發(fā)布時(shí)間：2018-05-07 10:10

  本文選題：創(chuàng)傷后應(yīng)激障礙 + 端粒長(zhǎng)度��； 參考：《石河子大學(xué)》2016年碩士論文

【摘要】：第一部分經(jīng)SPS創(chuàng)傷后PTSD樣大鼠端粒和端粒結(jié)合蛋白的表達(dá)研究目的探討創(chuàng)傷后應(yīng)激障礙(post-traumatic stress disorder, PTSD)樣癥狀大鼠外周血白細(xì)胞端粒長(zhǎng)度的變化及海馬CA1區(qū)端粒結(jié)合蛋白的表達(dá)。方法取成年健康雄性Wistar大鼠42只,建立大鼠PTSD模型,應(yīng)用實(shí)時(shí)熒光定量PCR技術(shù)測(cè)量相對(duì)白細(xì)胞端粒長(zhǎng)度；免疫熒光技術(shù)檢測(cè)PTSD大鼠海馬CA1區(qū)TRF1和TRF2的表達(dá)變化。結(jié)果經(jīng)SPS刺激后大鼠端粒長(zhǎng)度縮短速率較對(duì)照組加快,有統(tǒng)計(jì)學(xué)差異(P0.05)。TRF1和TRF2蛋白的表達(dá)均隨著PTSD的發(fā)生表達(dá)增高,且在第14天表達(dá)最高,與對(duì)照組的表達(dá)有統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論P(yáng)TSD大鼠外周血白細(xì)胞端粒長(zhǎng)度縮短速率加快及TRF1和TRF2在海馬區(qū)表達(dá)增高,這與PTSD的病理發(fā)展和預(yù)后不良可能密切相關(guān)。第二部分經(jīng)SPS創(chuàng)傷后PTSD樣大鼠海馬CA1區(qū)IGF-1及其受體的表達(dá)研究目的研究胰島素樣生長(zhǎng)因子1(IGF-1)及其受體(IGF-1 receptor)在創(chuàng)傷后應(yīng)激障礙(post-traumatic stress disorder, PTSD)大鼠的海馬CA1區(qū)的表達(dá)。方法取成年健康雄性Wistar大鼠30只,采用國(guó)際認(rèn)定的SPS方法刺激建立大鼠PTSD模型,應(yīng)用免疫組化技術(shù)檢測(cè)PTSD大鼠海馬CA1區(qū)IGF-1和IGF-1 receptor的表達(dá)變化。結(jié)果經(jīng)SPS刺激后大鼠海馬CA1區(qū)IGF-1蛋白的表達(dá)隨著PTSD的發(fā)生表達(dá)增高,且在第14天表達(dá)最高,與正常對(duì)照組的表達(dá)有統(tǒng)計(jì)學(xué)差異(P0.05)。而IGF-1 receptor蛋白的表達(dá)在應(yīng)激前和應(yīng)激后的第1天、第7天其表達(dá)程度未見(jiàn)明顯差異(P0.05),而在第14天和第28天表達(dá)較前有所降低(P0.05)。結(jié)論應(yīng)激后IGF-1的表達(dá)增高,但其受體的表達(dá)卻沒(méi)有同步增高,甚至有所降低,這可能在一定程度上限制了IGF-1的積極作用,這可能參與了PTSD的認(rèn)知功能改變的病理生理學(xué)過(guò)程。
[Abstract]:Part one: expression of telomere and telomere binding protein in PTSD like rats after SPS trauma objective to investigate the changes of telomere length in peripheral white blood leukocytes and the expression of telomere binding protein in CA1 region of hippocampus in rats with post-traumatic stress disorder, PTSD) like symptoms after traumatic stress disorder. Methods 42 adult male Wistar rats were used to establish PTSD model. The telomere length of relative leukocytes was measured by real-time fluorescence quantitative PCR and the expression of TRF1 and TRF2 in the CA1 region of hippocampus of PTSD rats were detected by immunofluorescence technique. Results the shortening rate of telomere length of rats stimulated by SPS was faster than that of the control group. The expression of P0.05, TRF1 and TRF2 protein increased with the development of PTSD, and the highest expression was found on the 14th day, and there was a significant difference between the expression of TRF1 and the control group (P 0.05). Conclusion the increase of telomere length shortening rate and the expression of TRF1 and TRF2 in hippocampus of PTSD rats may be closely related to the pathological development and poor prognosis of PTSD. Part two: expression of IGF-1 and its receptors in the CA1 region of hippocampus of PTSD like rats after SPS trauma objective to study the expression of insulin-like growth factor (IGF-1) and its receptor (IGF-1 receptor) in the hippocampal CA1 region of post-traumatic stress disorder, PTSD) rats with post-traumatic stress disorder (PTSD). Methods the PTSD model was established in 30 adult healthy male Wistar rats stimulated by the internationally recognized SPS method. The expression of IGF-1 and IGF-1 receptor in the CA1 region of the hippocampus of PTSD rats was detected by immunohistochemical technique. Results after stimulation with SPS, the expression of IGF-1 protein in the CA1 area of hippocampus increased with the occurrence of PTSD, and the expression of IGF-1 protein was the highest on the 14th day, which was significantly different from that of the normal control group (P 0.05). However, there was no significant difference in the expression of IGF-1 receptor protein before and on the first day and the seventh day after stress, but on the 14th and 28th days, the expression of IGF-1 receptor protein was lower than that of the former. Conclusion the expression of IGF-1 increases after stress, but the expression of its receptor does not increase or even decrease, which may limit the positive effect of IGF-1 to some extent, which may be involved in the pathophysiological process of PTSD cognitive function change.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R749.5

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