豐富環(huán)境對擬老年性癡呆小鼠學習記憶和神經(jīng)元突觸可塑性影響研究
發(fā)布時間：2018-07-28 14:57
【摘要】：目的：本實驗研究豐富環(huán)境對擬老年性癡呆(Alzheimer disease,AD)小鼠學習記憶能力的影響,并探討其可能的作用機制,為豐富環(huán)境干預(yù)AD臨床推廣應(yīng)用提供理論依據(jù)。 方法：健康昆明種小鼠40只隨機分為標準環(huán)境對照組、標準環(huán)境模型組、豐富環(huán)境模型組和豐富環(huán)境對照組,每組10只。采用側(cè)腦室注射凝聚態(tài)Aβ1-40建立擬AD小鼠模型,Morris水迷宮實驗測試學習記憶能力；透射電鏡觀察海馬CA1區(qū)突觸數(shù)密度(Nv)、突觸面密度(Sv)及平均面積(S)的變化；用免疫組化和圖象分析方法檢測海馬CA1區(qū)突觸素表達。 結(jié)果：1. Morris水迷宮實驗結(jié)果：標準環(huán)境模型組與標準環(huán)境對照組比較平均逃避潛伏期延長(P0.05),跳臺次數(shù)減少(P0.05)。豐富環(huán)境模型組與標準環(huán)境模型組比較平均逃避潛伏期時間縮短(P0.05),跳臺次數(shù)增多(P0.05)。 2.突觸形態(tài)結(jié)構(gòu)可塑性檢測顯示：標準環(huán)境對照組較標準環(huán)境模型組突觸數(shù)密度和面密度較大(P0.01),連接帶平均面積也較小(P0.01)。豐富環(huán)境模型組較標準環(huán)境模型組突觸數(shù)密度和面密度明顯增大(P0.01),連接帶平均面積也相對較小(P0.01)。 3.突觸素免疫組化檢測結(jié)果：標準環(huán)境對照組和豐富環(huán)境對照組突觸素表達最多,豐富環(huán)境模型組次之、標準環(huán)境模型組表達最少。通過檢測光密度值定量分析顯示：標準環(huán)境模型組與標準環(huán)境對照組比較突觸素的平均光密度值明顯降低(P0.01)；與標準環(huán)境模型組比較,豐富環(huán)境模型組的光密度均值明顯增高(P0.01)。 結(jié)論：1.豐富環(huán)境能改善AD小鼠學習記憶能力。 2.豐富環(huán)境可減輕AD小鼠突觸的損傷,并促進新突觸與突觸連接的形成,促進突觸的重構(gòu)。豐富環(huán)境能改善AD小鼠學習記憶能力可能與突觸可塑性有關(guān)。 3.豐富環(huán)境可使AD小鼠海馬區(qū)突觸素的表達增多,促使突觸的重構(gòu),從而改善AD小鼠學習記憶能力。
[Abstract]:Objective: to study the effect of rich environment on learning and memory ability of Alzheimer disease (AD) mice and explore its possible mechanism. Methods: forty healthy Kunming mice were randomly divided into three groups: the standard environment control group, the standard environment model group, the enriched environment model group and the rich environment control group, with 10 mice in each group. The rat model of AD was established by injecting condensed matter A 尾 1-40 into the lateral ventricle. The ability of learning and memory was tested by Morris water maze test, and the changes of synaptic surface density (Sv) and average area (S) (S) of synaptic number density (Nv),) in hippocampal CA1 region were observed by transmission electron microscope (TEM). Synaptophysin expression in hippocampal CA1 was detected by immunohistochemistry and image analysis. The result is 1: 1. Morris water maze test results: standard environmental model group compared with the standard environment control group average escape latency prolonged (P0.05), the number of platform jumping decreased (P0.05). Compared with the standard environmental model group, the average escape latency time of the rich environment model group was shorter (P0.05), and the frequency of platform jumping increased (P0.05). The synaptic morphological and structural plasticity test showed that the number density and surface density of synapses in the standard environment control group were higher than those in the standard environmental model group (P0.01), and the average area of the junction band was also smaller (P0.01). Compared with the standard environmental model group, the synaptic number density and surface density of the rich environment model group increased significantly (P0.01), and the average area of the junction band was also relatively small (P0.01). The expression of synaptophysin was the most in the standard environment control group and the rich environment control group, followed by the rich environment model group and the standard environment model group. Quantitative analysis of optical density showed that the average optical density of synaptophysin in the standard environment model group was significantly lower than that in the standard environment control group (P0.01), and that in the enriched environment model group was significantly higher than that in the standard environment model group (P0.01). Conclusion 1. Rich environment can improve the learning and memory ability of AD mice. 2. Enriched environment can reduce synaptic damage, promote the formation of new synapses and synaptic connections, and promote synaptic remodeling in AD mice. Rich environment can improve learning and memory ability of AD mice, which may be related to synaptic plasticity. 3. 3. Rich environment can increase the expression of synaptophysin in hippocampal area of AD mice, promote synaptic remodeling, and improve the learning and memory ability of AD mice.
