天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

當(dāng)前位置:主頁 > 醫(yī)學(xué)論文 > 呼吸病論文 >

Th17細(xì)胞及其細(xì)胞因子白細(xì)胞介素-17在粉塵螨致敏哮喘小鼠中的變化

發(fā)布時(shí)間:2019-07-20 07:43
【摘要】:目的探討Th17細(xì)胞及其細(xì)胞因子白細(xì)胞介素-17(IL-17)在粉塵螨致敏哮喘小鼠中的變化及其意義。方法 20只BALB/c小鼠隨機(jī)均分為哮喘組和健康對(duì)照組。哮喘組小鼠于第0、7和14天每鼠腹腔注射200μl致敏液[含粉塵螨粗浸液提取物50μg,Al(OH)32 mg]致敏。末次免疫后1周采用粉塵螨粗浸液提取物連續(xù)進(jìn)行滴鼻激發(fā),每天1次,每次50μg,激發(fā)7次,健康對(duì)照組給予等體積的PBS[含Al(OH)32 mg]處理。末次激發(fā)后24 h內(nèi)處死小鼠,取血清和肺泡灌洗液,無菌取脾臟。ELISA檢測(cè)血清中Ig G1、Ig E和肺泡灌洗液中IL-17的含量;流式細(xì)胞儀檢測(cè)脾臟中Th17細(xì)胞百分率。結(jié)果哮喘組小鼠血清中Ig G1和Ig E水平分別為(0.10±0.01)pg/ml和(1.15±0.10)pg/ml,高于健康對(duì)照組的(0.06±0.01)pg/ml和(0.04±0.01)pg/ml(P0.05);哮喘組小鼠肺泡灌洗液IL-17水平(85.13±2.36)pg/ml高于健康對(duì)照組(48.27±4.14)pg/ml(P0.01);哮喘組小鼠脾臟Th17細(xì)胞百分率(5.19±0.68)%高于健康對(duì)照組(0.95±0.19)%(P0.01),且脾臟Th17細(xì)胞百分率與肺泡灌洗液中IL-17水平呈正相關(guān)(r=0.851,P0.01)。結(jié)論粉塵螨致敏哮喘小鼠較健康小鼠肺泡灌洗液IL-17水平和脾臟Th17細(xì)胞數(shù)量均升高。
[Abstract]:Objective to investigate the changes and significance of Th17 cells and their cytokine IL-17 (IL-17) in asthma mice sensitized by Dermatophagoides farinae. Methods 20 BALB/c mice were randomly divided into asthma group and healthy control group. In the asthma group, 200 渭 l sensitized solution (50 渭 g, Al (OH) 32 mg) was injected intraperitoneally on the 0th, 7th and 14th day. One week after the last immunization, the crude extract of Dermatophagoides farinae was used for nasal stimulation once a day, 50 渭 g each time for 7 times. The healthy control group was treated with the same volume of PBS [containing Al (OH) 32 mg]. The mice were killed within 24 hours after the last challenge, serum and alveolar lavage fluid were taken, and spleen was taken aseptically. The contents of Ig G1, IgE and IL-17 in serum and alveolar lavage fluid were detected by Elisa, and the percentage of Th17 cells in spleen was detected by flow cytometry. Results the serum levels of Ig G 1 and Ig E in asthma group were (0.10 鹵0.001) pg/ml and (1.15 鹵0.10) pg/ml, respectively, which were higher than those in healthy control group (0.006 鹵0.01pg/ml and 0.004 鹵0.01pg/ml, P 0.05), and the levels of IL-17 in alveolar lavage fluid in asthma group (85.13 鹵2.36) pg/ml were higher than those in healthy control group (48.27 鹵4.14) pg/ml (P 0.01), and the levels of IL-17 in alveolar lavage fluid in asthma group were significantly higher than those in healthy control group (P < 0.01). The percentage of Th17 cells in spleen of asthma group (5.19 鹵0.68)% was higher than that of healthy control group (0.95 鹵0.19)% (P 0.01), and the percentage of Th17 cells in spleen was positively correlated with the level of IL-17 in alveolar lavage fluid (r 鈮,

本文編號(hào):2516576

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/huxijib/2516576.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶80105***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
日本高清一道一二三区四五区| 亚洲妇女黄色三级视频| 深夜视频在线观看免费你懂| 老富婆找帅哥按摩抠逼视频| 中文字幕日韩欧美亚洲午夜| 日系韩系还是欧美久久| 久久这里只精品免费福利| 国产高清精品福利私拍| 欧美精品一区久久精品| 黄色在线免费高清观看| 亚洲视频一区二区久久久| 国产一区二区三区成人精品| 亚洲熟女熟妇乱色一区| 久久精品国产熟女精品| 国产一区二区不卡在线视频| 果冻传媒精选麻豆白晶晶| 91人妻丝袜一区二区三区| 日韩欧美三级中文字幕| 日韩精品免费一区三区| 人妻少妇系列中文字幕| 国产精品欧美日韩中文字幕| 色婷婷激情五月天丁香| 中文字幕日韩欧美亚洲午夜| 亚洲av首页免费在线观看| 亚洲天堂精品一区二区| 日韩精品区欧美在线一区| 婷婷亚洲综合五月天麻豆| 精品一区二区三区免费看| 亚洲国产成人久久一区二区三区| 在线观看日韩欧美综合黄片| 中文字幕在线区中文色 | 丰满人妻少妇精品一区二区三区| 精品久久少妇激情视频| 国产一二三区不卡视频| 欧美精品久久男人的天堂| 久久热九九这里只有精品| 日韩无套内射免费精品| 久久成人国产欧美精品一区二区| 男女午夜福利院在线观看| 免费观看在线午夜视频| 欧美国产在线观看精品|