本文選題：血吸蟲 + 日本��； 參考：《中國病原生物學雜志》2013年12期

【摘要】：目的改進檢測預(yù)警哨鼠血清抗日本血吸蟲23ku膜蛋白大親水肽段(Sj23HD)IgG抗體的Western blot方法,并觀察其現(xiàn)場應(yīng)用價值。方法預(yù)先制備轉(zhuǎn)印有重組Sj23HD蛋白的NC膜條,采用化學發(fā)光Western blot檢測哨鼠血清抗Sj23HD IgG,觀察方法的穩(wěn)定性和敏感性,并對2012年江蘇省潛在血吸蟲感染高風險水域監(jiān)測預(yù)警哨鼠回收后第14、21、35d血樣進行檢測,同時以ELISA檢測方法作為對照,對免疫檢測結(jié)果與解剖查蟲結(jié)果進行統(tǒng)計學分析。結(jié)果改進后的Western blot操作簡便,比常規(guī)方法節(jié)省1~2周時間。預(yù)制NC膜條至少可低溫保存6個月,其敏感性與新鮮制備的NC膜條的敏感性相似。使用改進后的Western blot方法檢測預(yù)警哨鼠14、21和35d血清標本,陽性率分別為0.90%,3.50%和5.06%,ELISA陽性率分別為0.45%、3.00%和5.06%,差異無統(tǒng)計學意義(P0.05)。兩種免疫學方法的敏感性均高于解剖查蟲法。結(jié)論改進后的Western blot操作簡便,敏感性高,可用于預(yù)警哨鼠血吸蟲感染檢測。
[Abstract]:Objective to improve the Western blot method for detection of anti-Schistosoma japonicum 23ku membrane protein large hydrophilic peptide fragment (Sj23HD) IgG antibody in early warning sentinel rat serum, and to observe its application value in the field. Methods the NC membrane strips with recombinant Sj23HD protein were prepared in advance. The serum anti-Sj23HD IgG of sentinel mice was detected by chemiluminescence Western blot. The stability and sensitivity of the method were observed. The blood samples of sentinel rats collected from potential Schistosoma japonicum infection high risk areas in Jiangsu Province in 2012 were detected on day 1421, and Elisa was used as control. The results of immunoassay and dissection were analyzed statistically. Results the improved Western blot was easy to operate and saved 1 ~ 2 weeks compared with the conventional method. The prefabricated NC strips can be preserved at least 6 months at low temperature, and the sensitivity of prefabricated NC strips is similar to that of freshly prepared NC strips. The positive rates of Elisa were 0.90% and 5.06%, respectively, and the positive rates were 0.45% and 5.06%, respectively. There was no significant difference between the two groups (P0.05). The sensitivity of the two immunological methods was higher than that of the dissecting method. Conclusion the improved Western blot is simple and sensitive, and can be used to detect schistosomiasis infection in sentinel mice.
【作者單位】： 衛(wèi)生部重要寄生蟲病預(yù)防與控制技術(shù)重點實驗室 江蘇省寄生蟲分子生物學重點實驗室 江蘇省血吸蟲病防治研究所;衛(wèi)生部重要寄生蟲病預(yù)防與控制技術(shù)重點實驗 室江蘇省寄生蟲分子生物學重點實驗室 江蘇省血吸蟲病防治研究所;衛(wèi)生部重要寄生蟲病預(yù)防與控制技術(shù)重點實驗室江蘇省寄生蟲分子生物學重點實驗室 江蘇省血吸蟲病防治研究所;
【基金】：國家傳染病重大專項(No.2012ZX10004-220) 國家自然科學基金項目(No.30972581,81201316) 江蘇省自然科學基金項目(No.BK2008110,BK2012544) 江蘇省衛(wèi)生廳科技項目(No.H200738,H201066) 江蘇省醫(yī)學重點人才項目(No.RC2011094) 江蘇省興衛(wèi)工程基金項目(No.ZX201108) 江蘇省血吸蟲病、地方病及寄生蟲病項目(No.X201115)
【分類號】：R392.1

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