【學位授予單位】：遵義醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R749.16
本文編號：2150607
[Abstract]:Objective: to study the effect of rich environment on learning and memory ability of Alzheimer disease (AD) mice and explore its possible mechanism. Methods: forty healthy Kunming mice were randomly divided into three groups: the standard environment control group, the standard environment model group, the enriched environment model group and the rich environment control group, with 10 mice in each group. The rat model of AD was established by injecting condensed matter A 尾 1-40 into the lateral ventricle. The ability of learning and memory was tested by Morris water maze test, and the changes of synaptic surface density (Sv) and average area (S) (S) of synaptic number density (Nv),) in hippocampal CA1 region were observed by transmission electron microscope (TEM). Synaptophysin expression in hippocampal CA1 was detected by immunohistochemistry and image analysis. The result is 1: 1. Morris water maze test results: standard environmental model group compared with the standard environment control group average escape latency prolonged (P0.05), the number of platform jumping decreased (P0.05). Compared with the standard environmental model group, the average escape latency time of the rich environment model group was shorter (P0.05), and the frequency of platform jumping increased (P0.05). The synaptic morphological and structural plasticity test showed that the number density and surface density of synapses in the standard environment control group were higher than those in the standard environmental model group (P0.01), and the average area of the junction band was also smaller (P0.01). Compared with the standard environmental model group, the synaptic number density and surface density of the rich environment model group increased significantly (P0.01), and the average area of the junction band was also relatively small (P0.01). The expression of synaptophysin was the most in the standard environment control group and the rich environment control group, followed by the rich environment model group and the standard environment model group. Quantitative analysis of optical density showed that the average optical density of synaptophysin in the standard environment model group was significantly lower than that in the standard environment control group (P0.01), and that in the enriched environment model group was significantly higher than that in the standard environment model group (P0.01). Conclusion 1. Rich environment can improve the learning and memory ability of AD mice. 2. Enriched environment can reduce synaptic damage, promote the formation of new synapses and synaptic connections, and promote synaptic remodeling in AD mice. Rich environment can improve learning and memory ability of AD mice, which may be related to synaptic plasticity. 3. 3. Rich environment can increase the expression of synaptophysin in hippocampal area of AD mice, promote synaptic remodeling, and improve the learning and memory ability of AD mice.
【學位授予單位】：遵義醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R749.16
【參考文獻】
相關(guān)期刊論文 前6條
1 吳冰潔;顧平;崔冬生;耿媛;王銘維;;豐富環(huán)境對快速老化小鼠SAMP8學習記憶能力的影響[J];第二軍醫(yī)大學學報;2007年09期
2 常一丁;;不同中藥組方對癡呆大鼠行為學及突觸素水平影響的研究[J];中國熱帶醫(yī)學;2007年01期
3 龍大宏,姚志彬,何蘊韶,陳以慈;神經(jīng)生長因子對老年鼠穹窿海馬傘損傷后齒狀回膽堿能突觸重構(gòu)的作用[J];神經(jīng)解剖學雜志;1997年01期
4 鄭昆幼,林菁;臥床老年患者壓瘡發(fā)生危險因素量化評估[J];中國臨床康復(fù);2003年15期
5 洪樂鵬;龍大宏;冷水龍;;癡呆模型鼠腦海馬突觸素改變及其與學習記憶的關(guān)系(英文)[J];中國臨床康復(fù);2005年36期
6 王威;李崧;董會萍;呂申;唐一源;;不同劑量D-半乳糖致昆明小鼠腦老化模型實驗的研究[J];中國老年學雜志;2009年03期
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