tested by molecular biology. RNA was extracted from whole blood samples and cDNA. was obtained by reverse transcription. The BDV p24 fragment was detected by fluorescence probe RT-PCR. Result 1. Serological results: a total of 139 cases of BDV circulating immune complex were positive in different degrees, and the overall positive rate was 15.22%. 42 positive plasma samples were detected in the healthy control group, the positive rate was 11.1, and 97 positive plasma samples were found in the depression group, the positive rate was 18.2%. The positive rate of BDV CICs in depression group was significantly higher than that in healthy control group (X2 + 5.093). Results of molecular biology: 35 positive samples were detected, the total positive rate was 3.8%. The positive rate of p24 fragment was 5.1 in 27 samples of depression group and 8 in healthy control group, the positive rate was 2.1%. The detection rate of p24 in peripheral blood in depression group was significantly higher than that in healthy control group (X2 + 5. 250 P + 0. 022). Conclusion 1. This study reported for the first time the epidemiological data of BDV infection among depression patients and healthy people in Chongqing. 2. The results of serology and molecular biology showed that the infection rate of BDV in depression patients was significantly higher than that in healthy people. The results suggest that BDV infection may be associated with mental illness.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R749.4

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