本文選題：抑郁模型大鼠 + 茶氨酸��； 參考：《中南大學》2013年博士論文

【摘要】：目的： 觀察茶葉提取物茶氨酸及氟西汀對抑郁模型大鼠行為學及前額葉皮質(zhì)(PFC)、伏隔核(NAC)、紋狀體(ST)、杏仁核(AM)和海馬(HIP)等邊緣系統(tǒng)—皮層—紋狀體—蒼白球—丘腦(LCSPT)回路相關(guān)腦區(qū)單胺遞質(zhì)及谷氨酸遞質(zhì)的影響,探討茶氨酸可能的抗抑郁機制。 研究方法： 1.動物模型：選取雄性SD大鼠66只,隨機分為模型組(17只)、氟西汀組(17只)、茶氨酸組(17只)、對照組(15只),前三組采用連續(xù)21天慢性輕度不可預(yù)見性應(yīng)激(CUMS)結(jié)合孤養(yǎng)的方法建立慢性抑郁大鼠模型,茶氨酸組和氟西汀組分別給予2mg/kg/Qd茶氨酸和1.54mg/kg/Qd氟西汀灌胃21天,模型組和對照組給予同體積溶劑灌胃21天。 2.行為學指標：實驗第1天,第22天,各組取9只大鼠,記錄實驗過程中各組大鼠的體重、曠場試驗的活動總路程、糖水偏愛試驗的糖水偏愛值、和強迫游泳試驗的不動時間,研究茶氨酸及氟西汀對上述指標的影響。 3.神經(jīng)遞質(zhì)指標：實驗第22天,各組取8只大鼠(對照組6只),處死大鼠,取出PFC、NAC、ST、AM和HIP等腦組織,用庫侖陣列電化學高效液相色譜法測定各組抑郁模型大鼠上述腦區(qū)單胺遞質(zhì)(NE、5-HT、HIP)的濃度及其代謝產(chǎn)物和谷氨酸(GLU)遞質(zhì)及谷氨酰胺(GLN)的濃度。 結(jié)果： 1.與對照組相比,模型組大鼠反應(yīng)遲鈍,體重增加減慢,在規(guī)定時間內(nèi)自主水平活動減少,糖水消耗量、糖水偏愛百分比降低,強迫游泳試驗的不動時間增長(P0.05)。與模型組相比,茶氨酸組和氟西汀組大鼠體重增加速度正常、自主運動能力增強,糖水消耗量、糖水偏愛百分比顯著增加,強迫游泳試驗的不動時間減少(P0.05)。 2.與對照組相比,模型組大鼠PFC、NAC、ST (NE陰性)、AM (NE、5-HT陰性)、HIP等腦區(qū)單胺神經(jīng)遞質(zhì)NE、DA和5-HT及部分代謝產(chǎn)物的濃度明顯降低,GLU(GLN)濃度明顯升高(P0.05)。 3.與模型組相比,氟西汀組PFC、NAC、ST (NE陰性)、AM(NE\5HT陰性)、HIP(NE陰性)等腦區(qū)單胺神經(jīng)遞質(zhì)濃度及部分代謝產(chǎn)物的濃度顯著升高,GLU (GLN)的濃度顯著降低(P0.05)。 與模型組相比,茶氨酸組PFC、NAC、ST (NE陰性)、AM(5HT陰性)、HIP等腦區(qū)單胺神經(jīng)遞質(zhì)濃度及部分代謝產(chǎn)物的濃度顯著升高,GLU (GLN)的濃度顯著降低(P0.05) 結(jié)論： 1.抑郁模型大鼠各項行為學指標異常,前額葉、伏隔核、海馬、紋狀體(NE陰性)、杏仁核(NE、5-HT陰性)等腦區(qū)NE、5-HT、 DA濃度下降,GLU濃度上升,表明抑郁模型大鼠存在抑郁行為和不同腦區(qū)神經(jīng)遞質(zhì)改變,說明模型建立成功。 2.茶氨酸和氟西汀均可以有效改善抑郁模型大鼠的抑郁行為,并提高PFC、NAC、HIP、AM、ST等腦區(qū)單胺神經(jīng)遞質(zhì)NE、5-HT、 DA及部分代謝產(chǎn)物的濃度,降低GLU及其前體GLN的濃度,說明茶氨酸抗抑郁作用與可能機制和氟西汀類似。
[Abstract]:Objective: Effects of tea extract theanine and fluoxetine on behavior and LCSPTs in prefrontal cortex of rats with depression were observed, such as PFCN, NACN, STT, AMAM and HIPP), such as cortex-striatum, globus pallidus and thalamus (LCSPTs). Effects of monoamine and glutamate transmitters in Guannao region, To explore the possible antidepressant mechanism of theanine. Research methods: 1. Animal models: 66 male SD rats were selected, The rats were randomly divided into three groups: model group (n = 17), fluoxetine group (n = 17), theanine group (n = 17) and control group (n = 15). Theanine group and fluoxetine group were given 2mg/kg/Qd theanine and 1.54mg/kg/Qd fluoxetine for 21 days, and the model group and control group were given the same volume solvent for 21 days. 2. Behavioral indicators: on day 1 and day 22, 9 rats were taken from each group. The body weight, the total distance of open field test, the sugar water preference value of sugar water preference test, and the immobility time of forced swimming test were recorded. To study the effects of theanine and fluoxetine on these indexes. 3. Neurotransmitter index: on the 22nd day of the experiment, 8 rats in each group (6 rats in the control group) were killed, and the brain tissues such as HIP and HIP were taken out. Coulomb array electrochemical high performance liquid chromatography (HPLC) was used to determine the concentration of monoamine transmitter NE5-HT HIPs, its metabolites, glutamate GLU transmitters and glutamine (GLN) in the brain of rats with depression. Results: 1. Compared with the control group, the rats in the model group were retarded in reaction, weight gain slowed down, the activity of their own level decreased, the consumption of sugar water, the percentage of preference for sugar water decreased, and the immobility time of forced swimming test increased by 0.05%. Compared with the model group, theanine group and fluoxetine group showed normal weight gain rate, increased spontaneous exercise ability, increased sugar water consumption, percentage of sugar water preference, and reduced the immobility time of forced swimming test (P 0.05). 2. Compared with the control group, the concentration of monoamine neurotransmitters NEDA, 5-HT and some metabolites in the brain regions of the model group were significantly lower than those in the control group, and the concentration of GLU GLN in the brain regions of the model group was significantly higher than that of the control group, and the concentration of the monoamine neurotransmitters NEDA, 5-HT and some metabolites in the brain regions of the model group was significantly higher than that of the control group. 3. Compared with the model group, the concentration of monoamine neurotransmitters and some metabolites in fluoxetine group was significantly higher than that in the model group. The concentration of monoamine neurotransmitters and some metabolites in fluoxetine group was significantly higher than that in fluoxetine group. Compared with the model group, the concentration of monoamine neurotransmitters and some metabolites in the brain regions of the PFCnAC-NE-negative and AM5HT negative brain regions were significantly higher than that of the model group, and the concentration of GLU (GLN) decreased significantly (P 0.05). Conclusion: 1. The behavioral parameters of depression model rats were abnormal, the brain regions of prefrontal lobe, nucleus accumbens, hippocampus, striatum were negative for NE, and the amygdala was negative for NE-5HT), but the concentration of DA decreased and GLU increased. The results showed that there were depressive behaviors and neurotransmitter changes in different brain regions in the depression model rats, which indicated that the model was established successfully. 2. Theanine and fluoxetine can effectively improve the depressive behavior of rats with depression, and increase the concentration of monoamine neurotransmitter NE5-HT, DA and some metabolites in the brain, such as PFCnAC-HIPAMST, and decrease the concentration of GLU and its precursor GLN. This suggests that theanine has a similar antidepressant effect to the possible mechanism and fluoxetine.
【學位授予單位】：中南大學
【學位級別】：博士
【學位授予年份】：2013
【分類號】：R749.4;R-332

