本文關(guān)鍵詞：丁苯酞改善血管性癡呆大鼠認(rèn)知功能及其對(duì)海馬區(qū)GAP-43和突觸素表達(dá)的影響　出處：《中國(guó)老年學(xué)雜志》2016年08期 　論文類型：期刊論文

  更多相關(guān)文章： 丁苯酞 血管性癡呆 認(rèn)知功能 GAP- 突觸素P

【摘要】：目的探討丁苯酞對(duì)血管性癡呆(VD)大鼠認(rèn)知功能及對(duì)海馬區(qū)GAP-43和突觸素P38表達(dá)的影響。方法選取雄性Wistar大鼠90只,隨機(jī)分為觀察組、對(duì)照組、空白組各30只�？瞻捉M不采取任何操作;對(duì)照組大鼠制成VD模型,并予以生理鹽水;觀察組大鼠制成VD模型后,予以丁苯肽注射液。治療1個(gè)月后,采取MG-Y-型迷宮檢測(cè)其認(rèn)知功能以及采用Western印跡和RT-PCR分別檢測(cè)大鼠海馬區(qū)內(nèi)GAP-43和突觸素P38的表達(dá)。結(jié)果觀察組和空白組大鼠的錯(cuò)誤反應(yīng)次數(shù)均明顯低于對(duì)照組(P均0.05),且空白組大鼠降低更明顯(P0.05)。對(duì)照組和觀察組的GAP-43在大鼠海馬區(qū)內(nèi)的mRNA中表達(dá)較空白組高(P均0.05),且觀察組表達(dá)程度較對(duì)照組更高(P0.05);觀察組的突觸素P38在大鼠海馬區(qū)內(nèi)的mRNA中表達(dá)較空白組和對(duì)照組均提高(P均0.05),而對(duì)照組和空白組之間表達(dá)無(wú)差異(P0.05);觀察組GAP-43在大鼠海馬區(qū)內(nèi)的蛋白中表達(dá)較對(duì)照組和空白組均提高(P均0.05),對(duì)照組和空白組之間表達(dá)無(wú)差異(P0.05);對(duì)照組和觀察組的突觸素P38在大鼠海馬區(qū)內(nèi)的蛋白中表達(dá)較空白組均提高(P均0.05),且觀察組表達(dá)程度較對(duì)照組更高(P0.05)。結(jié)論對(duì)于VD大鼠,可予以丁苯酞注射液治療,其可促進(jìn)神經(jīng)元的修復(fù)及再生,改善VD大鼠海馬區(qū)GAP-43及突觸素P38的表達(dá),從而提供其認(rèn)知功能,改善其預(yù)后。
[Abstract]:Objective to investigate the butylphthalide on vascular dementia (VD) cognitive function of rats and its effect on the expression of GAP-43 in hippocampus and synaptophysin P38. Methods 90 male Wistar rats were selected and randomly divided into the observation group, the control group and the blank group of 30 rats. The blank group did not take any operation; the rats in the control group made the VD model and given the physiological saline; the rats of the observation group were made into the VD model and gave the butadiepin injection. After 1 months of treatment, the expression by MG-Y- maze test and the cognitive function by Western blot and RT-PCR were detected in rat hippocampal area GAP-43 and synaptophysin P38. Results the number of erroneous responses in the observation group and the blank group was significantly lower than that in the control group (P 0.05), and the decrease in the blank group was more obvious (P0.05). The observation group and the control group of GAP-43 mRNA in hippocampus in rats expressed higher than those in control group (P 0.05), and the expression level of the observation group higher than the control group (P0.05); the observation group of synapsin P38 expression compared with control group and control group in the mRNA hippocampus in rats were increased (P 0.05), and between the control group and the blank group was no significant difference (P0.05); the observation group in GAP-43 protein in hippocampus of rats in the expression were higher than those in the control group and the control group (P 0.05), between the control group and the blank group was no significant difference (P0.05); the control group and the observation the P38 group than the control group significantly increased synaptophysin expression in hippocampus in protein in rats (P 0.05), and the expression level of the observation group higher than the control group (P0.05). Conclusion the VD rats can be butylphthalide injection therapy, which can promote the repair and regeneration of neurons, improve the expression of VD in rat GAP-43 and synaptophysin P38 in hippocampus, thus providing the cognitive function, improve the prognosis.
【作者單位】： 齊齊哈爾醫(yī)學(xué)院附屬第三醫(yī)院神經(jīng)內(nèi)科;齊齊哈爾醫(yī)學(xué)院生物化學(xué)與分子生物學(xué)教研室;哈爾濱醫(yī)科大學(xué)第一臨床醫(yī)學(xué)院心內(nèi)科;
【基金】：黑龍江衛(wèi)生廳科研基金資助項(xiàng)目(項(xiàng)目編號(hào):HD2014015)
【分類號(hào)】：R749.13
【正文快照】： 血管性癡呆(VD)的發(fā)病率近年來(lái)持續(xù)上升,現(xiàn)已成為中老年人常見(jiàn)病之一,在我國(guó)其患病率已達(dá)2.1%~3.5%〔1,2〕。而目前針對(duì)VD的治療方法主要是通過(guò)促進(jìn)腦部微循環(huán),改善血液灌注等對(duì)癥治療。丁苯酞注射液可以改善腦卒中患者的中樞神經(jīng)系統(tǒng)損傷以及腦能量代謝和腦缺血區(qū)的微循環(huán)。

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳同度,張昌穎;素食大鼠的貧血現(xiàn)象[J];營(yíng)養(yǎng)學(xué)報(bào);1957年04期

2 陳偉強(qiáng);趙善廣;;自制注射用大鼠固定裝置[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);1992年04期

3 肖柳英,林培英,馮昭明,張丹;不同周齡的SD大鼠生理、生化及體重的正常值測(cè)定[J];中藥新藥與臨床藥理;1996年03期

4 李淑云;簡(jiǎn)易大鼠灌胃器的制作[J];錦州醫(yī)學(xué)院學(xué)報(bào);2001年04期

5 楊明智,陳積圣;一種大鼠抓取與固定的新工具介紹[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2001年03期

6 戴英,陸群;復(fù)方H_(505)對(duì)Wistar大鼠外周血的血液流變學(xué)指標(biāo)的影響[J];中國(guó)血液流變學(xué)雜志;2001年01期

7 韋應(yīng)波,孫喜慶,曹新生,姚永杰,馮岱雅,楊長(zhǎng)斌;+Gz暴露時(shí)間對(duì)大鼠記憶功能和行為的影響[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2003年01期

8 呂學(xué)軍,郭俊生,李敏,周利梅,張永娟;暈船大鼠體內(nèi)鐵含量的變化[J];中國(guó)職業(yè)醫(yī)學(xué);2003年04期

9 湯仁仙,王迎偉,王慧,周峰;201A中藥合劑對(duì)大鼠抗腎小球基底膜腎炎病變的影響[J];徐州醫(yī)學(xué)院學(xué)報(bào);2003年06期

10 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡(jiǎn)易的大鼠保定方法[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2004年01期

相關(guān)會(huì)議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對(duì)大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國(guó)顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會(huì)論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號(hào)：1340877