本文選題：脂多糖 + 星形膠質(zhì)細胞��； 參考：《中國免疫學雜志》2013年10期

【摘要】：目的:探討內(nèi)毒素脂多糖(Lipopolysaccharide,LPS)對大鼠星形膠質(zhì)細胞熱休克蛋白A12B(Heat shock proteins A 12B,HSPA12B)表達變化和細胞定位的影響。方法:用不同濃度(0.01、0.1、1、10μg/ml)和同一濃度(1μg/ml)不同時間(1、2、4、6、8、12、24小時)的LPS刺激星形膠質(zhì)細胞,分別用RT-PCR和Western blot法檢測星形膠質(zhì)細胞HSPA12B的mRNA和蛋白質(zhì)水平表達情況,間接免疫熒光雙標法檢測HSPA12B在星形膠質(zhì)細胞中的細胞定位。結果:用LPS 1μg/ml刺激1小時后,HSPA12B mRNA的表達增加,4小時表達活性最高。HSPA12B蛋白水平表達變化與mRNA水平變化相一致。免疫細胞化學證明LPS誘導星形膠質(zhì)細胞HSPA12B的表達定位在胞核。結論:LPS可誘導星形膠質(zhì)細胞HSPA12B表達上調(diào)。HSPA12B可作為一種炎癥反應蛋白參與炎癥反應過程。
[Abstract]:Aim: to investigate the effect of lipopolysaccharide (LPS) on the expression of heat shock protein (A12B(Heat shock proteins A 12B) and its cellular localization in rat astrocytes. Methods: the astrocytes were stimulated with LPS at different concentrations of 0. 01 ~ 0. 1 ~ 1 ~ (1 渭 g / ml) and 1 渭 g / ml of the same concentration of 1 渭 g / ml). The expression of mRNA and protein in astrocytes was detected by RT-PCR and Western blot methods. The localization of HSPA12B in astrocytes was detected by indirect immunofluorescence double labeling method. Results: after 1 hour stimulation with LPS 1 渭 g/ml, the expression of HSPA12B mRNA was increased. The expression of HSPA12B protein was the highest at 4 hours. The change of HSPA12B protein level was consistent with the change of mRNA level. Immunocytochemistry showed that the expression of HSPA12B in astrocytes induced by LPS was localized in the nucleus. Conclusion the up-regulation of HSPA12B expression and HSPA12B may be involved in the process of inflammation as a kind of inflammatory response protein.
【作者單位】： 浙江省紹興市人民醫(yī)院;浙江大學紹興醫(yī)院輸血科;南通大學基礎醫(yī)學院免疫與微生物實驗室;
【分類號】：R363

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