【摘要】：目的：探討腫瘤壞死因子-α(TNF-a)、血管內(nèi)皮生長因子(VEGF)、基質(zhì)金屬蛋白酶-9(MMP-9)在大鼠自身免疫性肺氣腫中的作用,為研究其發(fā)病機(jī)制及尋求新的治療方法提供理論依據(jù)。 方法：清潔級SD大鼠20只隨機(jī)分為2組：佐劑對照組(n=10)和自身免疫性肺氣腫模型組(簡稱模型組,n=10)。將原代培養(yǎng)的人臍靜脈內(nèi)皮細(xì)胞及佐劑注入大鼠腹腔內(nèi)建立自身免疫性肺氣腫模型,佐劑對照組只給予佐劑大鼠腹腔內(nèi)注射。兩組大鼠均取肺組織切片行蘇木素-伊紅染色(HE)觀察病理學(xué)改變,并定量測定肺平均內(nèi)襯間隔(MLI)、平均肺泡數(shù)(MAN),以ELISA檢測支氣管肺泡灌洗液(BALF)中的TNF-a、VEGF和MMP-9含量。 結(jié)果：(1)模型組MLI比佐劑對照組增高,而其MAN比佐劑對照組降低,差異均有統(tǒng)計(jì)學(xué)意義(P均0.05)。 (2)模型組BALF中MMP-9含量較佐劑對照組升高(P0.05),而兩組TNF-α含量差異無統(tǒng)計(jì)學(xué)意義(P0.05)。 (3)模型組BALF中VEGF含量較佐劑對照組降低(P0.05),差異有統(tǒng)計(jì)學(xué)意義。 結(jié)論：(1)將臍靜脈內(nèi)皮細(xì)胞注入正常大鼠腹腔即可以建立自身免疫性肺氣腫模型。 (2)MMP-9含量升高、VEGF含量減低可能參與大鼠自身免疫性肺氣腫的形成,而TNF-a與該病的形成無關(guān)。
[Abstract]:Objective: to investigate the role of tumor necrosis factor-偽 (TNF-a) and vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) in autoimmune emphysema in rats. It provides a theoretical basis for the study of its pathogenesis and the search for new therapeutic methods. Methods: twenty clean grade SD rats were randomly divided into two groups: adjuvant control group (n = 10) and autoimmune emphysema model group (n = 10). The model of autoimmune emphysema was established by injecting primary cultured human umbilical vein endothelial cells and adjuvant into the abdominal cavity of rats. The adjuvant control group was given only intraperitoneal injection of adjuvant rats. The pathological changes of lung tissue were observed by hematoxylin-eosin staining (HE), and the mean alveolar number (MAN),) of mean lining interval (MLI),) was measured quantitatively. The TNF-a, in bronchoalveolar lavage fluid (BALF) was detected by ELISA. VEGF and MMP-9 contents. Results: (1) the MLI of the model group was higher than that of the adjuvant control group, while the MAN of the model group was lower than that of the adjuvant control group. (2) the content of MMP-9 in BALF of the model group was higher than that of the adjuvant control group (P0.05), but there was no significant difference between the two groups in the content of TNF- 偽 (P0.05). (3) the content of VEGF in BALF of model group was lower than that of adjuvant control group (P0.05). Conclusion: (1) the model of autoimmune emphysema can be established by injecting umbilical vein endothelial cells into the abdominal cavity of normal rats. (2) the increase of MMP-9 content and the decrease of VEGF content may be involved in the formation of autoimmune emphysema in rats, but TNF-a is not related to the formation of autoimmune emphysema.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R563

【引證文獻(xiàn)】

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1 張朝杰;張程;路蘋;歐陽章宏;張湘燕;;甲潑尼龍琥珀酸鈉對自身免疫性肺氣腫大鼠TNF-a、MMP-9、IL-8及VEGF水平的影響[J];貴州醫(yī)藥;2014年04期

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