本文選題：阿爾茨海默病 + 載脂蛋白E　； 參考：《中國老年學(xué)雜志》2017年07期

【摘要】：目的探討截?cái)嘈?trauncated)載脂蛋白(Apo)E對(duì)β-淀粉樣蛋白代謝的影響。方法對(duì)N2a細(xì)胞進(jìn)行培養(yǎng),利用pEGFP-ApoE4或pEGFP-T-ApoE4質(zhì)粒進(jìn)行轉(zhuǎn)染后制備條件培養(yǎng)基,利用熒光法檢測(cè)全長ApoE4和truncated-ApoE4與Aβ42的結(jié)合能力,并對(duì)與Aβ42結(jié)合復(fù)合物對(duì)SDS的穩(wěn)定性進(jìn)行檢測(cè),利用熒光顯微鏡對(duì)truncated-ApoE4和全長ApoE4對(duì)影響N2a細(xì)胞結(jié)合和攝取Aβ42情況進(jìn)行觀察。結(jié)果當(dāng)Aβ42濃度≤100 nmol/L時(shí),truncated-ApoE4組熒光強(qiáng)度均低于全長ApoE4組;進(jìn)行含SDS聚丙烯凝膠電泳時(shí),兩組均在6 k D的位置處出現(xiàn)條帶,而在43 k D處的條帶明顯變窄,truncated-ApoE4組大約是原來的30%,ApoE4組大約為原來的55%,兩組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05);truncated-ApoE4組N2a細(xì)胞結(jié)合和攝取Aβ42數(shù)量均少于全長ApoE4組(P0.05)。結(jié)論 truncated-ApoE4與Aβ42結(jié)合能力減弱,形成的復(fù)合物穩(wěn)定性降低,可以顯著影響N2a細(xì)胞對(duì)Aβ42的結(jié)合和攝取作用,抑制truncated-ApoE4產(chǎn)生為AD預(yù)防和治療提供新的研究方向。
[Abstract]:Objective to investigate the effect of apolipoprotein ApoE on 尾 -amyloid protein metabolism. Methods N2a cells were cultured and transfected with pEGFP-ApoE4 or pEGFP-T-ApoE4 plasmid. The binding ability of full-length ApoE4 and truncated-ApoE4 to A 尾 42 was detected by fluorescence method. The effects of truncated-ApoE4 and full-length ApoE4 on the binding and uptake of A 尾 42 in N2a cells were observed by fluorescence microscopy. Results when A 尾 42 concentration 鈮，

本文編號(hào)：2033564