本文關(guān)鍵詞： 帕金森病 疾病模型 動(dòng)物 羥多巴胺 電刺激 蒼白球 谷氨酸 γ氨基丁酸 大鼠 Sprague-Dawley 腳橋核　出處：《天津醫(yī)藥》2013年07期 　論文類(lèi)型：期刊論文

【摘要】：目的通過(guò)觀察高頻電刺激帕金森(PD)模型大鼠腳橋核(PPN)對(duì)蒼白球內(nèi)側(cè)部(GPi)氨基酸類(lèi)遞質(zhì)及神經(jīng)元放電的影響,探討電刺激PPN治療PD的機(jī)制。方法將70只SD雄性大鼠隨機(jī)分為對(duì)照組30只,PD模型組40只。大腦右側(cè)黑質(zhì)致密部?jī)牲c(diǎn)注射6-羥基多巴胺建立PD模型,應(yīng)用細(xì)胞外單位記錄觀察高頻電刺激PPN對(duì)GPi神經(jīng)元放電的影響。腦內(nèi)微透析收集損毀側(cè)GPi腦脊液,結(jié)合高效液相色譜檢測(cè)氨基酸類(lèi)遞質(zhì)的變化。結(jié)果高頻電刺激PPN,對(duì)照組及PD模型組大鼠GPi神經(jīng)元的反應(yīng)均以興奮性為主,且平均放電頻率均較刺激前明顯增高(P㩳0.01);高頻電刺激PPN,GPi內(nèi)谷氨酸水平較刺激前明顯減少(P㩳0.01),γ-氨基丁酸水平較刺激前無(wú)明顯變化(P㧐0.05)。結(jié)論高頻電刺激PPN可增強(qiáng)GPi神經(jīng)元的電活動(dòng),降低GPi內(nèi)谷氨酸的水平,其興奮GPi神經(jīng)元的效應(yīng)可能是通過(guò)PPN-GPi直接通路或其他間接通路實(shí)現(xiàn)的。
[Abstract]:Objective to observe the effects of high frequency electrical stimulation on the amino acid transmitters and neuronal discharges of GPiG in the medial part of globus pallidus. Methods 70 male SD rats were randomly divided into control group (n = 30) and control group (n = 30). PD model group (n = 40). PD model was established by two points injection of 6-hydroxydopamine into the right substantia nigra compact part of the brain. The effects of high frequency electrical stimulation (PPN) on the discharge of GPi neurons were observed by extracellular unit recording. Cerebrospinal fluid (CSF) was collected from the damaged side of GPi by intracerebral microdialysis. Results the responses of GPi neurons to high frequency electrical stimulation, control group and PD model group were mainly excitatory. The average discharge frequency was significantly higher than that before stimulation. Glutamic acid levels in PPNG GPI were significantly reduced by high frequency electrical stimulation compared with those before stimulation. The levels of 緯 -aminobutyric acid were not significantly changed compared with those before stimulation. Conclusion High frequency electrical stimulation of PPN can enhance the electrical activity of GPi neurons and decrease the level of glutamate in GPi. The effect of stimulating GPi neurons may be realized through PPN-GPi direct pathway or other indirect pathway.
【作者單位】： 遼寧醫(yī)學(xué)院生理學(xué)教研室;
【基金】：遼寧省教育廳科研項(xiàng)目(項(xiàng)目編號(hào):L2010252)
【分類(lèi)號(hào)】：R338
【正文快照】： 帕金森病(PD)是以中腦黑質(zhì)多巴胺能神經(jīng)元變性缺失為主要病理變化的漸進(jìn)性神經(jīng)系統(tǒng)退行性疾病。高頻電刺激(high frequency stimulation,HFS)丘腦底核(subthalamic nucleus,STN)、蒼白球內(nèi)側(cè)部(GPi)等基底神經(jīng)節(jié)核團(tuán)是功能外科治療PD的首選方法[1],但是該方法對(duì)步態(tài)障礙、靜

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