發(fā)布時間：2019-06-12 05:30

【摘要】：目的探討ARDS大鼠吸入高濃度氧氣后肺組織氧化應激的變化。方法實驗動物分為正常大鼠組和ARDS大鼠組,每組30只,每組再以吸入空氣、60%氧氣、100%氧氣各分成三個亞組,每個亞組10只。72 h后留取動脈血行血氧分壓(PO2)及丙二醛(MDA)、超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、單胺氧化酶(MAO)測定;肺組織行HE染色觀察Smith評分,RT-PCR檢測Ⅱ型肺泡上皮細胞(AECⅡ)特異性肺泡表面活性蛋白C(SPC)和Ⅰ型肺泡上皮細胞(AECⅠ)特異性水通道蛋白5(AQP5)的mRNA表達。結(jié)果各組大鼠吸氧72 h后均存活。與正常大鼠空氣亞組比較,正常大鼠100%氧氣亞組Smith評分升高(3.77±1.20比0.83±0.26),P0.05;MDA升高(2.39±0.92比0.82±0.31),P0.05;SOD下降(51.99±21.73比86.44±26.38),P0.05;AQP5 mRNA下降(0.67±0.25比0.91±0.28),P0.05;ARDS大鼠空氣亞組的Smith評分升高(11.65±3.82比0.83±0.26),P0.05;MDA升高(5.73±2.43比0.82±0.31),P0.05;SOD下降(45.71±14.68比86.44±26.38),P0.05;CAT下降(0.52±0.23比0.85±0.34),P0.05;AQP5 mRNA下降(0.28±0.12比0.91±0.28),P0.05;SPC mRNA下降(0.33±0.14比0.72±0.28),P0.05。ARDS組大鼠空氣亞組的Smith評分(11.65±3.82比3.77±1.20),P0.05和AQP5 mRNA(0.28±0.12比0.67±0.25),P0.05;與正常大鼠100%氧氣亞組差異顯著。MAO在各組內(nèi)和組間均差異無顯著性(P0.05)。ARDS組大鼠動脈血氧分壓100%氧氣亞組高于60%氧氣亞組(112.86±26.32比86.94±21.07),P0.05,60%氧氣亞組高于空氣亞組(86.94±21.07比65.05±15.19),P0.05。在ARDS組內(nèi)與空氣亞組比較,60%氧氣亞組(0.43±0.18 VS 0.28±0.12),P0.05和100%氧氣亞組(0.47±0.23比0.28±0.12),P0.05的AQP5 mRNA均有顯著性升高;100%氧氣亞組的Smith評分降低(7.88±2.53比11.65±3.82),P0.05,SPC mRNA升高(0.58±0.25比0.33±0.14),P0.05。結(jié)論高濃度氧氣吸入和ARDS均可以影響氧自由基,氧自由基并不是高濃度氧的特征性產(chǎn)物,難以作為客觀有效的氧毒性監(jiān)測指標;高濃度氧氣吸入對成年大鼠的肺部損害輕微且沒有特異性;ARDS大鼠吸入100%氧氣后沒有出現(xiàn)疊加損害,反而比60%氧氣更好地改善了肺部Smith評分和動脈血氧分壓,增加了SPCmRNA和AQP5mRNA的表達,提示高濃度氧氣對ARDS可能存在更為復雜的修復機制。
[Abstract]:Objective to investigate the changes of oxidative stress in lung tissue of ARDS rats after inhaling high concentration of oxygen. Methods the experimental animals were divided into normal rat group and ARDS rat group with 30 rats in each group. Each group was divided into three subgroups with inhaled air, 60% oxygen and 100% oxygen, 10 rats in each subgroup. 72 hours later, arterial blood was taken for partial pressure of oxygen (PO2) and malondialdehyde (MDA),) (SOD), catalase (CAT), monoamine oxidase (MAO). The Smith score was observed by HE staining. The mRNA expression of specific alveolar surface active protein C (SPC) and type I alveolar epithelial cell (AEC 鈪，

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