發(fā)布時(shí)間：2018-02-12 03:01

  本文關(guān)鍵詞： 妊娠期應(yīng)激 慢性應(yīng)激 促腎上腺皮質(zhì)激素釋放激素 抑郁 維生素D　出處：《安徽醫(yī)科大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的1.探討妊娠期慢性應(yīng)激對(duì)不同性別子代小鼠抑郁程度的影響及其可能機(jī)制。2.通過(guò)給予維生素D,研究維生素D對(duì)妊娠期慢性應(yīng)激促進(jìn)子代雄鼠應(yīng)激致抑郁是否有抑制作用。方法以1個(gè)月齡的子代昆明小鼠為研究對(duì)象,實(shí)驗(yàn)小鼠分為妊娠期正常處理-子代正常處理(control)組、妊娠期慢性應(yīng)激-子代正常處理(CPS)組、妊娠期正常處理-子代慢性應(yīng)激(COS)組、妊娠期慢性應(yīng)激-子代慢性應(yīng)激(COS+CPS)組。通過(guò)強(qiáng)迫游泳和懸尾實(shí)驗(yàn)觀察子代小鼠的抑郁程度;通過(guò)HE染色觀察子代小鼠海馬CA 3區(qū)神經(jīng)元病理組織形態(tài)變化;Western blot法檢測(cè)小鼠海馬中雷帕霉素靶蛋白(m TOR)的表達(dá)量;ELISA法檢測(cè)小鼠血清中促腎上腺皮質(zhì)激素釋放激素(CRH)的含量。在以上實(shí)驗(yàn)基礎(chǔ)上,以1個(gè)月齡的子代昆明雄鼠為研究對(duì)象,隨機(jī)分為妊娠期慢性應(yīng)激-子代慢性應(yīng)激(COS+CPS)組和妊娠期慢性應(yīng)激-子代慢性應(yīng)激-VD灌胃(COS+CPS+VD)組,通過(guò)強(qiáng)迫游泳實(shí)驗(yàn)、懸尾實(shí)驗(yàn)、曠場(chǎng)實(shí)驗(yàn)和糖水偏好實(shí)驗(yàn)等行為學(xué)實(shí)驗(yàn)檢測(cè)子代小鼠的抑郁程度;通過(guò)觀察海馬CA3區(qū)病理、m TOR表達(dá)量和血清CRH含量進(jìn)行抗抑郁的機(jī)制研究。結(jié)果1.強(qiáng)迫游泳結(jié)果顯示,與對(duì)照組相比,子代雄鼠CPS和COS組不動(dòng)時(shí)間延長(zhǎng)(P0.05),COS+CPS組不動(dòng)時(shí)間較COS組明顯延長(zhǎng)(P0.05);子代雌鼠COS組不動(dòng)時(shí)間延長(zhǎng)(P0.05),COS+CPS組不動(dòng)時(shí)間未進(jìn)一步改變。2.懸尾實(shí)驗(yàn)結(jié)果顯示,與對(duì)照組相比,子代雄鼠CPS和COS組不動(dòng)時(shí)間延長(zhǎng)(P0.05),COS+CPS組不動(dòng)時(shí)間較COS組明顯延長(zhǎng)(P0.05);子代雌鼠COS組不動(dòng)時(shí)間延長(zhǎng)(P0.05),COS+CPS組不動(dòng)時(shí)間未進(jìn)一步改變。3.HE染色結(jié)果顯示,與對(duì)照組相比,子代雄鼠COS組海馬CA 3區(qū)神經(jīng)元細(xì)胞損傷的數(shù)目明顯增加,神經(jīng)元排列疏松、紊亂,核固縮、濃染,COS+CPS組海馬CA 3區(qū)神經(jīng)元進(jìn)一步損傷;子代雌鼠四組之間未見(jiàn)明顯差異。4.WB結(jié)果顯示,與對(duì)照組相比,子代雄鼠COS組海馬組織中m TOR表達(dá)量減少(P0.05),COS+CPS組m TOR表達(dá)量較COS組進(jìn)一步減少(P0.05);子代雌鼠COS組海馬組織中m TOR表達(dá)量減少(P0.05),COS+CPS組m TOR表達(dá)量較COS組未見(jiàn)進(jìn)一步減少。5.ELISA結(jié)果顯示,與對(duì)照組相比,子代雄鼠COS組血清中CRH濃度升高(P0.05),COS+CPS組血清CRH濃度較COS組進(jìn)一步升高(P0.05);子代雌鼠四組之間未見(jiàn)上述變化。6.強(qiáng)迫游泳和懸尾實(shí)驗(yàn)結(jié)果顯示,COS+CPS+VD組子鼠不動(dòng)時(shí)間較COS+CPS組減少(P0.05)。7.糖水偏好實(shí)驗(yàn)結(jié)果顯示,糖水偏好實(shí)驗(yàn)顯示,CPS+COS+VD組小鼠糖水消耗量、糖水偏好百分比和平均體重糖水消耗明顯多于CPS+COS組(P0.05),CPS+COS+VD組純水消耗量小于COS+CPS組(P0.05)。8.曠場(chǎng)實(shí)驗(yàn)結(jié)果顯示,兩組小鼠在運(yùn)動(dòng)長(zhǎng)度、平均速度、穿越象限次數(shù)、中央格進(jìn)入次數(shù)、中央格停留時(shí)間、糞便粒數(shù)均無(wú)統(tǒng)計(jì)學(xué)差異(P0.05),在站立次數(shù)上,CPS+COS+VD組較CPS+COS組增多,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。9.HE染色結(jié)果顯示,加維生素D組CA3區(qū)神經(jīng)元排列緊密,神經(jīng)元數(shù)目增多,核濃染固縮少;對(duì)照組。10.WB結(jié)果顯示,CPS+COS+VD組的m TOR蛋白的表達(dá)量較CPS+COS組明顯升高(P0.05)。11.ELISA結(jié)果顯示,CPS+COS+VD組血清中CRH濃度較CPS+COS組降低(P0.05)。結(jié)論1.妊娠期慢性應(yīng)激進(jìn)一步加劇慢性應(yīng)激所致的子代雄鼠抑郁,其機(jī)制可能與其升高小鼠血清CRH水平,抑制m TOR的表達(dá),最終引起海馬CA 3區(qū)神經(jīng)元損傷有關(guān)。妊娠期慢性應(yīng)激未能進(jìn)一步加劇子代雌鼠應(yīng)激所致的抑郁程度。2.維生素D在妊娠期慢性應(yīng)激進(jìn)一步加劇子代應(yīng)激致抑郁的過(guò)程中起到保護(hù)作用。
[Abstract]:Objective to investigate the 1. pregnancy effects of chronic stress on the sexual differentiation of mice and the possible mechanism of.2. depression by administration of vitamin D, vitamin D on pregnancy chronic stress in male offspring rats of stress induced depression is inhibited. Methods 1 month old mice offspring in Kunming as the research object, the experiment of mice divided into normal pregnancy treated offspring normal treatment (control) group, chronic stress - offspring of normal pregnancy (CPS) treatment group, normal pregnancy treatment - offspring of chronic stress (COS) group, chronic stress and chronic stress in offspring of pregnancy (COS+CPS) group. The degree of depression and forced swimming the tail suspension test in mice offspring observation; observation of hippocampal CA 3 neurons with HE staining pathological and morphological changes; rapamycin target protein to detect the expression of the Western blot method (m TOR) the expression of ELISA in serum was detected; 涓績(jī)鑲句笂鑵虹毊璐ㄦ縺绱犻噴鏀炬縺绱，

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