【參考文獻】

相關(guān)期刊論文 前10條

1 馬妮;陳林慶;藺興遙;陸立偉;周鵬;;慢性應(yīng)激抑郁癥大鼠模型的復(fù)制與評價[J];甘肅中醫(yī)學院學報;2010年06期

2 龔雨順;李勤;黃建安;劉仲華;;茶氨酸制備方法研究進展[J];湖南農(nóng)業(yè)大學學報(自然科學版);2008年02期

3 曹燕翔;姚志劍;謝世平;杜經(jīng)綸;王麗;劉海燕;;抑郁癥患者識別動態(tài)面部表情的執(zhí)行功能研究[J];臨床精神醫(yī)學雜志;2007年04期

4 林雪玲,程朝輝,黃才歡,李炎;茶氨酸對小鼠學習記憶能力的影響[J];食品科學;2004年05期

5 湯球;劉志學;崔淑芳;蔡麗萍;;大鼠抑郁癥模型的建立與評價[J];實驗動物科學;2011年01期

6 夏軍,葉慧,周義成,張景峰,夏黎明,王承緣;慢性應(yīng)激大鼠抑郁模型的建立及其有效性的探討[J];華中科技大學學報(醫(yī)學版);2005年04期

7 姚輝,陶領(lǐng)綱,張曉斌,沙維偉,侯鋼,張志s，

本文編號：1976380