本文選題：絞股藍 + 血管性癡呆��； 參考：《遵義醫(yī)學(xué)院》2013年碩士論文

【摘要】：目的：探討中藥絞股藍對腦缺血再灌注所致的血管性癡呆小鼠海馬神經(jīng)元損傷、認知功能障礙的保護作用及機制。 方法：采用雙側(cè)頸總動脈阻斷(2VO)方法復(fù)制血管性癡呆小鼠模型,隨機將雄性C57BL6/G小鼠分為5組,即假手術(shù)(sham)組、血管性癡呆模型(VD)組、絞股藍治療組(低、中、高劑量組)。行為學(xué)Morris水迷宮檢測小鼠空間學(xué)習(xí)記憶能力、HE染色法觀察小鼠海馬神經(jīng)元的形態(tài)學(xué)變化、酶聯(lián)免疫分析法檢測小鼠海馬組織環(huán)磷酸腺苷(cAMP)的表達。 結(jié)果：①Morris水迷宮測試發(fā)現(xiàn)VD小鼠學(xué)習(xí)記憶能力下降；VD模型組小鼠的逃避潛伏期明顯比Sham組時間長(P0.01),絞股藍治療組小鼠逃避潛伏期明顯比VD模型組時間短(P0.05),絞股藍中、高劑量組逃避潛伏期明顯比低劑量組時間短(P0.05)；絞股藍中、高劑量組之間無顯著差異(P0.05)；VD模型組穿過平臺次數(shù)明顯少于Sham組(P0.01),VD模型組穿過平臺次數(shù)明顯少于GP低、中、高劑量組(P0.05),GP中、高劑量組穿過平臺次數(shù)明顯比低劑量組多(P0.05)。②HE染色顯示,Sham組CA1區(qū)海馬神經(jīng)元細胞數(shù)目多,排列整齊緊密,細胞核呈圓形或橢圓形；VD模型組小鼠海馬神經(jīng)元胞漿出現(xiàn)片狀壞死,海馬神經(jīng)細胞數(shù)目明顯減少,排列紊亂松散,細胞核固縮深染,細胞核邊界模糊。而GP中、高劑量組海馬神經(jīng)元細胞層次較清楚,排列較整齊,海馬神經(jīng)元細胞數(shù)量明顯增多,細胞核固縮深染現(xiàn)象少見。③與假手術(shù)組比較,VD模型組小鼠海馬組織cAMP含量明顯降低(P0.01),與VD模型組比較,絞股藍治療低、中、高劑量組小鼠海馬組織cAMP含量明顯增高(P0.01),與絞股藍治療低劑量組比較,絞股藍治療中、高劑量組小鼠海馬組織cAMP含量明顯增高(P0.05)。 結(jié)論：VD小鼠海馬區(qū)神經(jīng)元損傷和腦認知功能減退；中藥絞股藍對VD小鼠的神經(jīng)元損傷具有保護作用,增加海馬組織cAMP的表達,有利于改善VD小鼠的學(xué)習(xí)記憶能力。
[Abstract]:Aim: to investigate the protective effect and mechanism of gynostemma pentaphyllum on hippocampal neuron injury and cognitive dysfunction in vascular dementia mice induced by cerebral ischemia and reperfusion.Methods: the vascular dementia model was established by bilateral common carotid artery occlusion in mice. Male C57BL6/G mice were randomly divided into 5 groups: sham group, vascular dementia model group, gynostemma gynostemma group (low, middle and high dose group).Behavioral Morris water maze was used to detect the spatial learning and memory ability of mice. The morphological changes of hippocampal neurons in mice were observed by HE staining and the expression of cyclic adenosine monophosphate (camp) was detected by enzyme-linked immunosorbent assay (Elisa).Results the escape latency of VD mice was significantly longer than that of Sham group (P 0.01), the escape latency of gynostemma treated group was shorter than that of VD model group (P 0.05), and that of gynostemma pentaphyllum group was lower than that of VD model group.The escape latency of high dose group was significantly shorter than that of low dose group (P 0.05), in gynostemma pentaphyllum, there was no significant difference between high dose group and high dose group. The number of passing through platform in high dose group was significantly less than that in Sham group (P 0.01) and the number of crossing platform in VD group was significantly less than that in GP.In the high dose group (P 0.05), the number of neurons passing through the plateau in the high dose group was significantly higher than that in the low dose group, and the number of hippocampal neurons in the CA1 area of the Sham group was more than that in the low dose group.In the group of round or elliptical VD model, the neurons in the hippocampus showed lamellar necrosis, the number of neurons in the hippocampus decreased obviously, the number of neurons in the hippocampus was loosely arranged, the nuclei were pyknotic and deep stained, and the nuclear boundaries were blurred.In GP group, the level of hippocampal neurons was clear and arranged neatly, and the number of hippocampal neurons increased obviously.Compared with sham-operated group, the cAMP content of hippocampus in VD model group was significantly lower than that in VD model group, and that in VD model group was lower than that in VD model group.The content of cAMP in hippocampus of high dose group was significantly higher than that of low dose group of gynostemma pentaphyllum. The content of cAMP in hippocampus of high dose group was significantly higher than that of low dose group of gynostemma pentaphyllum.Conclusion gynostemma pentaphyllum has a protective effect on neuron damage in VD mice, increases the expression of cAMP in hippocampus, and improves the learning and memory ability of VD mice.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R285.5;R749.13

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