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  本文關(guān)鍵詞：涉及單胺遞質(zhì)代謝的抑郁癥性差異及其自殺行為研究　出處：《重慶醫(yī)科大學》2014年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 慢性不可預(yù)見性刺激 轉(zhuǎn)運體 單胺氧化酶B 性別差異 自殺行為

【摘要】：目的： 通過慢性不可預(yù)見刺激（CUS）致大鼠抑郁行為探討性別差異與神經(jīng)細胞單胺氧化酶B（MAO-B）、5-羥色胺轉(zhuǎn)運體（5-HTT）及去甲腎上腺素轉(zhuǎn)運體（NET）表達的關(guān)系。結(jié)合臨床具有自殺行為的抑郁患者，探討其自殺行為是否存在性別差異。 方法： 1. SD大鼠雌雄兩性各30只，隨機分為雄對照組（MCG）、雄模型組（MMG）、雌對照組（FCG）和雌模型組（FMG）,采用孤養(yǎng)結(jié)合CUS方式建立大鼠抑郁模型。 2.開場實驗和強迫游泳實驗評價大鼠焦慮抑郁行為。 3. HE染色鏡下觀察大鼠海馬病理形態(tài)改變。 4.Western blot（WB）法和熒光定量PCR法分別測定腦橋NET和海馬5-HTT、MAO-B蛋白及mRNA表達。 5.計算機檢索中國知網(wǎng)學術(shù)期刊數(shù)據(jù)庫、Pubmed等中英文數(shù)據(jù)庫，獲得抑郁癥致男女自殺行為的相關(guān)文獻。依照一定標準進行資料篩選并將合格資料進行Meta分析。 結(jié)果： 1.經(jīng)CUS處理的FMG和MMG大鼠開場實驗水平得分和垂直得分顯著低于各自對照組，，且MMG大鼠水平得分、垂直得分顯著低于FMG；FMG和MMG大鼠強迫游泳不動時間顯著長于各自對照組，且MMG大鼠不動時間顯著長于FMG。 2.MMG、FMG組大鼠海馬細胞有明顯的核固縮、深染等損傷現(xiàn)象，F(xiàn)MG組損傷程度似不如MMG組嚴重。 3.與各自對照組相比，F(xiàn)MG、MMG大鼠5-HTT表達均下降，NET和MAO-B表達均升高；且MMG大鼠腦橋NET表達顯著高于FMG大鼠。 4.臨床資料的Meta分析結(jié)果顯示，男性與女性總自殺行為比值比（odds ratio，OR）為0.84（95%CI:0.63—1.12，P=0.23）。自殺死亡的OR值為0.94（95%CI:0.18—5.07，P=0.94），自殺未遂OR值為0.75（95%CI:0.59—0.95,P=0.02）。 結(jié)論： CUS致大鼠抑郁行為存在顯著的性別差異，此差異可能與影響單胺遞質(zhì)代謝的MAO-B、NET、5-HTT表達量的變化不同有關(guān)。男女抑郁癥患者自殺行為存在一定性別差異，男性自殺未遂的發(fā)生率顯著高于女性。
[Abstract]:Objective: To explore the relationship between sex difference and neuronal monoamine oxidase (BMAO-B-) in depression induced by chronic unpredictable stimulation (CUS) in rats. The relationship between serotonin transporter (5-HTT) and norepinephrine transporter (NET). Methods: 1. Thirty male and female SD rats were randomly divided into male control group (MCG), male model group (MMGN), female control group (FCGG) and female model group (FMG). The depression model of rats was established by using solitary nourishment combined with CUS. 2. The behavior of anxiety and depression in rats was evaluated by open field experiment and forced swimming experiment. 3. The histopathological changes of hippocampus were observed by HE staining. 4. The expression of MAO-B protein and mRNA in pons NET and hippocampus were detected by Western blottle and fluorescence quantitative PCR, respectively. 5. Computer retrieval of Chinese and English databases such as online academic journals. To obtain the relevant literature on suicidal behavior of men and women with depression. To screen the data according to certain criteria and to analyze the qualified data by Meta. Results: 1. The open field test level and vertical score of FMG and MMG rats treated with CUS were significantly lower than those of the control group, and the horizontal score and vertical score of MMG rats were significantly lower than those of the control group. The immobility time of FMG and MMG rats was significantly longer than that of their control groups, and the immobility time of MMG rats was significantly longer than that of FMGs. 2. The damage degree of hippocampal cells in MMG- FMG group was not as serious as that in MMG group. 3.Compared with the control group, the expression of 5-HTT decreased and the expression of net and MAO-B increased in MMG rats. The expression of NET in MMG rats was significantly higher than that in FMG rats. 4. The Meta analysis of clinical data showed that the ratio of total suicide behavior between male and female was 0.8495 CI: 0.63-1.12. The OR value of suicide death was 0.94% 95% CI: 0.18-5.07% P0.94). The OR value of attempted suicide was 0.75% 95% CI: 0.59-0.95% P0.02. Conclusion: There were significant gender differences in depressive behaviors induced by CUS in rats, which may be related to MAO-BN, which affects the metabolism of monoamine transmitters. The changes of 5-HTT expression were related to different levels of 5-HTT expression. There were some gender differences in suicidal behavior between male and female patients with depression, and the incidence of attempted suicide in male was significantly higher than that in female.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R749.4

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本文編號：1375897