【摘要】：脂多糖(lipopolysaccharide,LPS)免疫激活模型是研究抑郁癥細胞因子假說的重要動物模型,目前國際上常用外周單次LPS注射誘發(fā)抑郁樣行為,但該模型中抑郁樣行為持續(xù)僅有數小時。為建立誘發(fā)較長時程抑郁樣行為的免疫激活動物模型,本研究嘗試側腦室注射LPS激活大鼠中樞免疫炎性反應,考察單次以及重復中樞LPS注射誘發(fā)抑郁樣行為的效果,以及中樞炎性免疫誘發(fā)行為改變的時程。結果顯示:單次中樞LPS注射后24 h只能誘發(fā)曠場自發(fā)活動和探索行為下降等部分抑郁樣行為,未能誘導糖水偏好下降和懸尾不動時間增加;3次重復注射則在末次LPS注射后24 h表現出顯著的糖水偏好下降,自發(fā)活動和探索行為減少,懸尾不動時間增加等行為。且自發(fā)活動、探索行為減少和懸尾不動時間增加能夠延續(xù)至末次LPS注射后72 h。這些結果表明腦室重復LPS注射可誘發(fā)較長時程的抑郁樣行為,這種新的中樞炎性免疫激活誘發(fā)的抑郁癥模型,為研究抑郁癥炎性免疫機制提供了更為有效的動物模型,有助于深入探討行為和免疫功能間的復雜關系。
[Abstract]:Lipopolysaccharide (LPS) immunoreactive model is an important animal model to study the cytokine hypothesis of depression. At present, depression like behavior is induced by single injection of LPS in the world, but the depressive behavior in this model lasts only a few hours. In order to establish an immune-activated animal model of long-lasting depressive behavior, we tried to induce the central immune inflammatory response in rats by intraventricular injection of LPS, and to investigate the effect of single and repeated central LPS injection on depressive behavior in rats. And the time course of behavior change induced by central inflammatory immunity. The results showed that 24 h after a single injection of LPS, only some depressive behaviors such as spontaneous open field activity and decreased exploratory behavior were induced. The decrease of sugar water preference and the increase of tail suspension immobility time after 24 hours of LPS injection showed significant decrease of sugar water preference, decrease of spontaneous activity and exploration behavior, and increase of tail suspension immobility time. Spontaneous activity, decreased exploratory behavior and increased tail immobility could last until 72 hours after the last LPS injection. These results suggest that repeated injection of LPS can induce long-term depressive behavior. This new model of depression induced by central inflammatory immune activation provides a more effective animal model for studying the inflammatory immune mechanism of depression. It is helpful to explore the complex relationship between behavior and immune function.
【作者單位】： 中國科學院心理健康重點實驗室 中國科學院心理研究所;中國科學院大學;
【基金】：國家自然科學基金項目(30770718,31170987) 中國科學院知識創(chuàng)新項目(KSCX2-EW-J-8)資助
【分類號】：R749.4

【參考文獻】

相關期刊論文 前2條

1 潘玉芹;王東林;林文娟;;脂多糖免疫激活導致抑郁性行為的動物研究:劑量和時程效應[J];心理學報;2007年06期

2 池少鵬;戚智;姬廣聚;鄺雪瑩;林文娟;;脂多糖激活所致大鼠抑郁樣行為及對海馬神經細胞鉀電流變化的影響[J];心理學報;2012年04期

【共引文獻】

相關期刊論文 前10條

1 鄭蕾;王藝明;;帕羅西汀治療抑郁癥后血清神經營養(yǎng)因子3的變化[J];貴陽醫(yī)學院學報;2013年04期

2 楊靜;烏力吉圖;張力青;;氣動聲波潔治術結合鹽酸米諾環(huán)素凝膠對根分叉Ⅱ度病變的療效分析[J];第三軍醫(yī)大學學報;2013年20期

3 Urmila M Aswar;Padmaja P Kalshetti;Suhas M Shelke;Sharad H Bhosale;Subhash L Bodhankar;;Effect of newly synthesized 1,2,4-triazino[5,6-b]indole-3-thione derivatives on olfactory bulbectomy induced depression in rats[J];Asian Pacific Journal of Tropical Biomedicine;2012年12期

4 Parastoo Karimian;Gholamreza Kavoosi;Zahra Amirghofran;;Anti-oxidative and anti-inflammatory effects of Tagetes minuta essential oil in activated macrophages[J];Asian Pacific Journal of Tropical Biomedicine;2014年03期

5 左燦;李海英;卿之駒;陳遠林;;急性時相反應蛋白與精神分裂癥后抑郁的關系[J];廣東醫(yī)學;2013年21期

6 黃丹;賴天文;陳敏;伍俊;招軒娜;梁敏;吳斌;;哮喘患者抑郁心理對血清總IgE、FEV1%預計值和嗜酸性粒細胞絕對值的影響[J];廣東醫(yī)學院學報;2014年05期

7 黎麗;李婭;黎曼儂;張倩;王U，

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