本文選題：丁香酚 + 血管性癡呆��； 參考：《安徽醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的 本實(shí)驗(yàn)采用改良的2VO法建立慢性腦缺血VD模型，觀察丁香酚吸嗅后VD模型大鼠學(xué)習(xí)障礙記憶改善的情況；并使用免疫組化法檢測(cè)丁香酚吸嗅后大鼠海馬組織中Glu能神經(jīng)元的變化情況，，初步探討其作用機(jī)制。 方法 1.建立VD大鼠模型：用改良的2VO法造模，即先結(jié)扎右側(cè)頸總動(dòng)脈，1周后用同樣的方法結(jié)扎左側(cè)頸總動(dòng)脈。 2.丁香酚對(duì)VD模型大鼠學(xué)習(xí)障礙記憶改善及機(jī)制：成年雄性SD大鼠45只隨機(jī)分成3組： （1）VD模型組（n=18）：用改良的2VO方法造模，①在造模完成后7d、30d、60d時(shí)，用Morris水迷宮測(cè)定大鼠的空間學(xué)習(xí)記憶能力，記錄定位航行實(shí)驗(yàn)中大鼠到達(dá)安全平臺(tái)的時(shí)間（即為逃避潛伏期）和空間探索實(shí)驗(yàn)中的大鼠穿越原平臺(tái)的次數(shù)（即為穿臺(tái)次數(shù)）為實(shí)驗(yàn)指標(biāo)，時(shí)間都以120s為限。②在行為學(xué)實(shí)驗(yàn)結(jié)束后，大鼠斷頭取腦，制作石蠟切片，HE染色觀察大鼠海馬區(qū)病理形態(tài)學(xué)變化，應(yīng)用免疫組化法測(cè)定大鼠海馬區(qū)的Glu能神經(jīng)元的變化情況。 （2）實(shí)驗(yàn)組（n=17）:手術(shù)的操作步驟同模型組，在造模完成后3d后予以1%濃度的丁香酚吸嗅，上、下午各1次，每次30min，同時(shí)行與模型組相同的操作步驟。 （3）空白組（n=10）：不做任何手術(shù)處理，余實(shí)驗(yàn)步驟同模型組。 結(jié)果 1.Morris水迷宮實(shí)驗(yàn)中，術(shù)后7d時(shí)，模型組、實(shí)驗(yàn)組、空白組大鼠的逃避潛伏期、穿臺(tái)次數(shù)之間差異均無(wú)統(tǒng)計(jì)學(xué)意義（P0.05）。 2.術(shù)后30d時(shí)，實(shí)驗(yàn)組大鼠逃避潛伏期顯著短于模型組，差異有統(tǒng)計(jì)學(xué)意義（P0.05）；穿臺(tái)次數(shù)顯著多于模型組，差異有統(tǒng)計(jì)學(xué)意義（P0.05）。模型組大鼠逃避潛伏期顯著長(zhǎng)于空白組,差異有統(tǒng)計(jì)學(xué)意義（P0.05）；穿臺(tái)次數(shù)顯著少于空白組.差異有統(tǒng)計(jì)學(xué)意義（P0.05）。 3.術(shù)后60d時(shí)，實(shí)驗(yàn)組大鼠的逃避潛伏期顯著短于模型組（P0.01），穿臺(tái)次數(shù)顯著多于模型組（P0.01）。模型組大鼠的逃避潛伏期顯著長(zhǎng)于空白組,差異有顯著統(tǒng)計(jì)學(xué)意義（P0.01）；穿臺(tái)次數(shù)顯著少于空白組,差異有顯著統(tǒng)計(jì)學(xué)意義（P0.01）。 4.模型組大鼠在術(shù)后7d、30d和60d時(shí)，逃避潛伏期、穿臺(tái)次數(shù)前后相比，差異有統(tǒng)計(jì)學(xué)意義（P0.05）；實(shí)驗(yàn)組大鼠在術(shù)后7d、30d和60d時(shí)，逃避潛伏期、穿臺(tái)次數(shù)前后相比，差異有統(tǒng)計(jì)學(xué)意義（P0.05）。 5.免疫組化結(jié)果中，模型組大鼠海馬區(qū)Glu陽(yáng)性神經(jīng)元的數(shù)量較空白組大鼠顯著減少（P0.01），實(shí)驗(yàn)組大鼠腦組織海馬區(qū)Glu陽(yáng)性神經(jīng)元的數(shù)量較模型組大鼠顯著增加（P0.01）。 結(jié)論 1.改良的2VO法建立大鼠VD模型，對(duì)大鼠腦組織損傷較小，且會(huì)出現(xiàn)明顯的學(xué)習(xí)記憶障礙，符合VD臨床的特點(diǎn)。 2.在腦缺血后的60d內(nèi)，隨著缺血時(shí)間的延長(zhǎng)，大鼠學(xué)習(xí)記憶障礙會(huì)越嚴(yán)重。 3.丁香酚吸嗅后，VD模型大鼠的學(xué)習(xí)記憶障礙可明顯改善。 4.丁香酚改善VD模型大鼠的學(xué)習(xí)記憶能力與抑制海馬區(qū)Glu能神經(jīng)元的下降有關(guān)。
[Abstract]:Purpose The VD model of chronic cerebral ischemia was established by modified 2VO method to observe the improvement of learning disability and memory in VD rats after eugenol sniffing. The changes of Glu neurons in rat hippocampal tissue after eugenol sniffing were detected by immunohistochemical method. Method 1. The model of VD rats was established by modified 2VO method. The right common carotid artery was ligated for 1 week and then left common carotid artery was ligated in the same way. 2. Effects of eugenol on learning impairment and memory in VD rats: Forty-five adult male SD rats were randomly divided into 3 groups: In the VD model group, the modified 2VO method was used to model the rats. The spatial learning and memory abilities of the rats were measured by Morris water maze at 30 days and 60 days after the completion of the model. The time to reach the safe platform (i.e. escape latency) and the number of times that the rats in the space exploration experiment crossed the original platform (i.e. the number of platform piercing) were recorded as the experimental indicators. The time limit was 120s. 2. After the behavioral experiment was finished, the rat head was cut off and his staining was made