本文選題：甲型流感病毒 + 新型HN��； 參考：《現(xiàn)代預(yù)防醫(yī)學(xué)》2013年06期

【摘要】：目的探討新型H1N1流感病毒從2009年在蘇州出現(xiàn)至2011年基本消失的整個過程中,蘇州分離株的神經(jīng)氨酸酶(Neuraminidase,NA)的分子特征及其基因變異和發(fā)展規(guī)律。方法提取蘇州分離株的RNA,以WHO推薦的引物擴(kuò)增NA基因并測序。以WHO推薦的季節(jié)性H1N1和新型H1N1疫苗株為參照,采用生物信息學(xué)軟件對蘇州毒株的NA進(jìn)行詳細(xì)的分子特征分析,并與全球及全國的代表性毒株進(jìn)行序列進(jìn)化分析。結(jié)果與疫苗株相比,5株蘇州株的核苷酸序列同源性均值為99.64%,氨基酸序列同源性均值為99.36%。5株蘇州株中都沒有發(fā)現(xiàn)抗原位點(diǎn)的改變,但4株在耐藥性相關(guān)位點(diǎn)附近出現(xiàn)N248D突變,還有1株2011年株增加了1個新的潛在糖基化位點(diǎn)。結(jié)論首次詳細(xì)分析了蘇州地區(qū)2009年以來H1N1型流感病毒NA的分子特征,表明蘇州毒株出現(xiàn)了一個新的潛在糖基化位點(diǎn),但抗原性和藥物敏感性均保持穩(wěn)定。
[Abstract]:Objective to investigate the molecular characteristics, gene variation and development of neuraminidase (na) of the new H1N1 influenza virus in Suzhou from 2009 to 2011. Methods RNA was extracted from Suzhou isolate. Na gene was amplified and sequenced with primers recommended by WHO. Taking the seasonal H1N1 and new H1N1 vaccine strains recommended by WHO as reference, the molecular characteristics of na of Suzhou strain were analyzed by bioinformatics software, and the sequence evolution analysis was carried out with the representative strains in the world and the whole country. Results the average nucleotide sequence homology and amino acid sequence homology were 99.64 and 99.36.5, respectively, compared with the vaccine strains. However, N248D mutations were found in 4 strains near drug-resistance related sites. Another 2011 strain added a new potential glycosylation site. Conclusion the molecular characteristics of H1N1 influenza virus na in Suzhou region since 2009 were analyzed in detail for the first time. The results showed that there was a new potential glycosylation site in Suzhou strain, but the antigenicity and drug sensitivity remained stable.
【作者單位】： 蘇州市疾病預(yù)防控制中心;上海市疾病預(yù)防控制中心;
【基金】：江蘇省預(yù)防醫(yī)學(xué)課題(Y201023) 蘇州市科教興衛(wèi)課題(SWKQ1032)
【分類號】：R373

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