發(fā)布時間：2018-06-01 16:38

  本文選題：CYP27B1 + 基因多態(tài)性��； 參考：《中南大學》2013年碩士論文

【摘要】：目的：本研究主要目的是探討CYP27B1基因多態(tài)性與精神分裂癥發(fā)病的關(guān)聯(lián),以及與利培酮治療后引起的代謝指標變化是否有關(guān)。方法：入組222例精神分裂癥住院患者及150例健康志愿者,采用聚合酶連接檢測反應技術(shù)(polymerase chain reaction-ligase detection reaction, PCR-LDR)檢測rs10877012和rs4646536基因型。采用德國西門子拜耳ADVIA Centaur自動化學發(fā)光免疫分析儀直接測定胰島素(insulin, Ins);采用柏林格曼姆日立7170自動生化分析儀檢測血脂四項(TC、TG、HDLC. LDLC)。數(shù)據(jù)采用SPSS16.0軟件分析。結(jié)果：采用χ2檢驗分析得到所有基因型符合Hardy-Weinberg平衡,rs10877012和rs4646536基因多態(tài)性與精神分裂癥發(fā)病無統(tǒng)計學意義(P0.05)。利培酮治療4周后胰島素與TG較0周有統(tǒng)計學意義(P0.05), TC、HDLC、LDLC無統(tǒng)計學意義,P0.05。采用方差分析得到rs10877012基因型與0周(P=0.012)及服藥4周胰島素變化值(p=0.019)有統(tǒng)計學意義,rs4646536基因型與0周(P=0.013)及4周胰島素變化值(P=0.022)有統(tǒng)計學意義。結(jié)論：CYP27B1rs10877012和rs4646536兩個基因多態(tài)性和精神分裂癥的發(fā)病無統(tǒng)計學意義,說明CYP27B1基因多態(tài)性與中國中南地區(qū)漢族精神分裂癥發(fā)病無關(guān)。精神分裂癥患者用利培酮單藥治療4周后胰島素、TG水平較0周水平顯著升高。rs10877012基因型與利培酮治療后胰島素變化有關(guān),攜帶T等位基因的患者胰島素更易升高。rs4646536基因型與0周及服用利培酮4周胰島素變化有關(guān),攜帶C等位基因的患者服藥治療后胰島素更易升高,說明CYP27B1基因多態(tài)性可能與利培酮治療后胰島素耐受有關(guān)。圖10幅,表17個,參考文獻44篇。
[Abstract]:Objective: to investigate the association between CYP27B1 gene polymorphism and schizophrenia and whether it is related to metabolic changes after risperidone treatment. Methods: rs10877012 and rs4646536 genotypes were detected by polymerase chain reaction-ligase detection reaction (PCR-LDR) in 222 inpatients with schizophrenia and 150 healthy volunteers. Insulin and insulin were directly determined by Siemens Bayer ADVIA Centaur automatic chemiluminescence immunoassay (ADVIA Centaur), and the serum lipids were determined by Berlin Gumman Hitachi 7170 automatic biochemical analyzer. LDLC. The data was analyzed by SPSS16.0 software. Results: 蠂 2 analysis showed that all genotypes were consistent with Hardy-Weinberg balance rs10877012 and rs4646536 gene polymorphisms, and there was no significant difference between the polymorphism of rs10877012 and the incidence of schizophrenia (P 0.05). After 4 weeks of risperidone treatment, there was significant difference in insulin and TG between 0 week and 0 week (P 0.05), but there was no significant difference in LDLC between TCX HDLC and TG (P 0.05). The analysis of variance showed that there were significant differences between the genotype of rs10877012 and 0 week (P 0.012) and the change value of insulin at 4 weeks (P 0.019). There was statistical significance between genotype rs4646536 and 0 week (P < 0.013) and the change value of insulin at 4 weeks (P = 0.022). Conclusion there is no significant difference between the two gene polymorphisms of CYP27B1rs10877012 and rs4646536 and the incidence of schizophrenia, indicating that the polymorphism of CYP27B1 gene is not related to the onset of schizophrenia in the Han nationality in the central and southern regions of China. Insulin TG level in schizophrenia patients was significantly higher than that at 0 weeks after risperidone treatment for 4 weeks. Genotype rs10877012 was associated with insulin changes after risperidone treatment. Insulin of patients with T allele was more likely to increase. Rs4646536 genotype was associated with insulin changes at 0 weeks and risperidone at 4 weeks, and insulin was more likely to increase in patients with C allele after treatment with risperidone. The results suggest that CYP27B1 gene polymorphism may be associated with insulin resistance after risperidone therapy. Ten figures, 17 tables, 44 references.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R749.3

【參考文獻】

相關(guān)期刊論文 前2條

1 黃楠;陸崢;;精神分裂癥急性期的藥物治療進展[J];世界臨床藥物;2010年04期

2 姚志劍,張志s，

本文編號：1964914