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  本文關(guān)鍵詞：TREM2作為阿爾茨海默病潛在生物學標志物的研究　出處：《青島大學》2014年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 阿爾茨海默病 TREM2 生物學標記 實時熒光定量RT-PCR法 流式細胞儀 酶聯(lián)免疫吸附試驗

【摘要】：目的：本研究旨在探討髓樣細胞2觸發(fā)受體(TREM2)作為阿爾茨海默病(AD)診斷生物學標志的潛在價值。 方法：采用病例對照研究,選取年齡和性別相匹配的北方漢族人群的116例散發(fā)阿爾茨海默病患者和116例健康人為研究對象。應用實時熒光定量(RT-PCR)檢測外周血單個核細胞(單核細胞和淋巴細胞)中TREM2mRNA水平,應用流式細胞儀(FCM)和酶聯(lián)免疫實驗(ELISA)對外周血細胞和血漿中TREM2蛋白水平的表達差異進行研究；應用參數(shù)檢驗等統(tǒng)計學方法進行分析；應用受試者工作特征曲線(ROC)對TREM2作為AD診斷的特異性和靈敏度進行分析。 結(jié)果：TREM2mRNA在AD患者和健康對照組外周血單個核細胞(PBMCs)中表達水平差異有統(tǒng)計學意義：AD組/對照組：0.69±0.25/0.60±0.17,p=0.002；TREM2蛋白在單核細胞上表達水平差異有統(tǒng)計學意義：AD組/對照組：41.28±15.72%/27.00±15.28%,p0.001；TREM2蛋白在中性粒細胞和血漿中在AD組中的表達較健康對照組高(38.36±21.33%versus34.56±20.19%,p=0.19：20.97±7.25ng/Lversus19.63±6.84ng/L,p=0.154),雖然這種差異未達到統(tǒng)計學意義；此外,我們發(fā)現(xiàn)TREM2在淋巴細胞上不表達。在AD組中,TREM2mRNA水平與簡易精神狀態(tài)量表(MMSE)評分之間存在顯著的相關(guān)性(r=-0.482,p0.01),TREM2蛋白水平與MMSE評分之間亦存在顯著相關(guān)性(r=-0.582,p0.01)；采用ROC曲線對TREM2mRNA水平進行分析,TREM2mRNA的曲線下面積為0.596,其在AD診斷中靈敏度和特異度分別為56%、55%；采用ROC曲線對AD組和對照組單核細胞上TREM2蛋白表達水平差異進行分析,TREM2在AD診斷中的敏感度和特異度為68%和72%,診斷準確率為70%。 結(jié)論：研究發(fā)現(xiàn)TREM2在AD患者PBMCs中mRNA及蛋白的表達水平均較健康人表達升高,雖然,TREM2不能作為獨立的AD的生物學標記,但聯(lián)合其他指標能提高TREM2的診斷價值。
[Abstract]:Objective: to investigate the potential value of myeloid cell 2 trigger receptor (TREM2) as a diagnostic marker for Alzheimer's disease (AD). Methods: a case-control study was conducted. A total of 116 sporadic Alzheimer's disease patients and 116 healthy persons were selected from Han nationality population of northern China matched by age and sex. Real-time fluorescence quantitative analysis (RT-PCR) was used. The levels of TREM2mRNA in peripheral blood mononuclear cells (monocytes and lymphocytes) were measured. Flow cytometry (FCM) and enzyme-linked immunosorbent assay (Elisa) were used to study the expression of TREM2 protein in peripheral blood cells and plasma. Statistical methods such as parameter test were used to analyze; The specificity and sensitivity of TREM2 in the diagnosis of AD were analyzed by using the operating characteristic curve of subjects. Results the expression of TREM2 mRNA in PBMCsof peripheral blood mononuclear cells (PBMC) in AD patients and healthy controls. There was significant difference in the expression level between the two groups. The ratio of the two groups was 0.69 鹵0.25 / 0.60 鹵0.17. P0. 002; The expression level of TREM2 protein in monocytes was significantly different from that in the control group and the control group: 41.28 鹵15.72% / 27.00 鹵15.28p 0.001; The expression of TREM2 protein in neutrophils and plasma was 38.36 鹵21.33 in AD group and 34.56 鹵20.19% in control group. P: 0.1920.97 鹵7.25ng / L versus 19.63 鹵6.84ng / L = 0.154ng / L, although the difference was not statistically significant; In addition, we found that TREM2 was not expressed on lymphocytes. There was a significant correlation between TREM2mRNA level and MMSE score. There was also a significant correlation between TREM2 protein level and MMSE score. The area under the curve of TREM2mRNA level analysis by ROC curve was 0.596. The sensitivity and specificity of TREM2 in AD diagnosis were 56%, respectively. 55. The difference of TREM2 protein expression between AD group and control group was analyzed by ROC curve. The sensitivity and specificity of TREM2 in AD diagnosis were 68% and 72% respectively. The diagnostic accuracy was 70. Conclusion: the expression of mRNA and protein in PBMCs of AD patients was higher than that of healthy controls, although TREM2 could not be used as an independent marker of AD. But combined with other indicators can improve the diagnostic value of TREM2.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R749.16

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本文編號：1374288