【摘要】：目的探索漢族群體中甲基苯丙胺依賴相關(guān)的差異表達(dá)基因。方法采集14例漢族甲基苯丙胺依賴者以及8例年齡、性別相匹配正常人的外周全血,利用mRNA表達(dá)譜芯片對(duì)其進(jìn)行基因表達(dá)譜分析,并對(duì)差異表達(dá)基因進(jìn)行功能分類分析。結(jié)果在27 958條轉(zhuǎn)錄本中,實(shí)驗(yàn)組和對(duì)照組差異表達(dá)倍數(shù)在2倍或2倍以上的轉(zhuǎn)錄本有3 560條,其中上調(diào)表達(dá)基因1 510個(gè),下調(diào)表達(dá)基因2 576個(gè)。對(duì)這些差異基因分析發(fā)現(xiàn)甲基苯丙胺對(duì)細(xì)胞組分、分子功能、生物學(xué)過程的負(fù)調(diào)節(jié)有重要影響,并與轉(zhuǎn)錄調(diào)控、核苷酸代謝和修飾、蛋白質(zhì)修飾、細(xì)胞凋亡、應(yīng)激調(diào)節(jié)、自身免疫反應(yīng)等相關(guān)。結(jié)論甲基苯丙胺成癮的發(fā)生涉及多基因表達(dá)的改變,芯片技術(shù)可以有效地篩選出甲基苯丙胺依賴者與正常對(duì)照的差異表達(dá)基因,對(duì)進(jìn)一步探索甲基苯丙胺成癮機(jī)制、有效的干預(yù)或逆轉(zhuǎn)毒品成癮具有重要意義。
[Abstract]:Objective to explore the differentially expressed genes related to methamphetamine dependence in Han population. Methods Peripheral blood samples were collected from 14 patients with methamphetamine dependence in Han nationality and 8 healthy subjects of age and sex. The gene expression profiles were analyzed by mRNA microarray and the functional classification of differentially expressed genes was performed. Results among the 27 958 transcripts, there were 3 560 transcripts with 2 or more times of differential expression, including 1 510 up-regulated genes and 2 576 down-regulated genes. It was found that methamphetamine had an important effect on the negative regulation of cell composition, molecular function and biological process, and related to transcription regulation, nucleotide metabolism and modification, protein modification, cell apoptosis, stress regulation. Autoimmune response and so on. Conclusion the occurrence of methamphetamine addiction involves the change of polygene expression. Microarray technique can effectively screen differentially expressed genes between methamphetamine addicts and normal controls, and further explore the mechanism of methamphetamine addiction. Effective intervention or reversal of drug addiction is of great significance.
【作者單位】： 南京森林警察學(xué)院國(guó)家林業(yè)局森林公安司法鑒定中心;南京森林警察學(xué)院刑事科學(xué)技術(shù)系;
【基金】：南京森林警察學(xué)院國(guó)家自然科學(xué)基金預(yù)研項(xiàng)目(LGY201502)
【分類號(hào)】：R749.6

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