發(fā)布時(shí)間：2018-10-17 12:06

【摘要】：目的 調(diào)查天津市老年人認(rèn)知功能障礙(cognitive impairment, CI)患病率,并分析其影響因素；探究載脂蛋白E基因多態(tài)性和老年認(rèn)知功能的相關(guān)性；觀察藍(lán)莓花色苷對輕度認(rèn)知功能障礙(mild cognitive impairment, MCI)患者認(rèn)知功能及炎性因子分泌的影響,為老年癡呆癥早期營養(yǎng)干預(yù)措施的制訂提供科學(xué)依據(jù)。 方法 1.采用橫斷面研究,調(diào)查505名天津市老年人CI患病率,并分析其影響因素。 2.采用聚合酶鏈?zhǔn)椒磻?yīng)-限制性片段長度多態(tài)性(polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP)方法隨機(jī)檢測160名天津市老年人的ApoE基因型,采用簡易精神狀態(tài)量表(mini-mental state examination, MMSE)、基本認(rèn)知能力(Basic cognitive aptitude test,BCAT)軟件測試?yán)夏耆说恼J(rèn)知功能,并分析ApoEε4基因?qū)夏耆苏J(rèn)知功能及CI患病率的影響。 3.以篩查出的MCI患者46人為研究對象,分為干預(yù)組和對照組,每組各23人,分別給予藍(lán)莓花色苷沖劑和飲用水,連續(xù)干預(yù)12周。研究對象均知情同意。采用BCAT測試軟件、視覺反應(yīng)測試及數(shù)字記憶廣度測試檢測認(rèn)知功能,采用酶聯(lián)免疫法(ELISA)檢測血清白細(xì)胞介素-6(interleukin-6,IL-6)、腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)濃度。 結(jié)果 1.505名調(diào)查對象的CI患病率為25.90%,主要影響因素為年齡、鍛煉時(shí)間和收入水平,其中年齡是CI患病的危險(xiǎn)因素,鍛煉時(shí)間、收入水平是CI患病的保護(hù)因素；天津市老年人認(rèn)知功能的主要影響因素為文化程度、鍛煉時(shí)間、飲水量、收入水平、年齡和日常生活活動(dòng)能力,文化程度、鍛煉時(shí)間、飲水量、收入水平與MMSE分?jǐn)?shù)呈正相關(guān),而年齡、ADL分?jǐn)?shù)與MMSE分?jǐn)?shù)呈負(fù)相關(guān)。 2.160名調(diào)查對象中攜帶ApoEε4基因者35人,主要影響老年人的整體認(rèn)知功能、知覺速度和工作記憶；攜帶ApoEs4基因者M(jìn)MSE分?jǐn)?shù)、BCAT分測試知覺速度和工作記憶得分及BCAT測試總分顯著低于非攜帶ApoEε4基因者,差異有統(tǒng)計(jì)學(xué)意義(p0.05),且CI患者的ApoEε4基因攜帶率明顯高于非CI患者,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。 3.與干預(yù)前比較,藍(lán)莓花色苷干預(yù)組老人BCAT測試總分、知覺速度、心算效率、空間表象效率及工作記憶分測試得分均明顯提高,差異有統(tǒng)計(jì)學(xué)意義(p0.05),同時(shí)數(shù)字記憶廣度測試得分明顯提高,差異有統(tǒng)計(jì)學(xué)意義(p0.05),血清IL-6、TNF-α濃度無明顯變化；與對照組比較,干預(yù)組老人空間表象效率、工作記憶、記憶再認(rèn)分測試得分及BCAT測試總分明顯提高,差異有統(tǒng)計(jì)學(xué)意義(p0.05),視覺反應(yīng)測試中隨機(jī)奇偶反應(yīng)時(shí)明顯縮短,差異有統(tǒng)計(jì)學(xué)意義(p0.05),血清IL-6、TNF-α濃度無明顯變化。 結(jié)論 文化程度、鍛煉時(shí)間、飲水量、收入水平、年齡和日常生活活動(dòng)能力以及ApoEs4基因是影響老年認(rèn)知功能的主要因素；藍(lán)莓花色苷干預(yù)可明顯改善MCI患者的認(rèn)知功能。
[Abstract]:Objective to investigate the prevalence of (cognitive impairment, CI) in the elderly with cognitive dysfunction in Tianjin and analyze its influencing factors, and to explore the correlation between apolipoprotein E gene polymorphism and cognitive function in the elderly. To observe the effect of blueberry anthocyanin on cognitive function and inflammatory factor secretion in patients with mild cognitive impairment (mild cognitive impairment, MCI) and to provide scientific basis for early nutritional intervention in Alzheimer's disease. Method 1. A cross-sectional study was conducted to investigate the prevalence of CI in 505 elderly people in Tianjin and to analyze its influencing factors. Polymerase chain reaction-restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP) was used to detect ApoE genotypes in 160 elderly people in Tianjin. The simple mental state scale (mini-mental state examination, MMSE), basic cognitive ability (Basic cognitive aptitude test,BCAT) was used to test the cognitive function of the elderly, and the effect of ApoE 蔚 4 gene on the cognitive function and the prevalence of CI was analyzed. 3. Forty-six patients with MCI were divided into intervention group (n = 23) and control group (n = 23). They were treated with blueberry anthocyanin granules and drinking water for 12 weeks. All the subjects were informed and agreed. BCAT test software, visual response test and digital memory span test were used to detect cognitive function. Serum interleukin-6 (interleukin-6,IL-6), tumor necrosis factor- 偽 (TNF- 偽) and serum levels of interleukin-6 (interleukin-6,IL-6) and tumor necrosis factor- 偽 (TNF- 偽) were detected by enzyme linked immunosorbent assay (ELISA). Results the prevalence rate of CI in 1.505 subjects was 25.90. The main influencing factors were age, exercise time and income level, among which age was the risk factor of CI disease, exercise time and income level were the protective factors of CI. The main factors influencing the cognitive function of the elderly in Tianjin were education level, exercise time, drinking water, income level, age and ability of daily life activities, education level, exercise time, drinking water, income level and MMSE score. Age, ADL score and MMSE score were negatively correlated. 35 subjects with ApoE 蔚 4 gene carried ApoE 蔚 4 gene in 2.160 subjects, which mainly affected the overall cognitive function, perceptual speed and working memory of the elderly. The MMSE score, BCAT score and total BCAT score of patients with ApoEs4 gene were significantly lower than those without ApoE 蔚 4 gene (p0.05), and the ApoE 蔚 4 gene carrying rate of CI patients was significantly higher than that of non-CI patients. The difference was statistically significant (p0.05). Compared with those before intervention, the total score of BCAT, the rate of perception, the efficiency of mental arithmetic, the efficiency of spatial representation and the score of working memory of the elderly in the blueberry anthocyanin intervention group were significantly improved. The difference was statistically significant (p0. 05), and the scores of digital memory span test were significantly improved (p0. 05), the serum IL-6,TNF- 偽 concentration was not changed significantly, compared with the control group, the elderly in the intervention group had the spatial representation efficiency and working memory. The score of memory recognition test and the total score of BCAT test were significantly increased (p0.05), the time of random odd-even reaction was shortened significantly in visual response test (p0.05), the difference was statistically significant (p0.05), the concentration of serum IL-6,TNF- 偽 did not change significantly. Conclusion Education level, exercise time, drinking water, income level, age, ability of daily life and ApoEs4 gene are the main factors affecting cognitive function of the elderly. Blueberry anthocyanin intervention can significantly improve the cognitive function of MCI patients.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R749.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張映琦,周華東,李敬誠,張猛,王延江,高長越,鄧娟,何洪波;日常生活活動(dòng)能力等因素對老年人認(rèn)知功能的影響[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2003年22期

