【摘要】：目的 :觀察甲基苯丙胺(methamphetamine,Meth)暴露后神經(jīng)病理性蛋白β-淀粉樣前體蛋白(amyloid precursor protein,APP)及p-tau表達(dá)的改變。方法 :將30只C57BL/6J雄性小鼠隨機(jī)分為對(duì)照組、生理鹽水組,Meth組,Meth組腹腔注射給予10 mg/kg的劑量,1 d 2次,連續(xù)7 d。利用高爾基銀染法觀察Meth作用后大腦神經(jīng)元數(shù)量及棘突的改變;采用Western blot法檢測(cè)Meth暴露后神經(jīng)病理性蛋白APP,p-tau表達(dá)的改變。結(jié)果:高爾基銀染結(jié)果顯示,Meth組與生理鹽水組及對(duì)照組比較,神經(jīng)元數(shù)量及棘突顯著減少。通過Western blot發(fā)現(xiàn)Meth能劑量依賴及時(shí)間依賴性上調(diào)病理性蛋白β-APP及p-tau的表達(dá),具有顯著性差異(P0.05)。此外,通過預(yù)孵L型鈣通道的抑制劑硝苯地平,發(fā)現(xiàn)Meth引起的APP及p-tau上調(diào)顯著被抑制。結(jié)論:Meth暴露可引起阿爾茲海默樣病變,因而該研究可為Meth引起認(rèn)知能力下降提供部分解釋。
[Abstract]:Aim: to observe the expression of 尾 -amyloid precursor protein (amyloid precursor protein,APP) and p-tau after exposure to methamphetamine (methamphetamine,Meth). Methods: thirty male C57BL/6J mice were randomly divided into two groups: the control group (n = 30) and the control group (n = 30). The rats in the control group were given intraperitoneal injection of 10 mg/kg for 1 d for 7 days. The changes of the number of neurons and spinous process in the brain after Meth were observed by Golgi silver staining, and the expression of neuropathic protein APP,p-tau after Meth exposure was detected by Western blot method. Results: the results of Golgi silver staining showed that the number of neurons and spinous process in Meth group were significantly lower than those in normal saline group and control group. Western blot showed that Meth could up-regulate the expression of 尾 -APP and p-tau in dose-dependent and time-dependent manner (P0.05). In addition, the up-regulation of APP and p-tau induced by Meth was significantly inhibited by nifedipine, an inhibitor of L-type calcium channel. ConclusionMeth exposure may cause Alzheimer like lesions, so this study may provide a partial explanation for cognitive impairment induced by Meth.
【作者單位】： 南京醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;南京醫(yī)科大學(xué)第一附屬醫(yī)院急診醫(yī)學(xué)中心;
【基金】：國家自然科學(xué)基金(81202230,81673213) 江蘇省自然科學(xué)基金(BK20151557) 江蘇省科技廳重點(diǎn)病種規(guī)范化診療研究(BL2014088)
【分類號(hào)】：R749.64

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