to observe the pathomorphological changes of the hippocampus, and the changes of Glu neurons in the hippocampus were measured by immunohistochemical method. (2) in the experimental group, the procedure of operation was the same as that in the model group, and 1% eugenol was given to the model group 3 days after the completion of the model, once in the afternoon, 30 minutes each time, at the same time as in the model group. Blank group: no surgical treatment, the rest of the experimental steps are the same as the model group. Result In the 1.Morris water maze test, there was no significant difference in the escape latency and the number of perforations between the model group, the experimental group and the blank group on the 7th day after operation (P 0.05). 2. At 30 days after operation, the escape latency of the experimental group was significantly shorter than that of the model group, and the difference was statistically significant (P 0.05), and the number of perforations was significantly higher than that of the model group (P 0.05). The escape latency of the model group was significantly longer than that of the blank group, the difference was statistically significant (P 0.05), and the number of platform piercing was significantly lower than that of the blank group. The difference was statistically significant (P 0.05). 3. At 60 days after operation, the escape latency of the experimental group was significantly shorter than that of the model group (P 0.01), and the number of perforations was significantly higher than that of the model group (P 0.01). The escape latency of the model group was significantly longer than that of the blank group, the difference was statistically significant (P 0.01), and the frequency of platform penetration was significantly lower than that of the blank group (P 0.01). 4. In the model group, there was a significant difference in the escape latency and the times of platform penetration between 30 and 60 days after the operation, while in the experimental group, there was a significant difference in the escape latency between 30 and 60 days after operation, and the difference between the two groups before and after the operation was statistically significant (P 0.05). 5. In the immunohistochemical results, the number of Glu positive neurons in the hippocampus of the model group was significantly lower than that of the control group, and the number of Glu positive neurons in the hippocampus of the experimental group was significantly higher than that of the model group. Conclusion 1. The modified 2VO method was used to establish VD model in rats. The damage to brain tissue was small, and obvious learning and memory disorder appeared, which was in line with the clinical characteristics of VD. 2. Within 60 days after cerebral ischemia, the learning and memory impairment would become more serious with the prolongation of ischemic time. 3. The learning and memory impairment of VD rats after eugenol sniffing can be improved significantly. 4. The effect of eugenol on learning and memory in VD rats was related to the inhibition of the decrease of Glu neurons in hippocampus.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R285.5;R749.1

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