2 李志武;黃悅勤;柳玉芝;;中國65歲以上老年人認(rèn)知功能及影響因素調(diào)查[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2007年16期

3 李國才,招偉賢;載脂蛋白E基因多態(tài)性與認(rèn)知損害[J];國外醫(yī)學(xué).麻醉學(xué)與復(fù)蘇分冊;2005年04期

4 陳煜森;許志恩;趙斌;山縣英久;三木哲郎;;ApoE基因多態(tài)性與阿爾茨海默病的相關(guān)性研究[J];神經(jīng)疾病與精神衛(wèi)生;2006年02期

5 封丹;;第六次人口普查顯示:中國人口老齡化趨勢加快[J];科技智囊;2011年06期

6 麥以成;羅有年;顧鴻;幺憲偉;付燕;鄭洪波;胡文生;黎艷;吳仲揚(yáng);林靜靜;;載脂蛋白E基因多態(tài)性與Alzheimer病的關(guān)聯(lián)研究[J];臨床精神醫(yī)學(xué)雜志;2007年06期

7 高錦云;李建民;陳長香;趙雅寧;;ApoE基因及相關(guān)因素與缺血性腦卒中認(rèn)知障礙關(guān)系的研究進(jìn)展[J];華北煤炭醫(yī)學(xué)院學(xué)報(bào);2011年03期

8 朱詠新,周維智,林琳,劉曉紅,陸青,朱晴暉;載脂蛋白E基因多態(tài)性與老年人認(rèn)知功能相關(guān)性研究[J];神經(jīng)疾病與精神衛(wèi)生;2001年03期

9 楊紅澎;蔣與剛;崔玉山;龐偉;盧豪;盧士軍;程義勇;;藍(lán)莓等10種果蔬提取物體外抗氧化活性的比較[J];食品研究與開發(fā);2010年11期

10 任艷峰,曲成毅,苗茂華;太原市4社區(qū)老年人認(rèn)知功能的影響因素[J];山西醫(yī)科大學(xué)學(xué)報(bào);2005年01期

，

本文編號：2276603