【摘要】：目的:通過雙黃消痤丸對(duì)兔耳痤瘡模型毛囊面積、皮脂腺數(shù)量和皮脂腺直徑影響的研究,探討雙黃消痤丸對(duì)機(jī)體抗角化和改善皮脂腺的作用機(jī)理,為臨床治療痤瘡提供實(shí)驗(yàn)依據(jù)。方法:將48只12周齡的新西蘭雄性大白兔隨機(jī)分為6組:雙黃消痤丸高、中、低劑量組、陽(yáng)性對(duì)照組、模型對(duì)照組、空白對(duì)照組,每組各8只。除空白對(duì)照組以外,其余各組均建立兔耳痤瘡模型。造模成功后,高、中、低劑量組各給藥量分別為1.68g/kg、0.84g/kg、0.42g/kg,陽(yáng)性對(duì)照組(丹參酮膠囊組)給藥量為0.187g/kg,空白組與模型組給予同體積的0.9%氯化鈉溶液,日一次,連續(xù)14天。于末次給藥20h后,在兔的耳緣靜脈中注射20ml的空氣,將其處死。然后迅速用1cm打孔器在右耳標(biāo)記處取皮膚組織塊,并用10%福爾馬林進(jìn)行固定,石蠟包埋,切片,HE染色,于光鏡下觀察其組織形態(tài)學(xué)的改變,記錄毛囊面積、皮脂腺數(shù)量和皮脂腺直徑。結(jié)果:1、雙黃消痤丸各組與丹參酮膠囊對(duì)照組均能縮小兔耳痤瘡模型皮脂腺的直徑,且雙黃消痤丸高劑量組療效優(yōu)于低劑組(P0.01);2、雙黃消痤丸高劑組能使痤瘡模型中正常的皮脂腺數(shù)量明顯增多,使正常結(jié)構(gòu)的皮脂腺數(shù)量趨于正常水平(P0.05);3、雙黃消痤丸高劑量組對(duì)痤瘡模型毛囊面積影響顯著,能顯著縮小增生的毛囊面積(P0.01)。結(jié)論:雙黃消痤丸能減少實(shí)驗(yàn)兔耳皮脂腺數(shù)量、縮小皮脂腺的直徑及毛囊面積,對(duì)調(diào)節(jié)皮脂腺分泌量、縮小毛囊、抗角化等方面的效果顯著。
[Abstract]:Objective: to study the effects of Shuanghuangxiaocuo pills on hair follicle area, sebaceous gland quantity and sebaceous gland diameter in rabbit ear acne model, and to explore the mechanism of Shuanghuangxiaocuo pill on anti-keratosis and improvement of sebaceous gland. To provide experimental basis for clinical treatment of acne. Methods: 48 New Zealand male rabbits aged 12 weeks were randomly divided into 6 groups: Shuanghuangxiaocuo pills high, middle and low dose groups, positive control group, model control group and blank control group, each group had 8 rabbits. Except the blank control group, all the other groups established the rabbit ear acne model. After the establishment of the model, the dosage of high, medium and low dose groups were 1.68 g / kg / kg 0.84 g / kg and 0.187 g / kg, respectively. The control group (tanshinone capsule group) was given 0.187 g / kg of sodium chloride solution, and the blank group and model group were given 0.9% sodium chloride solution in the same volume once a day for 14 days. After the last administration for 20 hours, 20ml was injected into the auricular vein of rabbits and killed. Then the skin tissue mass was quickly taken from the right ear label with 1cm perforator, fixed with 10% formalin, embedded in paraffin, stained with HE. The histomorphology of the tissue was observed under light microscope, and the hair follicle area was recorded. The number of sebaceous glands and the diameter of sebaceous glands. Results: the diameter of sebaceous gland of acne model was reduced in each group of Shuanghuangxiaocuo pill group and tanshinone capsule control group. The therapeutic effect of Shuanghuangxiaocuo pills in high dose group was better than that in low dose group (P0.01), and Shuanghuangxiaocuo pills high dose group could significantly increase the number of normal sebaceous glands in acne model. The number of sebaceous glands of normal structure tended to normal level (P0.05). The high dose group of Shuanghuangxiaocuo pills had a significant effect on the hair follicle area of acne model and could significantly reduce the accretive hair follicle area (P0.01). Conclusion: Shuanghuangxiaocuo pills can reduce the number of sebaceous glands, reduce the diameter of sebaceous glands and the area of hair follicles, regulate the secretion of sebaceous glands, reduce hair follicles and resist keratosis.
【學(xué)位授予單位】：黑龍江省中醫(yī)藥科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5;R-332

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