本文選題：VDR + 基因多態(tài)性　； 參考：《中南大學(xué)》2013年碩士論文

【摘要】：一、目的 探討VDR基因多態(tài)性與中國精神分裂癥的相關(guān)性；比較首發(fā)精神分裂癥患者服用利培酮前和4周后胰島素與血脂四項水平的差異,并研究胰島素和血脂水平變化與VDR基因多態(tài)性的相關(guān)性。 二、方法 1.基因分型 采用PCR-LDR技術(shù)對VDR基因的四個位點Fok-I(RS2228570)、 Cdx-2(RS11568820)、Bsm-I(RS1544410)、Apa-I(RS7975232)進行分型。 2.VDR基因多態(tài)性與中國精神分裂癥相關(guān)性研究 收集精神分裂癥患者222例作為病例組,150例健康體檢者作為健康對照組,分別對病例組和對照組的VDR基因進行分型和統(tǒng)計比較。 3.VDR基因多態(tài)性與利培酮誘導(dǎo)胰島素和血脂變化的相關(guān)性研究 選擇首次發(fā)病、未服用抗精神病藥的住院精神分裂癥患者29例,單用利培酮治療,分別檢測服藥前和服藥4周后的空腹胰島素和血脂四項水平,并對這29例病例VDR基因進行分型。 所有數(shù)據(jù)統(tǒng)計均采用SPSS20.0軟件進行。 三、結(jié)果 1.VDR基因多態(tài)性與精神分裂癥的相關(guān)性 病例組和對照組的VDR基因型和基因頻率的分布無顯著差異；相同性別的病例組和對照組基因分布無顯著差異；各基因型的平均發(fā)病年齡無顯著差異；男性和女性平均發(fā)病年齡分別為(21.99±6.05)歲和(24.42±7.97)歲,差異無統(tǒng)計學(xué)意義(P=0.121)。 2.VDR基因多態(tài)性與利培酮誘導(dǎo)胰島素和血脂變化的相關(guān)性 服用利培酮4周后,胰島素和甘油三酯水平顯著升高(p0.05)。Fok-Ⅰ基因的不同基因型,胰島素水平存在顯著性差異(0周P=0.019,4周P=0.002),利培酮治療后胰島素變化也具有顯著差異(P=0.005),CC基因型患者胰島素升高最顯著；不同Apa-I基因型,服藥4周后TG水平具有顯著差異(P=0.030),利培酮治療后TC(P=0.028)和TG變化(P=0.041)存在顯著差異,AA基因型攜帶者TC和TG變化較其他基因型更大。 四、結(jié)論 1.VDR基因的四個位點Fok-I、Cdx-2、Bsm-I、Apa-I與中國精神分裂癥無顯著相關(guān)性。 2.服用利培酮4周后胰島素和TG水平顯著升高。 3.Fok-I與利培酮誘導(dǎo)的胰島素升高相關(guān),CC基因型胰島素升高最大；Apa-I基因的AA基因型攜帶者更容易產(chǎn)生血脂異常的不良反應(yīng)。
[Abstract]:First, the purpose
To investigate the correlation between VDR gene polymorphism and Chinese schizophrenia, compared the four levels of insulin and blood lipid levels before and 4 weeks after risperidone in patients with schizophrenia, and the correlation between the changes of insulin and blood lipids and the polymorphism of VDR gene.
Two, method
1. genotyping
PCR-LDR technology was used to classify the four loci of VDR gene Fok-I (RS2228570), Cdx-2 (RS11568820), Bsm-I (RS1544410) and Apa-I (RS7975232).
Association between 2.VDR gene polymorphism and schizophrenia in China
222 cases of schizophrenic patients were collected as case group and 150 healthy subjects were used as healthy control group. The VDR gene of case group and control group were divided and compared.
Association of 3.VDR gene polymorphisms with risperidone induced changes in insulin and blood lipids
29 hospitalized schizophrenic patients who had not taken antipsychotic drugs for the first time were selected and treated with risperidone alone. Four levels of fasting insulin and blood lipid were detected before and 4 weeks after taking medicine, and the VDR gene was typed in these 29 cases.
All data statistics are carried out by SPSS20.0 software.
Three, the result
Association of 1.VDR gene polymorphism with schizophrenia
There was no significant difference in the distribution of VDR genotypes and gene frequencies between the case group and the control group, and there was no significant difference in the distribution of gene distribution in the same sex group and the control group; the average age of onset of the genotypes had no significant difference, and the average age of the male and female was (21.99 + 6.05) and (24.42 + 7.97) years, respectively (P =0.121).
Association of 2.VDR gene polymorphisms with risperidone induced changes in insulin and blood lipids
After 4 weeks of risperidone, the levels of insulin and triglyceride increased significantly (P0.05) in different genotypes of.Fok- I gene, the insulin level was significantly different (0 weeks P=0.019,4 weeks P=0.002), and the changes of insulin after risperidone were also significantly different (P=0.005), and the most significant increase in insulin in the CC genotype patients; the different Apa-I genotypes, After 4 weeks, the level of TG was significantly different (P=0.030). There was a significant difference in TC (P=0.028) and TG changes (P=0.041) after risperidone treatment. The TC and TG changes of the AA genotype carriers were larger than those of the other genotypes.
Four. Conclusion
There are no significant correlations between the four loci of 1.VDR gene Fok-I, Cdx-2, Bsm-I, Apa-I and schizophrenia in China.
2. risperidone increased the level of insulin and TG after 4 weeks of risperidone treatment.
3.Fok-I is associated with risperidone induced insulin rise, and CC genotype insulin increases most; the AA genotype carriers of the Apa-I gene are more likely to produce adverse reactions to dyslipidemia.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R749.3

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