本文選題：慢性腦低灌注　切入點：肢體遠隔缺血適應(yīng)　出處：《山東醫(yī)藥》2017年24期

【摘要】：目的探討重復(fù)肢體遠隔缺血適應(yīng)(LRIC)對慢性腦低灌注大鼠空間學(xué)習(xí)記憶的影響及作用機制。方法選擇健康雄性SD大鼠60只,隨機均分為假手術(shù)組、模型組、單次LRIC組、多次LRIC組,后三組采用雙側(cè)頸總動脈結(jié)扎(2VO)法建立大腦低灌注損傷模型,假手術(shù)組僅分離雙側(cè)勁總動脈不予結(jié)扎。術(shù)后3天時,單次LRIC組進行1次LRIC,具體方法為采用紗布條綁扎雙側(cè)下肢完全阻斷股動脈血流,綁扎10 min,松開10 min,每次3個循環(huán);多次LRIC組每天進行LRIC 1次,連續(xù)6周。LRIC結(jié)束后4周采用Morris水迷宮試驗檢測逃避潛伏期及目標象限時間百分比,激光多普勒血流儀檢測海馬CA1區(qū)腦血流,電鏡觀察海馬CA1區(qū)軸索及血管的超微結(jié)構(gòu)。結(jié)果假手術(shù)組、多次LRIC組逃避潛伏期均短于模型組和單次LRIC組,目標象限時間百分比均長于模型組和單次LRIC組,組間比較P0.05或0.01。假手術(shù)組、模型組、單次LRIC組、多次LRIC組海馬CA1區(qū)相對腦血流分別為100%、(61.3±11.7)%、(59.5±13.1)%、(80.2±14.6)%,假手術(shù)組、多次LRIC組均高于模型組和單次LRIC組(P均0.05)。模型組海馬CA1區(qū)血管內(nèi)皮不光滑、基底膜增厚、周圍明顯水腫,軸索明顯斷裂、板層分離;多次LRIC組上述改變明顯減輕。結(jié)論重復(fù)LRIC可改善慢性腦血流低灌注導(dǎo)致的認知功能障礙;增加海馬區(qū)血供、抑制海馬區(qū)微血管損傷可能是其作用機制。
[Abstract]:Objective to investigate the effect and mechanism of repeated limb remote ischemic adaptation (LRICC) on spatial learning and memory in rats with chronic cerebral hypoperfusion.Methods 60 male Sprague-Dawley rats were randomly divided into three groups: sham operation group, model group, single LRIC group, multiple LRIC group, and the latter three groups were treated with bilateral common carotid artery ligation.In the sham operation group, bilateral common arteries were separated and not ligated.On the 3rd day after operation, the single LRIC group was performed once. The specific method was to completely block the femoral artery blood flow in the lower extremities of both sides by using gauze bandage, ligation for 10 mins, release for 10 mins, and 3 cycles for each time, and LRIC was performed once a day in the LRIC group several times a day.The escape latency and the percentage of target quadrant time were measured by Morris water maze test, the cerebral blood flow in CA1 area of hippocampus was measured by laser Doppler flow analyzer, and the ultrastructure of axons and blood vessels in CA1 region of hippocampus were observed by electron microscope at 4 weeks after the end of LRIC.Results the escape latency of sham operation group, multiple LRIC group was shorter than that of model group and single LRIC group, and the percentage of target quadrant time was longer than that of model group and single LRIC group (P0.05 or 0.01).In the sham operation group, model group, single LRIC group, multiple LRIC group, the relative cerebral blood flow of the hippocampal CA1 area was 61.3 鹵11.7 and 59.5 鹵13.1 respectively. The sham operation group, multiple LRIC group were higher than the model group and the single LRIC group (P 0.05).In the model group, vascular endothelium was not smooth, basement membrane thickened, peripheral edema, axonal rupture and lamellar separation were observed in the hippocampal CA1 area, and the above changes were significantly alleviated in the multiple LRIC group.Conclusion repeated LRIC can improve the cognitive dysfunction induced by chronic cerebral blood flow hypoperfusion and increase the blood supply in the hippocampus and inhibit the microvascular injury in the hippocampus.
【作者單位】： 濰坊醫(yī)學(xué)院;首都醫(yī)科大學(xué)低氧醫(yī)學(xué)研究所;
【基金】：國家自然科學(xué)基金資助項目(81573867) 首都衛(wèi)生發(fā)展科研專項資助項目(首發(fā)2011-1001-03)
【分類號】：R749.1

【相似文獻】

相關(guān)期刊論文 前3條

1 李玉梅;劉雨;張婷;付劍亮;;糖尿病加重慢性腦低灌注大鼠空間學(xué)習(xí)記憶能力損害及機制探討[J];中國現(xiàn)代神經(jīng)疾病雜志;2014年06期

2 張敏慧;韓彥青;王曉暉;裴宇恒;李東芳;李光來;;犬尿氨酸通路相關(guān)物質(zhì)在大鼠慢性腦低灌注致認知障礙中的作用[J];中華老年心腦血管病雜志;2014年06期

3 ;[J];;年期

相關(guān)會議論文 前1條

1 趙玲;劉麗;李雅莉;張麗;張?zhí)m;李林;;慢性腦低灌注狀態(tài)對大鼠學(xué)習(xí)記憶功能及腦組織自由基代謝、乙酰膽堿酯酶活力的影響[A];中國神經(jīng)科學(xué)學(xué)會第六屆學(xué)術(shù)會議暨學(xué)會成立十周年慶祝大會論文摘要匯編[C];2005年

相關(guān)博士學(xué)位論文 前1條

1 楊英;低氧誘導(dǎo)因子1α對慢性腦低灌注大鼠的內(nèi)源性神經(jīng)保護作用研究[D];武漢大學(xué);2016年

相關(guān)碩士學(xué)位論文 前3條

1 王敏;吲哚胺2，，3-二加氧酶抑制劑對慢性腦低灌注致認知障礙大鼠行為學(xué)的改善作用[D];山西醫(yī)科大學(xué);2015年

2 范志榮;IDO抑制劑對慢性腦低灌注大鼠海馬CA1區(qū)細胞凋亡的影響[D];山西醫(yī)科大學(xué);2016年

3 席燁;慢性腦低灌注致大鼠認知功能障礙及其相關(guān)因素的研究[D];第四軍醫(yī)大學(xué);2014年

本文編號：1719808