發(fā)布時(shí)間：2018-09-04 17:56

【摘要】：目的探索非典型抗精神病藥利培酮對大鼠腦區(qū)中腦源性神經(jīng)生長因子(brain-derived neurotrophic factor,BDNF)及其受體酪氨酸激酶受體B(tyrosine kinase receptor,TrkB)、P75神經(jīng)營養(yǎng)因子受體(P75neurotrophin receptor,P75NTR)的調(diào)控作用。方法將大鼠隨機(jī)分為利培酮處理組(n=8)和對照組(n=8),利培酮處理組采用0.25mg/kg利培酮腹腔內(nèi)注射,對照組采用等量安慰劑腹腔內(nèi)注射,連續(xù)處理14d后處死大鼠。取大鼠左右前額葉皮質(zhì)、左右顳葉皮質(zhì)及海馬,提取RNA并進(jìn)行BDNF、TrκB和P75 NTR mRNA的實(shí)時(shí)熒光定量PCR分析。結(jié)果利培酮處理組大鼠的左右前額葉皮質(zhì)、左右顳葉皮質(zhì)及海馬中BDNF及TrkB mRNA的表達(dá)水平較對照組增高(P0.05),而利培酮處理組P75 NTR mRNA的表達(dá)與對照組間差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論利培酮能夠上調(diào)大鼠左右前額葉皮質(zhì)、左右顳葉皮質(zhì)及海馬BDNF-TrkB信號通路,而不能改變上述腦區(qū)中P75 NTR mRNA的表達(dá)水平。
[Abstract]:Objective to investigate the regulatory effect of risperidone, an atypical antipsychotic drug, on brain-derived nerve growth factor (brain-derived neurotrophic factor,BDNF) and its receptor tyrosine kinase receptor (B (tyrosine kinase receptor,TrkB) P75 neurotrophic factor receptor (P75neurotrophin receptor,P75NTR) in rat brain. Methods Rats were randomly divided into two groups: risperidone treated group and control group. The rats in risperidone treated group were injected intraperitoneally with 0.25mg/kg risperidone and those in control group were injected intraperitoneally with the same amount of placebo. The rats were sacrificed after 14 days of continuous treatment. RNA was extracted from left and right prefrontal cortex, left and right temporal lobe cortex and hippocampus, and real-time quantitative PCR analysis of BDNF,Tr 魏 B and P75 NTR mRNA was performed. Results the expression of BDNF and TrkB mRNA in prefrontal cortex, left and right temporal cortex and hippocampus of rats treated with risperidone was higher than that of control group (P0.05), while the expression of P75 NTR mRNA in risperidone treated group was not significantly different from that in control group (P0.05). Conclusion Risperidone can up-regulate the BDNF-TrkB signal pathway in the left and right prefrontal cortex, left and right temporal cortex and hippocampus of rats, but can not change the expression of P75 NTR mRNA in these brain regions.
【作者單位】： 四川大學(xué)華西醫(yī)院精神醫(yī)學(xué)研究室心理衛(wèi)生中心;
【基金】：國家自然科學(xué)基金(No.81071089,No.81201042) 2013年度高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金(新教師類)(No.20130181120032)資助
【分類號】：R749.3

【共引文獻(xiàn)】

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