發(fā)布時(shí)間：2018-06-23 13:10

  本文選題：創(chuàng)傷后應(yīng)激障礙 + 腫瘤壞死因子-α　； 參考：《華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2016年04期

【摘要】：目的通過(guò)檢測(cè)創(chuàng)傷后應(yīng)激障礙(post-traumatic stress disorder,PTSD)大鼠海馬中吲哚胺2,3-雙加氧酶(indoleamine 2,3-dioxygenase,IDO)含量變化與運(yùn)用IDO抑制劑治療對(duì)神經(jīng)元細(xì)胞的保護(hù)機(jī)制,探討PTSD發(fā)病原因及機(jī)制。方法將60只健康雄性Wistar大鼠隨機(jī)分為:對(duì)照組、PTSD組、PTSD+IDO抑制劑治療組,運(yùn)用ELISA試劑盒檢測(cè)腫瘤壞死因子-α(tumor necrosis factor,TNF-α)、白介素-6(interleukin-6,IL-6)含量變化,通過(guò)RT-PCR、Western blot檢測(cè)IDO的表達(dá)情況。通過(guò)TUNEL染色法檢測(cè)海馬神經(jīng)元凋亡率,并對(duì)大鼠進(jìn)行行為學(xué)評(píng)估。結(jié)果檢測(cè)結(jié)果顯示對(duì)照組、PTSD組與IDO抑制劑治療組TNF-α分別為[(1.26±0.12)vs.(8.58±0.67)vs.(3.69±0.41)pg/mL]、IL-6分別為[(2.28±0.19)vs.(15.72±1.42)vs.(7.45±0.58)pg/mL]、IDOmRNA分別為[(0.152 7±0.014 7)vs.(0.827 8±0.079 6)vs.(0.223 6±0.038 7)]、IDO蛋白分別為[(0.061 2±0.008 6)vs.(1.232 9±0.114 8)vs.(0.423 5±0.041 1)]、神經(jīng)元的凋亡率為[(5.46±1.87)%vs.(81.47±6.86)%vs.(42.54±3.98)%](均P0.05),行為學(xué)表現(xiàn)明顯改善。結(jié)論 PTSD大鼠海馬區(qū)域TNF-α、IL-6、IDO表達(dá)顯著提高,IDO抑制劑治療能降低其含量,細(xì)胞因子、IDO在PTSD發(fā)病機(jī)制中起重要作用,運(yùn)用IDO抑制劑能改善PTSD大鼠海馬區(qū)域神經(jīng)元損傷。
[Abstract]:Objective to investigate the changes of indoleamine 3-dioxygenase (indoleamine _ 2) _ 3-dioxygenase (IDO) content in hippocampus of rats with post-traumatic stress disorder (post-traumatic stress) and the protective mechanism of IDO inhibitor therapy on neuronal cells, and to explore the pathogenesis and mechanism of the pathogenesis of indoleamine 3-dioxygenase (IDO) in the hippocampus of rats with post-traumatic stress disorder (PTSD). Methods Sixty healthy male Wistar rats were randomly divided into two groups: the control group was treated with PTSD-IDO inhibitor. The levels of tumor necrosis factor- 偽 (tumor necrosis factor-TNF- 偽 and interleukin-6 (IL-6) were detected by Elisa kit, and the expression of Ido was detected by RT-PCR- Western blot. The apoptotic rate of hippocampal neurons was detected by Tunel staining, and the behavior of rats was evaluated. 緇撴灉媯，

本文編號(hào)：2057219