本文關鍵詞： Aβ 氧化損傷 PC細胞 亞硒酸鈉 丙二醛(MDA)　出處：《中國老年學雜志》2014年23期 　論文類型：期刊論文

【摘要】：目的研究β淀粉樣蛋白(Aβ)對PC12細胞的氧化損傷以及亞硒酸鈉的保護作用。方法將PC12細胞分為正常對照組、亞硒酸鈉預處理組、亞硒酸鈉預處理后損傷組、損傷組,觀察細胞形態(tài)、用MTT法檢測細胞存活率,流式細胞術檢測凋亡,測定各組乳酸脫氫酶(LDH)漏出率、丙二醛(MDA)含量及谷胱甘肽過氧化物酶(GSH-Px)活性。結果 Aβ抑制PC12細胞的分裂增殖,經(jīng)過Aβ作用24 h的PC12細胞,其GSH-Px活性下降,MDA含量升高;一定濃度亞硒酸鈉預處理可有效地減輕Aβ引起的PC12細胞損傷,與Aβ?lián)p傷組相比亞硒酸鈉預處理后Aβ?lián)p傷組的細胞存活率增加、凋亡下降、GSH-Px活性增高、LDH漏出率、MDA含量下降。結論亞硒酸鈉對Aβ引起的PC12細胞氧化損傷具有明顯的保護作用。
[Abstract]:Objective to study the amyloid beta (A beta) on oxidative damage of PC12 cells and the protective effect of sodium selenite. Methods PC12 cells were divided into normal control group, sodium selenite pretreatment group, injury group, injury group, pretreatment of sodium selenite, observe cell morphology, cell survival rate detected by MTT method detection of apoptosis, flow cytometry, measured by lactate dehydrogenase (LDH) leakage rate, malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity. The results of A beta inhibition of PC12 cell proliferation by A beta PC12 cells 24 h, decreased the GSH-Px activity, MDA content increased; PC12 cell injury a certain concentration of sodium selenite pretreatment can effectively reduce the A caused by beta, compared with the injury group A beta pretreatment of sodium selenite after injury group A beta increased the cell survival rate, apoptosis decreased, GSH-Px activity increased, the leakage rate of LDH, MDA content decreased. Conclusion sodium selenite on A The oxidative damage of PC12 cells induced by beta has obvious protective effect.

【作者單位】： 西安醫(yī)學院第一附屬醫(yī)院神經(jīng)內(nèi)科;
【基金】：陜西省教育廳專項科研計劃項目(2010JK808) 西安醫(yī)學院科研基金青年項目(2007QN02) 西安醫(yī)學院附屬醫(yī)院科研基金項目(XYFY08-08)
【分類號】：R749.16
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本文編號：1522782