本文選題：阿爾茨海默病 + 左卡尼汀��； 參考：《中國老年學(xué)雜志》2017年08期

【摘要】：目的比較左卡尼汀和多奈哌齊對腦室注射鏈脲佐菌素(STZ)致癡呆大鼠的治療作用。方法健康SD雄性大鼠32只,隨機(jī)分為對照組、模型組、左卡尼汀組、多奈哌齊組(n=8)。模型組和治療組在手術(shù)的第1、3天分別向兩側(cè)側(cè)腦室內(nèi)注射STZ 3 mg/kg,對照組給予相同體積的檸檬酸緩沖液。左卡尼汀組于手術(shù)10 d前給予左卡尼汀20 mg·kg~(-1)·d~(-1)灌胃,術(shù)后持續(xù)90 d,多奈哌齊組給予多奈哌齊0.45 mg·kg~(-1)·d~(-1)灌胃,其余兩組給予等量生理鹽水。術(shù)后第10、90天Morris水迷宮試驗(yàn),觀察大鼠學(xué)習(xí)記憶能力。術(shù)后8 w處死大鼠取海馬送電鏡觀察神經(jīng)元凋亡情況。結(jié)果術(shù)后10 d水迷宮測試,與對照組比較,模型組、左卡尼汀組、多奈哌齊組的潛伏期延長,過平臺次數(shù)下降(P0.01)。術(shù)后治療90 d,與模型組比較,多奈哌齊組的潛伏期縮短,過平臺次數(shù)增加(P0.01),左卡尼汀組潛伏期縮短(P0.05),過平臺次數(shù)無明顯增加(P0.05)。左卡尼汀組與多奈哌齊組比較各指標(biāo)差異有統(tǒng)計(jì)學(xué)意義(P0.01)。與模型組比較,兩治療組電鏡下觀察大鼠海馬細(xì)胞結(jié)構(gòu)細(xì)胞凋亡程度輕,以多奈哌齊治療組明顯。結(jié)論左卡尼汀與多奈哌齊都能減輕AD模型鼠海馬神經(jīng)元的凋亡,從而起到改善臨床癥狀的作用,左卡尼汀效果不如多奈哌齊。
[Abstract]:Aim to compare the therapeutic effects of levacarnitine and Donepezil on dementia rats induced by intraventricular injection of streptozotocin (STZ). Methods Thirty-two healthy male SD rats were randomly divided into control group, model group, L-carnitine group and Donepezil group. The model group and the treatment group were injected with STZ 3 mg / kg on the 1st day of operation respectively. The control group was given the same volume of citric acid buffer. The levacarnitine group was given levacarnitine 20 mg / kg / d ~ (-1) before operation for 90 days after operation, and the Donepezil group was given Donepezil 0.45 mg / kg / d ~ (-1) / d ~ (-1). The other two groups were given the same amount of normal saline. The ability of learning and memory was observed by Morris water maze test on day 10 ~ 90 after operation. The rats were killed 8 weeks after operation to observe the apoptosis of neurons by electron microscope. Results compared with the control group, the latencies of model group, L-carnitine group and Donepezil group were prolonged and the times of platform passing were decreased (P0.01). 90 days after operation, compared with the model group, the latency of Donepezil group was shortened, the times of platform crossing was increased (P 0.01), the latency of leucarnitine group was shortened (P 0.05), and the frequency of crossing platform was not significantly increased (P 0.05). Compared with Donepezil group, there were significant differences in every index between Levocarnitine group and Donepezil group (P 0.01). Compared with the model group, the apoptotic degree of hippocampal cells in the treatment group was less than that in the Donepezil group under electron microscope. Conclusion both levacarnitine and Donepezil can reduce the apoptosis of hippocampal neurons in AD model rats and thus improve the clinical symptoms. The effect of levacarnitine is not as good as that of Donepezil.
【作者單位】： 哈爾濱醫(yī)科大學(xué)附屬第四醫(yī)院神經(jīng)內(nèi)科;哈爾濱醫(yī)科大學(xué)附屬第四醫(yī)院老年病科;
【基金】：黑龍江省自然基金資助項(xiàng)目(No.0200869)
【分類號】：R749.16;R-332
，

本文編號：1811419