【摘要】：目的:觀察不同劑量糖皮質(zhì)激素(GC)對(duì)體外培養(yǎng)成骨細(xì)胞活性、分化的影響以及牛膝活性成分β蛻皮甾酮(β-Ecd)的干預(yù)作用,以探討GC引起骨質(zhì)疏松的部分機(jī)制。方法:由骨髓基質(zhì)細(xì)胞分化培養(yǎng)成骨細(xì)胞,用地塞米松(Dex)以不同劑量不同作用時(shí)間處理細(xì)胞,MTT法測(cè)定細(xì)胞活性后,將細(xì)胞分為7組:正常對(duì)照組、1×10-5mol/L GC模型組、GC+10-5mol/L RU486組、GC+10-5mol/L阿侖膦酸鈉(ALN)組、GC+10-5mol/Lβ-Ecd組、GC+10-6mol/Lβ-Ecd組、GC+10-7mol/Lβ-Ecd組。Real time PCR檢測(cè)相關(guān)基因mRNA表達(dá),Western Blot檢測(cè)β-catenin蛋白表達(dá)。結(jié)果:小劑量濃度Dex(≤10-8mol/L)有促進(jìn)成骨細(xì)胞活性的趨勢(shì),而超生理劑量的Dex則抑制細(xì)胞的分化與活性。GC模型組GR、RunX2、OP-1、ALP mRNA表達(dá)下降(P0.05),10-5、10-6、10-7mol/Lβ-Ecd能不同程度干預(yù)細(xì)胞降低的基因mRNA表達(dá)水平。GC模型組細(xì)胞β-catenin蛋白表達(dá)升高(P0.05),10-5mol/Lβ-Ecd能抑制GC對(duì)細(xì)胞的影響。結(jié)論:超生理劑量GC抑制體外培養(yǎng)成骨細(xì)胞的分化與活性,可能與介導(dǎo)Wnt/β-catenin信號(hào)途徑有關(guān);一定劑量β蛻皮甾酮?jiǎng)t能不同程度地干預(yù)糖皮質(zhì)激素對(duì)成骨細(xì)胞的影響。
[Abstract]:Aim: to investigate the effects of different doses of glucocorticoid (GC) on osteoblast activity and differentiation in vitro and the intervention of 尾-ecdysterone (尾-Ecd), an active component of Achyranthes bidentata, in order to explore the mechanism of osteoporosis induced by GC. Methods: osteoblasts were differentiated from bone marrow stromal cells and treated with dexamethasone (Dex) at different dosages and time. After MTT assay, the cells were divided into 7 groups: normal control group, 1 脳 10-5mol/L GC model group. GC 10-5mol/L RU486 group, GC 10-5mol/L alendronate (ALN) group, GC 10-5mol/L 尾 -Ecd group, GC 10-6mol/L 尾 -Ecd group, GC 10-7mol/L 尾 -Ecd group and. Real time PCR group were used to detect the mRNA expression. The expression of 尾-catenin protein was detected by Western Blot. Results: low dose of Dex (鈮，

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